Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154

Aachary, Ayyappan Appukuttan; Prapulla, S. G.

doi:10.1016/j.biortech.2008.06.050

Cited by 147 publications

(53 citation statements)

References 19 publications

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“…The addition of XOS to food has excellent physiological eff ects on the organism, including improvement of bowel function, calcium absorption, prevention of dental caries, protection against cardiovascular disease and reduction of colon cancer risks due to the formation of smaller chain fatt y acids (8,9). In addition, they contribute to benefi cial eff ects related to skin and blood, immunological action, antioxidant activities, anti-infl ammatory and antiallergenic eff ects (1,10).…”

Section: Introductionmentioning

confidence: 99%

“…High quality XOS can be produced enzymatically using xylanases from a variety of microorganisms including: Aspergillus, Thermoascus, Trichoderma, Streptomycetes, Phanerochaetes, Chytridiomycetes, Ruminoccocus, Penicillium, Fibrobacter, Clostridium, Pichia and Bacillus (1,(10)(11)(12)(13). However, the search for more effi cient xylanase-producing strains is necessary considering the production costs and low yields of production.…”

Section: Introductionmentioning

confidence: 99%

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Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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Currently, lignocellulosic waste is a topic of global studies, given that fossil fuel reserves are diminishing, the new agricultural frontiers are limited and the demand for food and biofuels is increasing by the growing world population (1). For these reasons, technology must advance to improve the use of agricultural and agroindustrial residues to obtain food and biofuel.Xylans present in lignocellulosic materials have been studied for obtaining xylooligosaccharides (XOS) from waste materials such as corncobs, rice hulls, olive pits, barley straw (2), tobacco stalk, cott on stalk, sunfl ower stalk, wheat straw (3) and sugarcane bagasse (4-6). Sugarcane bagasse is inexpensive, renewable and abundant source of XOS especially in the countries that produce ethanol and sugar from sugarcane. However, more research is necessary to improve the use of this residue. SummarySugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (XOS). Some XOS are considered soluble dietary fi bre, with low caloric value and prebiotic eff ect, but they are expensive and not easily available. In a screening of 138 fungi, only nine were shortlisted, and just Aspergillus fumigatus M51 (35.6 U/mL) and A. fumigatus U2370 (28.5 U/mL) were selected as the most signifi cant producers of xylanases. These fungi had low β-xylosidase activity, which is desirable for the production of XOS. The xylanases from Trichoderma reesei CCT 2768, A. fumigatus M51 and A. fumigatus U2370 gave a signifi cantly higher XOS yield, 11.9, 14.7 and 7.9 % respectively, in a 3-hour reaction with hemicellulose from sugarcane bagasse. These enzymes are relatively thermostable at 40-50 °C and can be used in a wide range of pH values. Furthermore, these xylanases produced more prebiotic XOS (xylobiose and xylotriose) when compared with a commercial xylanase. The xylanases from A. fumigatus M51 reached a high level of XOS production (37.6 %) in 48-72 h using hemicellulose extracted from sugarcane bagasse. This yield represents 68.8 kg of prebiotic XOS per metric tonne of cane bagasse. In addition, in a biorefi nery, aft er hemicellulose extraction for XOS production, the residual cellulose could be used for the production of second-generation ethanol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Carvalho

Neto

Almeida

et al. 2015

Food Technol. Biotechnol.

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“…Thus, the presence of branch points in the xylan chain seems to prevent the complete hydrolysis of xylans by crude filtrate of T. aurantiacus. The effect of enzyme concentration on XOS production was also studied in same range to this work to hydrolysis of alkali pretreated corncob powder [23]. The amount of XOS produced was around 5.9 mg/mL in the lowest enzyme charge, which increased to a value of 10.62 mg/ mL at an enzyme concentration of 14 U/mL, and thereafter, the increase was very insignificant with respect to increase in enzyme concentration.…”

Section: Fitting Model With Response Surface Methodsmentioning

confidence: 95%

Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus

Brienzo

Carvalho

Milagres

2010

Appl Biochem Biotechnol

137

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Sugarcane bagasse hemicellulose was isolated in a one-step chemical extraction using hydrogen peroxide in alkaline media. The polysaccharide containing 80.9% xylose and small amounts of L-arabinose, 4-O-methyl-D-glucuronic acid and glucose, was hydrolyzed by crude enzymatic extracts from Thermoascus aurantiacus at 50 degrees C. Conditions of enzymatic hydrolysis leading to the best yields of xylose and xylooligosaccharides (DP 2-5) were investigated using substrate concentration in the range 0.5-3.5% (w/v), enzyme load 40-80 U/g of the substrate, and reaction time from 3 to 96 h, applying a 2(2) factorial design. The maximum conversion to xylooligosaccharides (37.1%) was obtained with 2.6% of substrate and xylanase load of 60 U/g. The predicted maximum yield of xylobiose by a polynomial model was 41.6%. Crude enzymatic extract of T. aurantiacus generate from sugarcane bagasse hemicellulose 39% of xylose, 59% of xylobiose, and 2% of other xylooligosaccharides.

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“…Xylose oligomers can be produced from xylan either by chemical or enzymatic hydrolysis or by a combination of both [1,2,17,22,26]. Xylose oligomers have been reported to have a prebiotic effect on humans [17,23] and inhibit the growth of pathogens in human intestines [23].…”

Section: Introductionmentioning

confidence: 99%

Separation and purification of xylose oligomers using centrifugal partition chromatography

Lau

Bunnell

Clausen

et al. 2010

J Ind Microbiol Biotechnol

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Xylose oligomers, which have a prebiotic effect, have been used as additives to human and animal food. These oligomers are also the primary intermediate in hemicellulose degradation during the pretreatment of biomass. Centrifugal partition chromatography (CPC) was used in this study to separate and purify xylan-derived oligomers from birchwood xylan. The xylan was partially hydrolyzed to achieve varying degrees of polymerization at 130°C using 0.98% aqueous sulfuric acid for 20 min with a 2.5% solid loading. The CPC solvent system consisting of dimethyl sulfoxide (DMSO), tetrahydrofuran (THF), and water in a 1:6:3 volumetric ratio was used because of its ability to dissolve xylose oligomers of different degrees of polymerization. The CPC was operated in the ascending mode with the water- and DMSO-rich bottom phase acting as the stationary phase, while the THF-rich top phase was the eluent. This paper delineates a method for the production and purification of xylose monomer and xylose oligomers (up to xylopentaose) using CPC. The amount and purity of compounds collected from the CPC fractionation based on 1 g of birchwood xylan were 25.26 mg of xylose at 91.86% purity, 10.71 mg of xylobiose at 85.07% purity, 4.15 mg of xylotriose at 54.71% purity, 5.03 mg of xylotetraose at 38.33% purity and 3.31 mg of xylopentaose at 30.43% purity.

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Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154

Cited by 147 publications

References 19 publications

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Screening of Xylanolytic Aspergillus fumigatus for Prebiotic Xylooligosaccharide Production Using Bagasse

Xylooligosaccharides Production from Alkali-Pretreated Sugarcane Bagasse Using Xylanases from Thermoascus aurantiacus

Separation and purification of xylose oligomers using centrifugal partition chromatography

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