Value of galactomannan detection by sandwich enzyme-linked immunosorbent assay in the early diagnosis and follow-up of invasive aspergillosis

Strong Correlation between Serum Aspergillus Galactomannan Index and Outcome of Aspergillosis in Patients with Hematological Cancer: Clinical and Research Implications

“…P values р.05 were considered to be k p 0.4 statistically significant The KCC was determined for all eligible patients and for patients included in prospective studies only, with further analysis by age group (adults only vs. adults plus pediatric patients). Three prospective studies focused exclusively on pediatric patients [23,24,30], but only 1 had the data required for this study.…”

“…Because many studies did not include autopsy examination and because of decreasing rates of performance of autopsy [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29], we evaluated survival (alive vs. dead) without taking into consideration autopsy finding or causes of death (aspergillosis vs. other), as reported by the original authors.…”

Aspergillus fumigatusand Aspergillosis

“…3 for the detection of GM is currently the most sensitive method developed (628). Several studies performed in Europe have shown that the sandwich ELISA contributes to the early diagnosis of IA, and the inter-and intralaboratory reproducibility of the method is reasonably good (74,395,558,628,630,638,683,685). In contrast to previous reports, GM was detected in all specimens following the first positive specimen during the course of disease in a given patient.…”

“…Although it is known that the highest concentration of GM is always released in the terminal phases of the disease, the pharmacokinetics of the antigen in infected animals or humans has been insufficiently studied (47). Depending upon the patient, positive antigenemia can last from 1 week to 2 months (74,558,630,638,683). GM is detected at a lower concentration in urine, (0.5 ng of GM/ml) than in serum (1 ng of GM/ml) (628).…”

Serologische Untersuchungen in der Aspergillus-Diagnostik

“…zuru È ckgehen (Bodey et al, 1993) und Anfang der neunziger Jahre wurde u È ber eine Verdopplung disseminierter Mykosen bei Autopsien innerhalb von zehn Jahren berichtet, wobei invasive Aspergillosen von 17 auf 60 % anstiegen (Groll et al, 1993 Unter diesen Bedingungen wurde von den Autoren eine Sensitivita È t von 90 % und eine Spezifita È t von 84 % fu È r den Galaktomannan-Nachweis mittels ELISA-Test ermittelt. Tabone et al (1996) untersuchten 76 Kinder mit Immundefiziten, von denen 8 eine invasive Aspergillose entwickelten. Fu È r den in dieser Studie verwendeten ELISA-Test auf Galaktomannan wird von den Autoren eine Sensitivita È t von 100 %, eine Spezifita È t von 89 %, ein positiver pra È diktiver Wert von 53 % und ein negativer pra È diktiver Wert von 100 % angegeben.…”