Validity of a Portable Glucose, Total Cholesterol, and Triglycerides Multi-Analyzer in Adults

Abstract

This study investigated the accuracy and precision of the Accutrend Plus system to determine blood glucose, total cholesterol, and plasma triglycerides in adults and evaluated its efficiency in measuring these blood variables. The sample consisted of 53 subjects (≥ 18 years). For blood variable laboratory determination, venous blood samples were collected and processed in a Labmax 240 analyzer. To measure blood variables with the Accutrend Plus system, samples of capillary blood were collected. In the analysis, the following tests were included: Wilcoxon and Student's t-tests for paired samples, Lin's concordance coefficient, Bland-Altman method, receiver operating characteristic curve, McNemar test, and k statistics. The results show that the Accutrend Plus system provided significantly higher values (p ≤ .05) of glucose and triglycerides but not of total cholesterol (p > .05) as compared to the values determined in the laboratory. However, the system showed good reproducibility (Lin's coefficient: glucose = .958, triglycerides = .992, total cholesterol = .940) and high concordance with the laboratory method (Lin's coefficient: glucose = .952, triglycerides = .990, total cholesterol = .944) and high sensitivity (glucose = 80.0%, triglycerides = 90.5%, total cholesterol = 84.4%) and specificity (glucose = 100.0%, triglycerides = 96.9%, total cholesterol = 95.2%) in the discrimination of high values of the three blood variables analyzed. It could be concluded that despite the tendency to overestimate glucose and triglyceride levels, a portable multi-analyzer is a valid alternative for the monitoring of metabolic disorders and cardiovascular risk factors.

Reliability and criterion validity of self-measured waist, hip, and neck circumferences

“…Although no reports of error as a percent of body circumferences could be located and a clinically meaningful difference for inter-observer or intra-observer measurements of waist circumference [ 46 ], or other body circumferences, could not be gleaned from the literature, Verweij et al [ 46 ] proposed that a 5 % change in waist circumference measurements taken by trained health professionals may be a clinically relevant short-term change for improvements in health conditions positively associated with waist circumference (e.g., cardiovascular disease). The > ±10 % level was identified as the clinically significant level for this study after considering the inter-observer differences in measurements among trained health professionals reported by others [ 46 , 58 ], Verweij et al’s [ 46 ] “realistic” range of waist circumferences (23.6 inches [60 cm] to 53.15 inches [135 cm]), the current lack of guidance with regard to body circumferences, and examination of studies comparing tests for other measures (i.e., blood glucose, vitamin D, total cholesterol, and triglycerides) which deemed values exceeding approximately 7 to 15 % as clinically significant measurement errors [ 59 – 62 ].…”

Prevalence and factors associated with hypertriglyceridemic waist in the elderly: a population-based study

“…The cutoff points used to define abdominal obesity were > 88 cm for women and > 102 cm for men 13 . To measure 12 hours fasting triglycerides, the Accutrend ® Plus system was used (Roche Diagnostics, Germany), as previously validated 14 . Capillary blood samples were collected through transcu-taneous puncture on the medial side of the tip of the middle finger using a disposable hypodermic lancet.…”

Associations of β-hydroxybutyrate, cholesterol, triglycerides and high-density lipoproteins to non-esterified fatty acids pre- and postpartum

“…However, we found that an equal combination of BHBA, cholesterol and TAG generated better results and thus the use of hand-held devices for measuring cholesterol and TAG in cows should be considered. Hand-held devices for cholesterol and TAG are already validated for the use in human medicine (Coqueiro et al 2013; Ferreira et al 2015) and have been used in cats to measure blood TAG concentrations (Kluger et al 2010). For our purpose, however, the measuring ranges in human medicine are too high (Kluger et al 2010) and have to be adapted to cows.…”

“…Hand-held devices for measuring cholesterol, triglycerides (TAG), and high-density lipoproteins (HDL) are commercially available in human medicine (Accutrend ® Plus system, Roche Diagnostics, Indianapolis, USA; CardioChek ® PA, PTS Diagnostics, Indianapolis, USA). These devices have been shown to be a valid alternative to laboratory analysis (Coqueiro et al 2013; Ferreira et al 2015).…”