Vancomycin-resistant enterococci (VRE) in meat and environmental samples

Messi, Patrizia; Guerrieri, Elisa; Niederhäusern, Simona De; Sabia, Carla; Bondi, Moreno

doi:10.1016/j.ijfoodmicro.2005.08.026

Cited by 61 publications

(41 citation statements)

References 26 publications

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“…and 6.0% (6 of 100 samples) in meatballs and meat cuts. VRE were isolated from several foodstuffs in many other countries such as Italy (10,19), Spain (9,20), Germany (13), Korea (21), Denmark (16), and Brazil …”

Section: Discussionmentioning

confidence: 99%

Prevalence and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods

Çetinkaya¹,

Muş²,

Soyutemiz³

et al. 2013

Turk J Vet Anim Sci

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The aim of this work is to identify the parameters influencing the flame behavior. Three different species (Pinus pinaster, Pinus halepensis and Erica arborea) involved in forest fire were crushed to decrease the geometric effects on their combustion. The flaming behavior is studied experimentally from unsteady, axisymmetric, non-premixed laminar flames. The distribution of temperature, the flame geometry, the mass loss as well as the gases released by the fuels are measured. This study confirms the role of the mass burning rate of fuels on the flame dynamics. However, this work highlights a second influence for laminar flames: the composition of degradation gases. It affects the flame geometry and changes the combustion kinetics in the reaction zone.

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Section: Discussionmentioning

confidence: 99%

Prevalence and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods

Çetinkaya¹,

Muş²,

Soyutemiz³

et al. 2013

Turk J Vet Anim Sci

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“…Enterococci are not important food-borne pathogens, but livestock and poultry can serve as reservoirs for drug-resistant strains of Enterococcus, which may then enter the human food chain and cause serious infections (4,14,15,37,44). There is increasing concern about the emergence of multiple-antibiotic-resistant enterococci and the presence of vancomycin-resistant enterococci in nonhuman reservoirs (1,14,18,20,52).…”

mentioning

confidence: 99%

Characterization of Monolaurin Resistance in Enterococcus faecalis

Dufour¹,

Manson²,

Bremer

et al. 2007

Appl Environ Microbiol

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There is increasing concern regarding the presence of vancomycin-resistant enterococci in domestically farmed animals, which may act as reservoirs and vehicles of transmission for drug-resistant enterococci to humans, resulting in serious infections. In order to assess the potential for the use of monolaurin as a food preservative, it is important to understand both its target and potential mechanisms of resistance. A Tn917 mutant library of Enterococcus faecalis AR01/DGVS was screened for resistance (MIC, >100 g/ml) to monolaurin. Three mutants were identified as resistant to monolaurin and were designated DGRM2, DGRM5, and DGRM12. The gene interrupted in all three mutants was identified as traB, which encodes an E. faecalis pheromone shutdown protein and whose complementation in trans restored monolaurin sensitivity in all three mutants. DGRM2 was selected for further characterization. E. faecalis DGRM2 showed increased resistance to gentamicin and chloramphenicol (inhibitors of protein synthesis), while no difference in the MIC was observed with the cell wall-active antibiotics penicillin and vancomycin. E. faecalis AR01/DGVS and DGRM2 were shown to have similar rates (30% cell lysis after 4 h) of cell autolytic activity when activated by monolaurin. Differences in cell surface hydrophobicity were observed between the wild type and the mutant, with the cell surface of the parent strain being significantly more hydrophobic. Analysis of the cell wall structure of DGRM2 by transmission electron microscopy revealed an increase in the apparent cell wall thickness and contraction of its cytoplasm. Taken together, these results suggest that the increased resistance of DGRM2 was due to a change in cell surface hydrophobicity, consequently limiting the diffusion of monolaurin to a potential target in the cytoplasmic membrane and/or cytoplasm of E. faecalis.

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“…Conversely, a study performed by Varela et al (2013) demonstrated the detection of vancomycin-resistant enterococci from hospital and urban wastewater samples. Again, the VRE prevalence was recorded as 12.9% in the aquatic environmental samples in Korea by Nam et al (2013) and as 25.6% in superficial water samples by Messi et al (2006). Resistance to teicoplanin was not found in any of the Enterococcus spp.…”

Section: Resultsmentioning

confidence: 89%

“…Recently, gelE, efaA, ace and asa1 genes were reported to occur in Enterococcus isolates from surface waters (Sidhu et al, 2014). A study made by Messi et al (2006) suggested that 3 (0.7%) isolates from superficial waters belonged to the vanA, 53 (13.7%) to the vanB and 43 (11.1%) to the vanC phenotype.…”

Section: Resultsmentioning

confidence: 99%

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Investigation of the Presence and Virulence Traits of Vancomycin-Resistant Enterococcus in Water Samples

Muş¹

2017

J Food Health Sci

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Abstract:The aim of this study was to determine the incidence of vancomycin-resistant enterococci (VRE) in tap and artesian well waters and to detect the vancomycin resistance genes and virulence genes of the isolates obtained from the samples. For this purpose, 200 samples (119 tap and 81 artesian well waters) were collected from several water supplies during November 2013 and June 2015 period in Bursa province. Seven isolates were recovered from artesian well waters and confirmed as Enterococcus by PCR method. E-test performed for vancomycin and teicoplanin MIC values indicated that only two isolates had the intermediate-level (8 μg/mL) resistance to vancomycin. No resistance was observed to teicoplanin in any of these isolates by Etest. All of 7 isolates were tested for vancomycin resistance genes (vanA, vanB and vanC) and virulence genes (gelE, agg, esp and ace). The results showed that enterococci isolates had no these genes. The present study suggested that the presence of the intermediate level VRE in artesian well waters and, also the waters from environmental supplies near human and animal niches could be play a role as potential reservoirs for enterococci having several types of resistance to vancomycin. Also, vancomycin resistant strains can be possible the spread in environment and also the transmission to human and animals through contaminated water sources.

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Vancomycin-resistant enterococci (VRE) in meat and environmental samples

Cited by 61 publications

References 26 publications

Prevalence and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods

Prevalence and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods

Characterization of Monolaurin Resistance in Enterococcus faecalis

Investigation of the Presence and Virulence Traits of Vancomycin-Resistant Enterococcus in Water Samples

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