Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast

Rywińska, Anita; Rymowicz, Waldemar; Marcinkiewicz, Marta

doi:10.2225/vol13-issue4-fulltext-1

Cited by 44 publications

(34 citation statements)

References 31 publications

(23 reference statements)

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“…Examples of such applications include but are not limited to the use of crude glycerol in animal feeds both for ruminants and non ruminants animals [46]. Feed stock for fermentative production 1,3-propanediol by Klebsiella pneumonia [47], biosynthesis of citric acid from crude glycerol by Yarrowia lipolytica ACA-DC 50109 [46] and fermentative conversion of crude glycerol to hydrogen by the bacterium Rhodopseudomonas palustris [48,49,50]. Glycerol can also be a good source of various solvents such as propylene glycols, glycerol ethers and esters.…”

Section: Dehydrationmentioning

confidence: 99%

Castor oil as a potential renewable resource for the production of functional materials

Mubofu

2016

Sustain Chem Process

170

124

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Castor oil is increasingly becoming an important bio-based raw material for industrial applications. The oil is nonedible and can be extracted from castor seeds from the castor plant belonging to the family Euphorbiaceae. The oil is a mixture of saturated and unsaturated fatty acid esters linked to a glycerol. The presence of hydroxyl group, a double bond, carboxylic group and a long chain hydrocarbon in ricinoleic acid (a major component of the oil), offer several possibilities of transforming it into variety of materials. The oil is thus a potential alternative to petroleum-based starting chemicals for the production of materials with variety of properties. Despite this huge potential, very little has recently been reviewed on the use of castor oil as a bio-resource in the production of functional materials. This review therefore highlights the potential of castor oil in the production of these diverse materials with their projected global market potential. The review gives the background information of castor oil and its geographical availability, the properties and its uses as bio-based resource for synthesis of various materials. The review further highlights on the use of castor oil or ricinoleic acid as a green capping agent in the synthesis of nanomaterials.

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Section: Dehydrationmentioning

confidence: 99%

Castor oil as a potential renewable resource for the production of functional materials

Mubofu

2016

Sustain Chem Process

170

124

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“…When the studies in the literature are reviewed, it is seen that in general, HPLC is used for determining the amount of organic acids [5,6,18,19]. However, the amounts of CA and ICA cannot be determined separately with chromatographic methods; thus, the amount of ICA is determined with the enzymatic kit.…”

Section: Discussionmentioning

confidence: 99%

“…Karasu [21] investigated various parameters (carbon source, pH, temperature, some minerals) that affect the production of citric acid with Yarrowia lipolytica and evaluated the citric acid production capacity of some of the endogenous yeast strains. It has been observed that studies in the world mostly concentrate on the improvement of current strains as higher productive agents through mutations or on the realization of higher citric acid production by supporting the production with different methods rather than on the screening of higher citric acid-producing strains [19,[23][24][25]. In Turkey, however, screening of high citric acid producing strains and the development of these strains still are of importance.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, glycerol has been successfully used as a carbon source in the production of citric acid with Y. lipolytica [6,18,19,26,27]. In the literature, it has been reported that in addition to studies in which pure glycerol is used as a substrate, there are other studies in which crude glycerol is used as a byproduct in the production of biodiesel and soap [2,3,19,22,28].…”

Section: Discussionmentioning

confidence: 99%

“…In the literature, it has been reported that in addition to studies in which pure glycerol is used as a substrate, there are other studies in which crude glycerol is used as a byproduct in the production of biodiesel and soap [2,3,19,22,28].…”

Section: Discussionmentioning

confidence: 99%

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Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Uçar¹,

