A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China

Tanimoto, Koichi; Nomura, Takahiro; Hamatani, Hiroko; Xiao, Yong Hong; Ike, Yasuyoshi

doi:10.1111/j.1472-765x.2005.01722.x

Cited by 16 publications

(20 citation statements)

References 21 publications

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“…Our group reported the first case of VanA-type Enterococcus faecalis: strain KC122.1, isolated in Japan from chicken meat imported from mainland China in 2001 (25). The Tn1546-like element was encoded on a conjugative plasmid and had three specific substitutions of VanS, resulting in lowlevel teicoplanin resistance, as mentioned above.…”

Section: Resultsmentioning

confidence: 99%

“…In the meantime, the current recommended hospital infection control measures for developed countries may be readily implemented to prevent further spread of VRE in mainland China. KC122.1 is a VanA-type E. faecalis strain isolated from chicken meat imported from mainland China (25). The Tn1546-like element of KC122.1 was temporarily classified as Thai-type, which is often found in VRE isolates from chicken meats imported from Thailand (9,18 …”

Section: Resultsmentioning

confidence: 99%

“…Thirteen clinical isolates of vancomycinresistant E. faecium recovered from blood cultures and urine and sputum samples from patients in China were used in this study (Table.1). VanA-type vancomycin-resistant Enterococcus faecalis strain KC122.1, isolated in Japan from chicken meat imported from China in 2001, was used for the comparative analysis of Tn1546-like elements (25). E. faecium strains BM4105RF and BM4105SS were used as recipient strains for transfer experiments (13).…”

Section: Methodsmentioning

confidence: 99%

“…Our group has previously described vancomycin-dependent VanA-type VRE strains isolated in Japan from retail chicken meat imported from China (25). Clinical isolates of VanA-type VRE (E. faecium) are rarely obtainable from China.…”

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confidence: 99%

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Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Mainland China

Zheng

Tomita²,

Xiao

et al. 2007

J Clin Microbiol

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Little is known about vancomycin-resistant enterococci in China. Thirteen pulsed-field gel electrophoresisconfirmed heterogeneous VanA-type vancomycin-resistant Enterococcus faecium (VRE) isolates were obtained from five Chinese hospitals from 2001 to 2005. The isolates were typed by multilocus sequence typing into nine different sequence types (STs), including five new STs (ST18, ST25, ST78, ST203, ST320, ST321, ST322, ST323, and ST335). Vancomycin resistance in each isolate was encoded on conjugative plasmids; two of the plasmids, pZB18 (67 kbp) and pZB22 (200 kbp), were highly conjugative and were able to transfer at high frequencies of around 10 ؊4 and 10 ؊7 per donor cell in broth mating, respectively. None of the plasmids identified in these isolates carried traA, which is usually conserved in the pMG1-like highly conjugative plasmid for E. faecium, implying that pZB18 and pZB22 were novel types of a highly conjugative plasmid in enterococci. Thirteen Tn1546-like elements encoding VanA-type VRE on the conjugative plasmids were classified into six types (types I to VI), and most of them contained both IS1216V and IS1542 insertions. The isolates carrying the type II element were predominant. The six type elements were different from that of a VanA-type Enterococcus faecalis strain isolated from Chinese chicken meat. The results suggested that the disseminations of VRE in these areas were by Tn1546-like elements being acquired by the conjugative plasmids and transferred among E. faecium strains.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Mainland China

Zheng

Tomita²,

Xiao

et al. 2007

J Clin Microbiol

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“…More than 10 years ago, we isolated GRE strains from both imported and domestic meats, including chicken meat (9). Since then, we have examined both imported and domestic meat samples as part of a surveillance program looking at GRE contamination (16,19). While VanAand VanB-type GRE strains, which show high-level resistance to glycopeptides, are occasionally detected in the samples, most of the GRE isolates from the meat samples had VanC-type resistance, which is carried naturally by enterococci that show low-level resistance to glycopeptides.…”

mentioning

confidence: 99%

Identification of VanN-Type Vancomycin Resistance in an Enterococcus faecium Isolate from Chicken Meat in Japan

Nomura

Tanimoto

Shibayama

et al. 2012

Antimicrob Agents Chemother

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e Five VanN-type vancomycin-resistant Enterococcus faecium strains were isolated from a sample of domestic chicken meat in Japan. All isolates showed low-level resistance to vancomycin (MIC, 12 mg/liter) and had the same pulsed-field gel electrophoresis profile. The vancomycin resistance was encoded on a large plasmid (160 kbp) and was expressed constitutively. The VanN-type resistance operon was identical to the first resistance operon to be reported, with the exception of a 1-bp deletion in vanT N and a 1-bp substitution in vanS N . Since the first reports of vancomycin resistance in Enterococcus faecium in 1988 (12, 20), the glycopeptide-resistant enterococci (GRE) have become increasingly widespread throughout the world and are found as multiresistant opportunistic pathogens in hospitals and also in the environment (food animals). To date, nine types of operon structure conferring resistance to glycopeptides have been reported (5, 11). , vanC2, vanC3, vanE, vanG, vanL In Japan, there is a lower incidence of GRE in humans and animals than in other countries (16). However, there are several reports showing the possible transmission of GRE or glycopeptide resistance between humans and food animals through food products such as chicken meat (8, 16). More than 10 years ago, we isolated GRE strains from both imported and domestic meats, including chicken meat (9). Since then, we have examined both imported and domestic meat samples as part of a surveillance program looking at GRE contamination (16,19). While VanAand VanB-type GRE strains, which show high-level resistance to glycopeptides, are occasionally detected in the samples, most of the GRE isolates from the meat samples had VanC-type resistance, which is carried naturally by enterococci that show low-level resistance to glycopeptides. We recently identified VanN-type GRE strains isolated from a sample of domestic chicken meat. Here we present the results of the analysis of those strains.During the period from February to May 2011, a total of 322 meat and swab samples from meat destined for consumption in Japan were collected and investigated. The samples were obtained from two major national quarantine stations (Yokohama and Kobe) and from three meat inspection offices (Gunma, Kagoshima, and Miyazaki) in Japan. They included samples from 90 domestic chickens, 45 domestic pork meat samples, 85 imported chickens, and 102 imported pork meat samples. The country or region of origin of each sample is listed in Table 1. A hundred grams of each meat sample (mincemeat) was smashed and homogenized using an EXNIZER 400 (Organo, Japan) in 150 ml of buffered peptone water (Nissui, Japan). Eight milliliters of the supernatant from the homogenized meat sample was mixed with 32 ml of esculin saline buffer containing vancomycin at a concentration of 6 mg/liter and preincubated for 48 h at 37°C. After preincubation, 0.1 ml of each culture broth was spread on bile esculin azide agar (BEAA) plates containing vancomycin at a concentration of 6 mg/liter. The meat swab samples ...

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Antibiotic Resistance in Enterococci

Ghosh¹,

Zurek²

2015

Antimicrobial Resistance and Food Safety

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A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China

Cited by 16 publications

References 21 publications

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Mainland China

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Mainland China

Identification of VanN-Type Vancomycin Resistance in an Enterococcus faecium Isolate from Chicken Meat in Japan

Antibiotic Resistance in Enterococci

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