Validity of Estimation of Haemoglobin Content in Dried Blood Spot Samples

Gupta, Aakriti; Kapil, Umesh; Khenduja, Preetika; Yadav, Chander Prakash; Sofi, Nighat Yaseen; Khandelwal, Ritika

doi:10.1007/s12288-016-0766-8

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…Similar to filter paper C in our study, a study was done to validate the DBS taken directly on filter paper and concluded that the filter paper method is an effective and convenient method for conducting surveys, but only after applying a correction factor. [ 8 ]…”

Section: Discussionmentioning

confidence: 99%

“…This DBS of 5 mm in diameter was transferred in a labelled test tube containing 1.25 ml Drabkin solution (DBS of 5 mm in diameter is considered to contain 5 μl of blood). [ 8 ]…”

Section: Methodsmentioning

confidence: 99%

“…[ 5 ] However, studies using indirect methods reported overestimation or underestimation of anaemia prevalence. [ 6 7 8 ] Although, this indirect method is easy to implement and helpful in those situations where laboratory setup is absent or located far from the point of sample collection, skill and training of staff for following strict methodology is required. The standard operating procedures (SOPs) for finger prick and use of micropipette for sample collection and suspension are areas where maximum accuracy needs to be applied.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of indirect cyanmethaemoglobin method and TrueHb haemometer point of care test with direct cyanmethaemoglobin (gold standard) method for strengthening anaemia detection at primary health care level

Khurana,

Aggarwal,

Thungapathra

et al. 2024

Journal of Family Medicine and Primary Care

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Introduction: The most reliable indicator for anaemia diagnosis at the population level is haemoglobin (Hb) estimation. The direct cyanmethaemoglobin method is considered the gold standard method for haemoglobin estimation. However, for resource constraint areas like primary health care (PHC) level, either blood samples are transported on filter paper for Hb testing (indirect cyanmethaemoglobin method) in laboratory or point of care testing is commonly used. Therefore, a comparative analysis of haemoglobin estimation of direct with indirect cyanmethaemoglobin method and also with TrueHb (Wrig Nanosystems Pvt. Ltd.) haemometer was done to strengthen anaemia diagnosis at the PHC level. Materials and Methods: This was a cross-sectional study. A total of 90 participants above 9 years of age, who visited the outpatient department (OPD) of health centre, Kheri and gave consent were included. Comparative analysis was done between Hb concentration assessed by indirect cyanmethaemoglobin method and TrueHb haemometer device against the gold standard method. Results: The mean Hb value estimated by direct method, TrueHb haemometer and indirect methods (filter paper A, B and C) was 11.42 ± 1.59 g/dl, 11.52 ± 1.54 g/dl, 10.66 ± 1.52 g/dl, 9.84 ± 1.50 g/dl and 10.19 ± 1.62 g/dl, respectively. There was no significant difference found between the mean Hb concentration estimated by the direct method and the TrueHb haemometer device. However, there was a significant difference in mean Hb values between the direct method and the indirect method. Therefore, regression analysis was done to estimate the correction factor for the indirect method. Conclusion: TrueHb metre device gave promising results in comparison to the gold standard method and can be used if resource permits in PHC centres. Indirect methods of haemoglobin estimation can be an alternative in resource-constraint settings, specifically for surveys. However, further studies are required for the validation of the indirect method.

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Section: Discussionmentioning

confidence: 99%

“…This DBS of 5 mm in diameter was transferred in a labelled test tube containing 1.25 ml Drabkin solution (DBS of 5 mm in diameter is considered to contain 5 μl of blood). [ 8 ]…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of indirect cyanmethaemoglobin method and TrueHb haemometer point of care test with direct cyanmethaemoglobin (gold standard) method for strengthening anaemia detection at primary health care level

Khurana,

Aggarwal,

Thungapathra

et al. 2024

Journal of Family Medicine and Primary Care

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Comparative analysis of three methods from dried blood spots for expeditious DNA extraction from mosquitoes; suitable for PCR based techniques

2018

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Reproducibility and Validity of Capillary Dried Blood Spot for Diagnosis of Vitamin A Deficiency and Anemia in Children <5 Years Old

Pereira

Silva

Bezerra

et al. 2021

The Journal of Nutrition

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Background Vitamin A deficiency (VAD) and anemia are the most prevalent nutritional deficiency in children globally. The dried blood spot (DBS) method has been used in prevalence studies of VAD and anemia in different age groups. However, it has not yet been validated for children. Objectives This study aimed to assess the reproducibility and validity of DBS in the diagnosis of VAD and anemia in preschoolers. Methods Venous and capillary blood samples were collected from a representative sample of children <5 y old who attended the public health system in Rio de Janeiro. Serum retinol and hemoglobin were measured in 235 and 182 children, respectively. Serum retinol was measured with HPLC and hemoglobin was measured with spectrophotometry in samples of venous (gold standard) and capillary blood (test method, DBS). DBS reproducibility was assessed with the intraclass correlation coefficient (ICC), κ, and prevalence-adjusted and bias-adjusted κ (PABAK). DBS validity was assessed with sensitivity, specificity, accuracy index (AI), positive predictive value (PPV), and negative predictive value (NPV). Results DBS showed very good reproducibility for serum retinol (ICC = 0.94, κ = 0.83, PABAK = 0.76) and very good/good reproducibility for hemoglobin (ICC = 0.86, κ = 0.69, PABAK = 0.69). Prevalence rates for VAD by the reference and test methods were 11.5% and 11.9%, respectively, whereas the anemia rates were 19.2% and 46.2%. The test method showed low sensitivity (33%) and PPV (32%) and high specificity (91%) and NPV (92%) for serum retinol. For hemoglobin, the test method showed fair sensitivity (71%), low PPV (30%), fair specificity (60%), and high NPV (90%). AI was 83% for VAD and 62% for anemia. Conclusions The results suggest that DBS is adequate for the diagnosis of VAD in preschool children, but not for anemia.

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Validity of Estimation of Haemoglobin Content in Dried Blood Spot Samples

Cited by 4 publications

References 2 publications

Comparison of indirect cyanmethaemoglobin method and TrueHb haemometer point of care test with direct cyanmethaemoglobin (gold standard) method for strengthening anaemia detection at primary health care level

Comparison of indirect cyanmethaemoglobin method and TrueHb haemometer point of care test with direct cyanmethaemoglobin (gold standard) method for strengthening anaemia detection at primary health care level

Comparative analysis of three methods from dried blood spots for expeditious DNA extraction from mosquitoes; suitable for PCR based techniques

Reproducibility and Validity of Capillary Dried Blood Spot for Diagnosis of Vitamin A Deficiency and Anemia in Children <5 Years Old

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