Çelik²,

Akpınar³

et al. 2014

Turk J Bioch

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Objective: The goal of this study was to determine the influence of various carbon sources on the citric and isocitric acid production by various Yarrowia lipolytica strains. Methods:The yeasts used in our study were first investigated for organic acid production using screening media. Then, the effect of several complex carbon sources on the citric and isocitric acid production of selected yeast strains was investigated. The amount of citric and isocitric acid production was determined via enzymatic reactions. Results: In this study, 22 Y. lipolytica strains were investigated for the organic acid production. Among these strains, 2 strains (TEM YL 3 and TEM YL 20) were found to be highest organicacid producer. Taken all results together, the highest amounts of citric acid (66.2 g/L for TEM YL 3, 50.0 g/L for TEM YL 20) were observed in the production medium containing sunflower oil. Conclusion: Citric acid consumption, and thus, the need for it are constantly rising in our country, which imports citric acid. Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains. Key Words: Organic acid production, citric acid, isocitric acid, Yarrowia lipolytica. Conflict of Interest: Authors do not have any conflict of interest. ÖZETAmaç: Bu çalışmada, Yarrowia lipolytica suşları tarafından sitrik ve izositrik asit üretimi üzerine çeşitli karbon kaynaklarının etkisinin saptanması amaçlanmıştır. Metod: İlk olarak çalışmamızda kullandığımız mayalar, organik asit üretimleri açısından tarama besiyerlerinde test edilmişlerdir. Daha sonra, bu maya strainleri tarafından üretilen sitrik ve izositrik asit üretimi üzerine farklı karbon kaynaklarının etkisi araştırılmıştır. Sitrik ve izositrik asit üretim miktarları enzimatik test kitleri kullanılarak saptanmıştır. Bulgular: Bu çalışmada, organik asit üretimi açısından 22 Y. lipolytica suşu incelenmiştir. Bu 22 maya suşu arasından iki suşun (TEM YL 3 ve TEM YL 20), en yüksek organik asit üretici maya suşları olduğu bulunmuştur. Bütün sonuçlar değerlendirildiğinde, en yüksek sitrik asit üretimi (TEM YL 3 için 66.2 g/L, TEM YL 20 için 50.0 g/L) ayçiçek yağı içeren üretim ortamında saptanmıştır. Sonuç: Özellikle sitrik asit ithalatı yapılan ülkemizde, sitrik asit tüketimi ve ona olan ihtiyaç sürekli olarak artmaktadır. Atık karbon kaynaklarının değerlendirilmesi ve maliyeti düşük ancak verimi yüksek sitrik asit üreten yeni suşların kullanılarak, maksimum sitrik asit verimini sağlayacak daha ileri çalışmalara ihtiyaç duyulmaktadır. Material and Methods Microorganisms and growth conditionsDomestic Y. lipolytica strains TEM YL 3 and TEM YL 20 had been isolated from cheese [14] and identified with phenotypic and genotypic approach [14,15]. Initially yeast cells were grown aerobically at 27°C in a liquid YPD medium including (1.0 % yeast extract, 2.0 % peptone, and 2.0 % glucose). Screening of total organic acid pro...

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Conversion of biodiesel‐derived glycerol into biotechnological products of industrial significance by yeast and fungal strains

Papanikolaou

Rontou

Belka

et al. 2016

Engineering in Life Sciences

101

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Oleochemical activities (e.g. biodiesel production, fat saponification) generate annually very high amounts of concentrated glycerol‐containing waters (called crude glycerol) as the principal residues of these processes. Crude glycerol is an industrial residue the valorization of which attracts remarkable and constantly increasing interest. In the current investigation, biodiesel‐derived glycerol was employed as substrate for yeast and fungal strains cultivated under nitrogen‐limited conditions in shake flasks. Glucose was employed as reference substrate. Several yeasts (Candida diddensiae, Candida tropicalis, Pichia ciferrii, Williopsis saturnus, Candida boidinii, and Candida oleophila) rapidly assimilated glucose and converted it into ethanol, despite aerobic conditions imposed, and were Crabtree‐positive. None of these yeasts produced ethanol during growth on glycerol or accumulated significant quantities of lipid during growth on glucose or glycerol. Only Rhodosporidium toruloides produced notable lipid quantities from glucose and to lesser extent from glycerol. Yarrowia lipolytica LFMB 20 produced citrate ≈58 g/L growing on high‐glucose media, while on high‐glycerol media ≈42 g/L citrate and ≈18 g/L mannitol. During growth on glucose/glycerol blends, glycerol was assimilated first and thereafter glucose was consumed. Fungi produced higher lipid quantities compared with yeasts. High lipid quantities were produced by Mortierella ramanniana, Mucor sp., and mainly Mortierella isabellina, with glycerol being more adequate for M. ramanniana and glucose for Mucor sp. and M. isabellina. M. isabellina ATHUM 2935 produced lipids of 8.5 g/L, 83.3% w/w in dry cell weight (DCW) and conversion yield per unit of glucose consumed ≈0.25 g/g. The respective values on glycerol were 5.4 g/L, 66.6% w/w in DCW and ≈0.22 g/g. Lipids of all microorganisms were analyzed with regards to their fatty acid composition, and M. isabellina presented the closest similitude with rapeseed oil. Crude lipids produced by this fungus and extracted with chloroform/methanol blend, were composed mostly of triacylglycerols, thus indicating that these solvents are adequate for triacylglycerol extraction.

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Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast

Cited by 44 publications

References 31 publications

Castor oil as a potential renewable resource for the production of functional materials

Castor oil as a potential renewable resource for the production of functional materials

Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Conversion of biodiesel‐derived glycerol into biotechnological products of industrial significance by yeast and fungal strains

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