Validation of the use of Pap‐stained vaginal smears for diagnosis of bacterial vaginosis

Eriksson, Katarina; Forsum, Urban; Bjørnerem, Åshild; Platz-Christensen, JJ; Larsson, Per‐Göran

doi:10.1111/j.1600-0463.2007.apm_607.x

Cited by 21 publications

(27 citation statements)

References 28 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5 The sensitivity of Pap tests for diagnosing BV varies from 90% to as low as 43%; the wide range of results reflects the use of different morphologic criteria and whether the specimens were obtained from the cervix or the vagina. 5,[21][22][23][24][25] Similarly, the Pap test lacks sensitivity as a screening test for Trichomonas. Moreover, in low-prevalence populations, Trichomonas found by cytomorphologic review and reported by routine Pap testing frequently represented a false-positive result.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis

Levi

Harigopal

Hui

et al. 2011

American Journal of Clinical Pathology

View full text Add to dashboard Cite

To compare the Affirm VPIII molecular test (Becton Dickinson, Burlington, NC) with morphologic identification used in routine Papanicolaou (Pap) test screening in the detection and identification of Candida species, Trichomonas vaginalis, and Gardnerella vaginalis, we identified 431 cases with a concomitant Pap test and Affirm VPIII assay performed from the archives of a large academic institution. The study population consisted of women ranging in age from 17 to 79 years (mean and median ages, 33 and 31 years, respectively). With a routine Pap test, 60 patients (13.9%) were found to have bacterial vaginosis, 60 (13.9%) candidiasis, and 3 (0.7%) Trichomonas infection. With the Affirm VPIII assay, 183 (42.5%) patients tested positive for G vaginalis, 70 (16.2%) positive for Candida species, and 10 (2.3%) positive for T vaginalis. The differences were statistically significant. The results demonstrate that our patient population had a high incidence of bacterial vaginosis/Candida vaginitis; however, the Affirm VPIII was a more sensitive diagnostic test for the detection and identification of all 3 organisms compared with the Pap test.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis

Levi

Harigopal

Hui

et al. 2011

American Journal of Clinical Pathology

View full text Add to dashboard Cite

show abstract

“…El sistema de puntuación de Nugent es una prueba diagnóstica para la VB que ha sido validada y utilizada como prueba de oro en otras poblaciones (10,14,(23)(24)(25)(26)(27)(28). La decisión de implementar este sistema diagnóstico como prueba de rutina en las mujeres embarazadas de nuestro país debe ser sustentada en evidencia científica sobre su utilidad en nuestra población.…”

Section: Discussionunclassified

“…En este estudio utilizamos el análisis ROC para determinar el mejor punto de corte del sistema de puntuación de Nugent el cual correspondió a un valor de 5 o más qué es diferente al punto de corte tradicionalmente usado en otros estudios (10,14,(23)(24)(25)(26)(27)(28). Sin embargo, la sensibilidad, especificidad y valores predictivos encontrados en este estudio fueron superiores a los reportados previamente en esos estudios.…”

Section: Discussionunclassified

Validez Y Reproducibilidad Del Sistema De Puntuación De Nugent Para El Diagnóstico De Vaginosis Bacteriana en Mujeres Embarazadas

Arámbula

2009

Rev. chil. obstet. ginecol.

