Valorization of antioxidants extracted from olive mill wastewater

Abstract

Antioxidants are highly important gradients used to preserve cosmetic products and reduce the effect of oxidative stress on the skin. The present work explores the possibility of using phenolic compounds of olive mill wastewater (OMW) as effective alternatives to the commercial antioxidants used in cosmetic formulations deemed by their allergic and carcinogenic effects. Esterification of tyrosol and hydroxytyrosol extracted from OMW with various fatty acids was conducted using Novozyme 435 lipase as a biocatalyst. Upon synthesis, butyrate, caprate, laurate, and palmitate tyrosyl and hydroxytyrosyl esters were isolated and evaluated for their antioxidant and antibacterial activities. Results showed that laurate derivatives are the most efficient in preventing lipid oxidation and inhibiting growth of pathogenic strains. In the prospective of industrial use, laurate tyrosyl and hydroxytyrosyl derivatives were incorporated in a formulation of moisturizer to substitute the commercial antioxidant butylated hydroxyltoluene. Oleuropein, extracted from olive leaves powder, was also tested as an antiaging ingredient in cosmetic formulations. The evaluation of physicochemical, microbiological, and sensorial properties of the new cosmetic products indicated that oleuropein and lipophilic derivatives do not affect the properties of the standard formulation. Oleuropein and lipophilic derivatives can be added as active ingredients to stabilize cosmetic preparations.

Olive mill wastewater as a potential source of antibacterial and anti-inflammatory compounds against the food-borne pathogen Campylobacter

“…These results demonstrate the strong capacity of the fraction F5 to inhibit Campylobacter growth. According to the described results, recent studies have also demonstrated the antibacterial effects of phenolic and secoiridoid enriched fractions obtained from OMW on several bacterial pathogens, including gram positive and gram negative bacteria (Aissa et al, 2017;Belaqziz et al, 2017). Usually, the antibacterial effect is greater in grampositive than gram-negative bacteria, which is consistent with a thicker and more complex cell wall in the case of gram-negative bacteria (Leouifoudi et al, 2015;Obied et al, 2007).…”

Novozym 435: the “perfect” lipase immobilized biocatalyst?

“…For example, es-terification of tyrosol and hydroxytyrosol extracted from olive mill wastewater with various fatty acids (caprate, laurate, and palmitate) catalyzed by N435 was successfully performed and shown to be efficient to avoid lipid oxidation. 434 3.2.4. Interesterification of glycerides.…”

Valorisation of olive mill wastewater by phenolic compounds adsorption: Development and application of a procedure for adsorbent selection

“…The detected PCs are generally recognized as potent antioxidants [59], antibacterial (gram positive and gram negative), antifungal, and have demonstrated activities against yeast [60]. Their industrial application for these properties are increasing, both in the food industry, as reported for hydroxytyrosol and verbascoside in meat preparation [59,61], or olive leaf phenolics in dairy preparation [62] and OMW extract for olive oil preservation [60], as well as in the cosmetic industries, as reported for oleuropein used as antiaging ingredient in cosmetic formulations [63].…”