Validation of the Species Concept in the Genus Dekkera by Restriction Analysis of Genes Coding for rRNA

Abstract

The gene coding for the small-subunit rRNA of 11 type strains belonging to the genus Dekkera and its anamorph, Brettanomyces, was amplified by using the polymerase chain reaction and subjected to digestion with a series of restriction endonucleases. Similarity coefficients were calculated from the number of shared and unique fragments, and a cluster analysis yielded four distinct groups with the following ascosporogenous states: Dekkera anomah, Dekkera bruxellensis, Dekkera custersiana, and Dekkera naardenensis. Results correlate with evidence from isoenzyme electrophoresis and DNA homology analysis. They also confirm previously reported anamorph-teleomorph connections and recently proposed synonymies within the genus.Most classification schemes for the genus Dekkera, the ascosporogenous genus established by van der Walt in 1964 (20) to accommodate the sexual state of the genus Brettanoinyces, are based on morphological and physiological characteristics (2,20,21). In their recent account of the genus, Barnett and coworkers (2) recognized four species: Dekkera iinomala (syn. Dekkera claussenii), Dekkera bruxellensis (syn. Dekkera abstinens, Dekkera intermedia, and Dekkera lambica), Dekkera custersiana , and Dekkera naardenensis .

The Phylogenetic Relationships of Species of the GenusDekkeravan derWaltBased on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)

“…Subsequently, Molina et al 8 ) reported a similar phenogram based on the results obtained from the restriction analyses of genes coding a small-subunit (18S) rRNA. Our experimental data described above were very similar to the phylogenetic tree reported by Clark-Walker et al 7 ) and to the phenogram reported by Molina et al, 8) especially in the 18S rRNA partial base sequencings of Dekkera and Brettanomyces species. In the 26S rRNA partial base sequencings, D. naardenensis was, however, found to be phylogenetically distant (percent similarities, 48-56; base differences 46-38).…”

“…2 6) The species classified presently in the teleomorphic genus Dekkera (and anamorphic genus Brettanomyces) are restricted to four: 4 ,5) The molecular-phylogenetic studies of the species in the genera Dekkera and Brettanomyces were first done by Clark-Walker et al 7 ) on the analyses of the genomic maps of their mitochondrial DNA, and followed by Molina et al 8) on the restriction analyses of their genes coding for the small subunit of rRNAs.…”

Phylogeny of the Yeast Genera Hanseniaspora (Anamorph Kloeckera), Dekkera (Anamorph Brettanomyces), and Eeniella as Inferred from Partial 26S Ribosomal DNA Nucleotide Sequences

“…Species have been defined on the basis of nuclear DNA relatedness data and the results of electrophoretic comparisons of cellular enzymes (25) and by ribotyping (15). Currently, four Brettanomyces species are distinguished: these species are Brettanomyces anomalus, Brettanomyces bruxellensis, Brettanomyces custersianus, and Brettanomyces naardenensis.…”

The significance of its-RFLPs and coenzyme Q system in determining taxonomic relationships among Candida species.

“…Nuclear internal transcribed spacers (ITS) have provided an efficient means for the investigation of genetic relationship in yeasts (8,12). Lott et al (9) analyzed the nucleotide sequences of the nuclear 5.8S and ITS2 regions from multiple isolates of five Candida species.…”