Validation of Three Different Sterilization Methods of Tilapia Skin Dressing: Impact on Microbiological Enumeration and Collagen Content

Abstract

Tilapia fish skin has demonstrated promise as a stable and practical biological dressing to be used in wound and burn management. However, the appropriate sterilization technique of the Tilapia fish skin is crucial before its clinical application. The standard sterilization technique must eliminate harmful pathogens but maintain the structural and biochemical properties that could compromise the dressing function. This study investigated and compared the efficiency of three sterilizing agents; chlorhexidine gluconate 4% (CHG), povidone iodine 10% (PVP-I), and silver nanoparticles (25 μg/mL) (AgNPs), at three different times (5, 10, and 15 min) on Tilapia fish skin based on the microbial count, histological and collagen properties. Among the sterilization procedures, AgNPs showed rapid and complete antimicrobial activity, with a 100% reduction in microbial growth of the fish skin throughout the treated times. Furthermore, AgNPs did not impair the cellular structure or collagen fibers content of the fish skin. However, CHG and PVP-I caused alterations in the collagen content. This study demonstrated that the AgNPs treatment of Tilapia fish skin provided sterile skin while preserving the histological properties and structural integrity. These findings provide an efficient and quick sterilization method suitable for Tilapia fish skin that could be adopted as a biological dressing.

Recent progress in nanocomposites of carbon dioxide fixation derived reproducible biomedical polymers

“…Collagen, the general name of a series of proteins with molecular weight (Mw) ~300,000, is the most abundant protein in animal body and the major component of skin and skeletal muscle tissues (Chen et al, 2008;Arias et al, 2018;Le Corre-Bordes et al, 2018;Bi et al, 2019;Dippold et al, 2019;Ibrahim et al, 2020;García-Hernández et al, 2021;Hernández-Rangel and Martin-Martinez, 2021). To date, over 20 different types of collagen have been detected in human body, the most common being collagen types I-IV.…”

Polyethylene Oxide Assisted Fish Collagen-Poly-ε-Caprolactone Nanofiber Membranes by Electrospinning

“…Therefore, the extraction of collagen from Tilapia skin (FC) and further development and utilization can not only increase the added value of products, but also avoid resource waste and reduce environmental pollution [ 2 ]. It has also been reported that the Tilapia skin collagen film-forming property is significantly better than that of freshwater fish such as carp ( Hypophthalmichthys molitrix ) and grass carp ( Ctenopharyngodon idella ) [ 3 ].…”

The optimal concentration of silver nanoparticles in sterilizing fish skin grafts

“…Tilapia skin is also a highly available product, safe, innovative, and easy to apply materials. Thus, it could be suggested as a potential xenograft for treating burns and regenerative medicine 3 .…”

“…However, collagen integrity should be considered in the sterilization of fish skin grafts along with the efficient antimicrobial effect of the sterilizing agent. In a previous study, Ibrahim et al 3 , investigated that the silver nanoparticles (Ag NPs) explored advantage over both; chlorhexidine gluconate 4% and povidone iodine 10% in sterilizing of fish skin based on the microbial count and the collagen fiber integrity. However, this study had a limited use of one concentration of Ag NPs (25 µg/mL).…”

“…The optimal concentration of silver nanoparticles in sterilizing fish skin grafts Abdelnaby M. Elshahawy 1* , Ghada Abd-Elmonsef Mahmoud 2* , Doaa M. Mokhtar 3,4 & Ahmed Ibrahim 5* Collagen integrity should be considered on using a sterilizing agent for fish skin grafts. This study defined the optimal concentration of silver nanoparticles (Ag NPs) for sterilization of fish skin grafts without disrupting collagen content based on microbiological and histological evaluation.…”