Valorization of agro-starchy wastes as substrates for oleaginous microbes

Chaturvedi, Shivani; Bhattacharya, Amrik; Nain, Lata; Prasanna, Radha; Khare, Sunil Kumar

doi:10.1016/j.biombioe.2019.105294

Cited by 34 publications

(13 citation statements)

References 52 publications

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“…| © 2020 Wiley-VCH GmbH agro-waste as a substrate [10,11]. The yeast strains grew robustly on the starchy wastes [12], agro and industrial processing wastes (peel of a banana, potato, yam, cassava and residues of rice, corn, wheat, barley) without any pretreatment, indicating the usefulness of these substrates for the lipid production [2,13,14]. Tanimura et al [15] utilized starch for lipid production (61.96% lipid content, 3.02 g/L lipid output) by Cryptococcus terricola JCM 2452.…”

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confidence: 99%

Overexpression and repression of key rate‐limiting enzymes (acetyl CoA carboxylase and HMG reductase) to enhance fatty acid production from Rhodotorula mucilaginosa

et al. 2020

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Implementing two‐way strategies to enhance the lipid production in Rhodotorula mucilaginosa with the help of metabolic engineering was focused on the overexpression of acetyl coenzyme A carboxylase (ACC1 carboxylase) gene and repression of 3‐hydroxy 3‐methylglutaryl reductase (HMG‐CoA reductase). Using an inducer (sodium citrate) and inhibitor (rosuvastatin), the amounts of biomass, lipid, and carotenoid were estimated. In the presence of inhibitor (200 mM), 62% higher lipid concentration was observed, while 44% enhancement was recorded when inducer (3 mM) was used. A combination of both inhibitor and inducer resulted in a 57% increase in lipid concentration by the oleaginous yeast. These results were again confirmed by real‐time polymerase chain reaction by targeting the expression of the genes coding for ACC1 carboxylase and 13‐fold increase was recorded in the presence of inducer as compared with control. This combined strategy (inducer and inhibitor use) has been reported for the first time as far as the best of our knowledge. The metabolic engineering strategies reported here will be a powerful approach for the enhanced commercial production of lipids.

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Overexpression and repression of key rate‐limiting enzymes (acetyl CoA carboxylase and HMG reductase) to enhance fatty acid production from Rhodotorula mucilaginosa

et al. 2020

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“…The different plants generate different kinds of byproducts; for example, non-woody vegetables generate residual and structural fractions that can be utilized for the preparation of various biobased products. Similarly, the agroindustrial byproducts can be used as a raw material in biorefineries for the release of sugars, proteins, oils and other micronutrients, which can be employed for the development of different media compositions (Ascencio et al, 2019;Chaturvedi et al, 2019). Among some successful biorefineries, we can highlight Abengoa, Beta Renewables, DuPont, GranBio, Poet-DSM, and Raizen, which operate on the commercial scale by using lignocellulosic feedstocks and different pretreatment processes.…”

Section: Agroindustrial Byproductsmentioning

confidence: 99%

“…The starchy biomass is majorly composed of starch, which is generally present in seeds and tubers, and its constituents are amylose and amylopectin (Chaturvedi et al, 2019). Grains like barley, corn, rice, soybean, and wheat are composed of three fractions: endosperm (presenting the starchy portion, responsible for seed maintenance during the dormant phase and before germination), germ (presenting the vitamins, proteins, minerals, and oils, responsible for the development of the plant), and bran (the external layer that covers the grain, protecting the endosperm and germ against external agents) (Barrett et al, 2020).…”

Section: Starchy Byproductmentioning

confidence: 99%

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

et al. 2020

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The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

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“…Wild et al [18], reported higher Lipomyces starkeyi lipids on the starch substrate (rice residue) than glucose, since the strain is capable of directly converting ASY2 in SWW ASY2 in SWW at different starch concentrations Table 1. Comparative analysis of amylase activity of C.tropicalis ASY2, using different starchy wastes (adopted from Chaturvedi et al, [19])…”

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“…, respectively at 120 h of fermentation compared to other agro-starchy wastes (Table 1) ) by R. glutinis [19] on 8 th day of fermentation( Table 1). The yeast strain ASY2 produced higher amylase activity of 1.51 IU mL -1 as compared to Rhodotorula glutinis, Lipomyces starkeyi and other agricultural wastes [19]. Based on the statistical analysis, the starch concentration at 20 g L -1…”

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Amylolytic Potential of Oleaginous Yeast in Sago Processing Wastewater (SWW) under Submerged Fermentation

Thangavelu¹,

Sundararaju²,

Srinivasan

et al. 2019

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Sago processing wastewater was assessed for their suitability as growth substrates using oleaginous yeasts, for the production of a useful enzyme (amylase) under submerged fermentation (SmF). Sago wastewater (pH was adjusted to 6) containing starch concentration (10% w/v) were inoculated with yeast strain and incubated at 30ºC for 10 d in an incubator shaker (150 rpm). The results of the amylase activity of oleaginous yeast and in its substratum SWW were compared with the different processing wastes (potato peel, banana peel, cassava peel, corn residue, rice husk, wheat bran, yam peel and barley husk) and oleaginous yeasts (Rhodotorula mucilaginosa, Saccharomyces pastorianus, Lipomyces starkeyi and Rhodotorula glutinis). Compared to other oleaginous yeast, our yeast strain found to produce higher amylase activity of 1.51 IU mL-1. Furthermore, SWW produced more amylase activity than the other compared wastes. This research finding illustrates the environmental friendly and alternate use of sago processing wastewater, towards their valorization as substrates for valuable enzymes and chemical production.

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Valorization of agro-starchy wastes as substrates for oleaginous microbes

Cited by 34 publications

References 52 publications

Overexpression and repression of key rate‐limiting enzymes (acetyl CoA carboxylase and HMG reductase) to enhance fatty acid production from Rhodotorula mucilaginosa

Overexpression and repression of key rate‐limiting enzymes (acetyl CoA carboxylase and HMG reductase) to enhance fatty acid production from Rhodotorula mucilaginosa

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

Amylolytic Potential of Oleaginous Yeast in Sago Processing Wastewater (SWW) under Submerged Fermentation

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