View full text Add to dashboard Cite

a Médico Cirujano, magíster en Epidemiología.b Ingeniera de Sistemas, magíster en Bioestadística. c Bacterióloga, magíster en Microbiología.* Investigación financiada por la Universidad Industrial de Santander. RESUMENAntecedentes: La vaginosis bacteriana se encuentra entre 15 a 20% de las mujeres embarazadas y el método rutinario para diagnosticarla es el estudio del flujo vaginal aplicando los criterios de Amsel. Sin embargo, existen otros métodos diagnósticos como el sistema de Nugent, que ha mostrado adecuada validez a menor costo. Objetivo: Evaluar la validez y reproducibilidad del sistema de Nugent para el diagnóstico de vaginosis bacteriana en embarazadas. Método: Estudio de evaluación de pruebas diagnósticas. Se estudiaron 100 mujeres con embarazo de bajo riesgo. A cada paciente se le tomaron dos muestras de secreción de flujo vaginal y tres evaluadores examinaron cada muestra. Se evaluó la validez de los criterios de Nugent comparándolos con los de Amsel (prueba de oro) y se obtuvo el mejor punto de corte usando la curva del receptor-operador (ROC), para calcular la sensibilidad, especificidad y valores predictivos. La reproducibilidad se midió con el coeficiente Kappa. Resultados: El promedio de edad fue 25,6 años y edad gestacional 23 semanas. Un 19% había tenido infección vaginal previamente. La prevalencia actual de vaginosis bacteriana fue 16% (IC95%: 9,43%; 24,67%). El área bajo la curva ROC fue 0,98 (IC95%: 0,95; 1,00). La sensibilidad fue 1,00 (IC95%: 0,77; 1,00), especificidad 0,96 (IC95%: 0,90; 0,99), valor predictivo positivo 0,82 (IC95%: 0,57; 0,96) y valor predictivo negativo 1,00 (IC95%: 0,95; 1,00). Conclusión: El sistema de Nugent para el diagnóstico de vaginosis bacteriana en embarazadas es una prueba válida y reproducible para implementar rutinariamente durante el control prenatal.PALABRAS CLAVE: Vaginosis bacteriana, validez diagnóstica, reproducibilidad, prueba de Nugent SUMMARY Background:The bacterial vaginosis is found in 15% to 20% of the pregnant women and the study of the vaginal discharge using the Amsel criteria is the usual method to diagnose it. However, there are other diagnostic methods like the Nugent Score System which has shown to be accurate and less expensive. Objective: To evaluate the accuracy and reliability of the Nugent Score System for diagnosing bacterial vaginosis in pregnant women. Methods: This is a study for evaluating medical tests. We studied 100 women who had a low risk pregnancy. Two samples of the vaginal discharge were taken in each woman and three masked

show abstract

“…Cytological diagnosis, through Pap smears, is a widely accepted screening method for the detection of precancerous lesions and early cervical cancer. Because it is a fast and inexpensive examination, several studies have demonstrated the importance of the Papanicolaou method for the diagnosis of BV, with good sensitivity, specificity and positive predictive value …”

Section: Introductionmentioning

confidence: 99%

Protease inhibitor effects on prevalence of bacterial vaginosis and human papillomavirus‐related lesions

Marques

Pinto

Soares

et al. 2020

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim: Antiretrovirals of the protease inhibitor (PI) class tend to achieve low concentrations in biological fluids. This study aimed to analyze possible changes in the vaginal microbiome and frequency of cervical human papillomavirus (HPV)-DNA and HPV-related lesions associated with the use of PI in antiretroviral therapy (ART). Methods: Eighty-eight women with human immunodeficiency virus infection were divided in two groups: ART with PI and without PI. All the participants underwent anamnesis with demographic data collection. The total DNA, used as the template in the polymerase chain reaction-based assays for the detection of HPV-DNA, was extracted from cervical samples during cervical cytopathology.Results: There were no differences between the groups with respect to HPV-related lesions. Despite the higher prevalence of bacterial vaginosis (BV) in the PI group (33.96% vs 17.14%), the difference was insignificant when considering all women (P = 0.066). When women with a detectable viral load and a CD4+ T-cell count <200 were excluded in both groups, BV was found to be more prevalent in the PI group (odds ratio, 3.349; 95% confidence interval, 1.113-11.41, P = 0.049). No associations were found between BV and age, condom use, cervical HPV, time with current ART regimen, unprotected receptive anal intercourse and cervical HPV-related lesions. Conclusion: The use of PI did not alter the frequencies of HPV-DNA and HPV-related lesions. However, an increased frequency of BV was found in women using PI after excluding women with a detectable viral load and a CD4+ T-cell count of <200. Key words: benign disease of vulva and vagina, cytology and gynecologic pathology, human papillomavirus infection and cervical intraepithelial neoplasia.

show abstract

Validation of the use of Pap‐stained vaginal smears for diagnosis of bacterial vaginosis

Cited by 21 publications

References 28 publications

Comparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis

Comparison of Affirm VPIII and Papanicolaou Tests in the Detection of Infectious Vaginitis

Validez Y Reproducibilidad Del Sistema De Puntuación De Nugent Para El Diagnóstico De Vaginosis Bacteriana en Mujeres Embarazadas

Protease inhibitor effects on prevalence of bacterial vaginosis and human papillomavirus‐related lesions

Contact Info

Product

Resources

About