Validation of two enzyme immunoassays for aminoglycoside residues according to European Decision 657/2002

Diana, Francesca; Paleologo, Maurizio; Persic, Lidija

doi:10.1080/02652030701458097

Cited by 5 publications

(2 citation statements)

References 11 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Precision under repeatability conditions refers to a precision estimate obtained from replicate measurements made in one laboratory by a single analyst using the same equipment over a short time scale. For each matrix, recovery and precision under repeatability conditions were studied, spiking 18 blank samples at 0.5, 1 and 2 times the MRL (six replicates per concentration level) (Diana et al 2007). Recovery can be defined as the fraction of the analyte determined after addition of a known amount of the analyte to a sample.…”

Section: Precision and Recoverymentioning

confidence: 99%

Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

Solomun

Bilandžić

Varenina

et al. 2010

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) was used for the qualitative screening analysis of neomycin in food of animal origin (muscle, liver, kidney, eggs and milk) at levels corresponding to the European Union maximum residue limit (MRL) set for this substance. The method validation was performed according to the criteria of Commission Decision 2002/657/EC established for qualitative screening methods. In this regard, the following parameters were determined: detection capability (CCβ), specificity, detection limit (LOD), quantification limit (LOQ), recovery, precision, linearity and ruggedness. LODs ranged from 5.7 microg kg(-1) in kidney to 29.3 microg kg(-1) in milk; LOQs ranged from 11.4 microg kg(-1) in kidney to 59.7 microkg(-1) in eggs. The recoveries from spiked samples at the MRL, half the MRL and double the MRL levels ranged from 65.8% to 122.8%, with a coefficient of variation (CV) between 5.9% and 28.6%. The CCβ value was less than the MRL for all examined matrices. Moderate variations of some critical factors in the sample pretreatment for muscle, milk and eggs were deliberately introduced for ruggedness evaluation and had a slight but not statistically significant effect on method performance. The proposed method is suitable for qualitative screening analysis of neomycin in the above-mentioned food in conformity with current European Union performance requirements.

show abstract

Section: Precision and Recoverymentioning

confidence: 99%

Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

Solomun

Bilandžić

Varenina

et al. 2010

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

show abstract

“…The enzyme linked immunosorbent assays (ELISA) and radioimmunoassay have been commonly used for the determination of aminoglycosides in kidney, milk and some biological samples. [8][9][10] However, the aforementioned methods could not provide satisfactory results for the multi-residue quantitation of aminoglycosides. Chromatography is an efficient separation method.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of aminoglycoside antibiotics in feeds using high performance liquid chromatography with evaporative light scattering detection

Liu

Song

et al. 2017

RSC Adv.

View full text Add to dashboard Cite

show abstract

Development of enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for the rapid detection of gentamicin in chicken muscle and milk

Cheng²,

Fan³

et al. 2022

Chinese Journal of Analytical Chemistry

View full text Add to dashboard Cite

Validation of two enzyme immunoassays for aminoglycoside residues according to European Decision 657/2002

Cited by 5 publications

References 11 publications

Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

Simultaneous determination of aminoglycoside antibiotics in feeds using high performance liquid chromatography with evaporative light scattering detection

Development of enzyme-linked immunosorbent assay and colloidal gold-based immunochromatographic assay for the rapid detection of gentamicin in chicken muscle and milk

Contact Info

Product

Resources

About