Separation and purification of xylose oligomers using centrifugal partition chromatography

Abstract

Xylose oligomers, which have a prebiotic effect, have been used as additives to human and animal food. These oligomers are also the primary intermediate in hemicellulose degradation during the pretreatment of biomass. Centrifugal partition chromatography (CPC) was used in this study to separate and purify xylan-derived oligomers from birchwood xylan. The xylan was partially hydrolyzed to achieve varying degrees of polymerization at 130°C using 0.98% aqueous sulfuric acid for 20 min with a 2.5% solid loading. The CPC solvent system consisting of dimethyl sulfoxide (DMSO), tetrahydrofuran (THF), and water in a 1:6:3 volumetric ratio was used because of its ability to dissolve xylose oligomers of different degrees of polymerization. The CPC was operated in the ascending mode with the water- and DMSO-rich bottom phase acting as the stationary phase, while the THF-rich top phase was the eluent. This paper delineates a method for the production and purification of xylose monomer and xylose oligomers (up to xylopentaose) using CPC. The amount and purity of compounds collected from the CPC fractionation based on 1 g of birchwood xylan were 25.26 mg of xylose at 91.86% purity, 10.71 mg of xylobiose at 85.07% purity, 4.15 mg of xylotriose at 54.71% purity, 5.03 mg of xylotetraose at 38.33% purity and 3.31 mg of xylopentaose at 30.43% purity.

Xylooligosaccharides Production, Quantification, and Characterization in Context of Lignocellulosic Biomass Pretreatment

“…In addition, samples can be recovered by flushing the system since the stationary phase is also a liquid. In contrast to other chromatographic techniques such as GPC, CPC could be used for preparative separation or purification because of its large stationary phase volume [75].…”

“…CPC has been widely used as an efficient purification and separation tool for many compounds including flavonoids, flavonolignans, and macrolide antibiotics [75]. Shibusawa et al [76] employed CPC to purify apple-derived catechin oligosaccharides by operating in an ascending mode with a solvent system containing equal volumes of hexane, methyl acetate, acetonitrile, and water.…”

“…Lau et al [75] used CPC to separate and purify xylan-derived oligosaccharides from birchwood xylan. A CPC solvent system containing dimethyl sulfoxide (DMSO), tetrahydrofuran (THF), and water in a 1:6:3 volumetric ratio, respectively, was chosen for its ability to dissolve XOs of different DPs.…”

Separation of xylose oligomers from autohydrolyzed Miscanthus×giganteus using centrifugal partition chromatography

“…Advantages of CPC include the ability to separate compounds that span a wide molecular weight range, such as XOS (Berthod et al, 1988). Birchwood xylan-derived XOS were previously fractionated by CPC into its individual oligomers with a tetrahydrofuran-based solvent system (Lau et al, 2011). The CPC method was further improved with a butanol-based solvent system (Lau et al, 2013).…”

“…The objective of this study was to purify XOS from crude MxG hydrolyzates, which were produced through autohydrolysis, into their individual oligomer components. It is important to note that the starting material in this study consisted of a crude complex hydrolyzate, as opposed to a semi purified xylan material (Lau et al, 2011;Lau et al, 2013;Bunnell et al, 2015).…”