Vancomycin dependence in a vanA-producing Enterococcus avium strain with a nonsense mutation in the natural D-Ala-D-Ala ligase gene

Sifaoui, Farid; Gutmann, Laurent

doi:10.1128/aac.41.6.1409

Cited by 32 publications

(17 citation statements)

References 6 publications

(7 reference statements)

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“…Such dependence does not mean that bacteria use antibiotics as a food resource (Dantas et al, 2008). The causes of antibiotic-dependent growth have been studied in vancomycin-resistant enterococci (Baptista et al, 1997;Sifaoui & Gutmann, 1997;Van Bambeke et al, 1999). The causes of antibiotic-dependent growth have been studied in vancomycin-resistant enterococci (Baptista et al, 1997;Sifaoui & Gutmann, 1997;Van Bambeke et al, 1999).…”

Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

“…Because dependence is usually linked to resistance, the underlying reason might be the metabolic rewiring produced by the resistance mechanism now only allowing the growth of the bacterium in the presence of the antibiotic. Thus, the ddl gene encoding the host D-Ala : D-Ala ligase accumulates mutations and bacteria can only grow in the presence of the antibiotic because it induces expression of the alternative, antibiotic-resistance-linked ligase (Baptista et al, 1997;Sifaoui & Gutmann, 1997;Van Bambeke et al, 1999). As stated above, resistance to glycopeptides involves the formation of a D-Ala-D-Lac depsipeptide.…”

Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

“…Under strong selective pressure and in the presence of the vancomycin-resistance cluster, the activity of the host D-Ala : D-Ala ligase is not required because the bacteria make use of an alternative ligase involved in resistance (VanA or VanB) that ligates D-Lac to D-Ala. Thus, the ddl gene encoding the host D-Ala : D-Ala ligase accumulates mutations and bacteria can only grow in the presence of the antibiotic because it induces expression of the alternative, antibiotic-resistance-linked ligase (Baptista et al, 1997;Sifaoui & Gutmann, 1997;Van Bambeke et al, 1999).…”

Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

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A global view of antibiotic resistance

et al. 2009

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Antibiotic resistance is one of the few examples of evolution that can be addressed experimentally. The present review analyses this resistance, focusing on the networks that regulate its acquisition and its effect on bacterial physiology. It is widely accepted that antibiotics and antibiotic resistance genes play fundamental ecological roles - as weapons and shields, respectively - in shaping the structures of microbial communities. Although this Darwinian view of the role of antibiotics is still valid, recent work indicates that antibiotics and resistance mechanisms may play other ecological roles and strongly influence bacterial physiology. The expression of antibiotic resistance determinants must therefore be tightly regulated and their activity forms part of global metabolic networks. In addition, certain bacterial modes of life can trigger transient phenotypic antibiotic resistance under some circumstances. Understanding resistance thus requires the analysis of the regulatory networks controlling bacterial evolvability, the physiological webs affected and the metabolic rewiring it incurs.

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Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

Section: Antibiotic-dependent Bacterial Growthmentioning

confidence: 99%

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A global view of antibiotic resistance

et al. 2009

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“…1994; Farrag et al. 1996) and Enterococcus avium (Sifaoui and Gutmann 1997) with the vanA gene have also been described. Vancomycin‐dependence results from a mutation that inactivates the d ‐Ala : d ‐Ala ligase gene ( ddl ) in the chromosome, so that the mutant strain no longer produces d ‐Ala : d ‐Ala‐ending peptidoglycan precursors.…”

Section: Introductionmentioning

confidence: 99%

A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China

Tanimoto

Nomura

Hamatani

et al. 2005

Lett Appl Microbiol

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Aims: The characterization of KC122.1, which is a vancomycin‐dependent VRE (Vancomycin‐resistant enterococci) (Enterococcus faecalis) and the first case in Japan of a VRE isolate obtained from chicken meat imported from China. Methods and Results: PCR amplification of vanA, vanS and ddl gene and direct sequencing of the PCR products were performed. KC122.1 was a VanA‐type VRE showing high‐level vancomycin resistance and low‐level teicoplanin resistance, and its vanS gene had three point mutations. The ddl gene of KC122.1 was sequenced and two changes were found at the ninth codon (GCC–GAC) and the stop codon (TAA–CAA). The latter change was also found in the laboratory strain E. faecalis FA2‐2. Conclusions: Three point mutations in vanS resulted in high‐level vancomycin resistance and low‐level teicoplanin resistance. The change at the ninth codon resulted in the inactivation of the ddl gene and vancomycin‐dependent growth. An eight amino acid extension at the C‐terminal did not impair the function of the d‐Ala : d‐Ala ligase. Significance and Impact of the study: This is the first example of the isolation of VRE from chicken meat imported from China and the first vancomycin‐dependent VRE from a nonhuman source.

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“…Enterococci with impaired Ddl activity that require the presence of a glycopeptide for growth have been reported (6,19,21,24). Although N97-330 appears to have a nonfunctional Ddl due to insertion of an IS element, a glycopeptide is not required for growth due to a frameshift mutation in vanS D , which most likely leads to the constitutive expression of the van operon.…”

mentioning

confidence: 99%

Molecular Characterization of the vanDGene Cluster and a Novel Insertion Element in a Vancomycin-Resistant Enterococcus Isolated in Canada

Boyd

Conly

Dedier

et al. 2000

J. Clin. Microbiol.

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A single vanD-containing Enterococcus faecium strain (N97-330) was isolated in Canada. The vanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanD region in BM4339, the vanS D gene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddl gene revealed the presence of an IS982-like element (ISEfm1) which interrupted the D-Ala-D-Ala ligase. This suggested the constitutive expression of the vanD operon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element.

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Vancomycin dependence in a vanA-producing Enterococcus avium strain with a nonsense mutation in the natural D-Ala-D-Ala ligase gene

Cited by 32 publications

References 6 publications

A global view of antibiotic resistance

A global view of antibiotic resistance

A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China

Molecular Characterization of the vanDGene Cluster and a Novel Insertion Element in a Vancomycin-Resistant Enterococcus Isolated in Canada

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