Polyethylene Oxide Assisted Fish Collagen-Poly-ε-Caprolactone Nanofiber Membranes by Electrospinning

Abstract

Fish collagen has higher biocompatibility and lower immunogenicity than terrestrial collagen, and is currently one of the important raw materials for preparing biological materials. In this study, PEO was used as a spinning aid to prepare fish skin collagen-PCL nanofiber membranes by electrospinning, and the process was optimized to get smooth nanofibers. The morphological and mechanical properties of collagen-PCL nanofiber membranes were assessed by scanning electron microscopy (SEM). The changes in chemical composition due to the incorporation of collagen into PCL and PEO were determined by Fourier Transform infrared spectroscopy (FTIR). The biocompatibility of the collagen-PCL nanofiber membranes was evaluated in vitro in cultures of mouse fibroblasts and in vivo by subcutaneous implantation studies in rats. It was found that the diameter of the spun fibers became fine and smooth when the ratio of the collagen/PCL increased. The finally obtained nanofiber had good mechanical strength, porosity, and hydrophilicity, and could promote cell adhesion and proliferation. The FC-PCL nanofiber membrane prepared by this route opens a new way to prepare fish collagen biomaterials with electrospinning.

Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds

“…In this case, the diameter of the fibers became fine and smooth as the ratio of the collagen/PCL increased. The obtained nanofibers had good mechanical strength, porosity, and hydrophilicity, and could promote cell adhesion and proliferation [71].…”

Preparation of PVA/PEI/CNC/ZnO composite membrane with good mechanical properties and water resistance by electrostatic spinning using for efficient filtration of PM2.5

“…Though high filtration efficiency could be achieved by increasing its thickness, this would cause high‐pressure drop 11 . While electrospun nanofibrous membrane with diameters ranging from micrometer to nanometer level, have the advantages of high specific surface area, controllable structure, and excellent internal connectivity, being able to filter fine particle under the combined depressurization 12 . For example, the nanofibrous membrane made from polyurethane and polyvinylidene fluoride polymers by electrostatic spinning had strong hydrophobicity, high tensile strength, and excellent air filtration efficiency.…”

“…11 While electrospun nanofibrous membrane with diameters ranging from micrometer to nanometer level, have the advantages of high specific surface area, controllable structure, and excellent internal connectivity, being able to filter fine particle under the combined depressurization. 12 For example, the nanofibrous membrane made from polyurethane and polyvinylidene fluoride polymers by electrostatic spinning had strong hydrophobicity, high tensile strength, and excellent air filtration efficiency. Polyacrylonitrile was also utilized to prepare three types of composite nanofibrous membranes with disordered, standard-ordered, and cross ordered for air filtration.…”

Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

“…Shue et al fabricated a series of composite fibrous membranes incorporated with fish collagen, nanohydroxyapatite, and poly (lactic-co-glycolic acid) (PLGA) to guide bone regeneration [ 14 ]. He et al prepared collagen-polycaprolactone (PCL) nanofiber membranes, which had diameters of at least 150 nm [ 15 ]. However, marine composite fibrous membranes often have limited mechanical properties and poor thermal stability, which hampers their use in tissue repair [ 16 ].…”