Validation of the multiplex ligation‐dependent probe amplification assay and its application on the distribution study of the major alleles of 17 blood group systems in <scp>C</scp>hinese donors from <scp>G</scp>uangzhou

Ji, Yanli; Wen, Jinxiu; Veldhuisen, Barbera; Haer‐Wigman, Lonneke; Wang, Zhen; Straaten, Martin Lodén-van; Wei, Ling; Luo, Guopei; Fu, Yongshui; Schoot, C. Ellen van der

doi:10.1111/trf.13940

Cited by 8 publications

(25 citation statements)

References 40 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are six different MNS phenotypes in the 97 Mi a ‐positive Asian American donors (Table ), which are the same profile identified in the 200 Chinese donors in Guangzhou . Among the six MNS phenotypes, the frequencies of M+ N‐ S‐ s+ and M+ N+ S‐ s+ phenotypes are 48.5% and 38.1% in this study compared to 26.1% and 45.2% in the 200 donors in Guangzhou, respectively .…”

Section: Discussionsupporting

confidence: 73%

“…Using a combination of serologic typing and the multiplex ligation‐dependent probe amplification analysis, Wei and coworkers identified 48 GYP*Mur heterozygotes, two GYP*Mur homozygotes, and one GYP*Bun heterozygote from 528 Chinese donors in Guangzhou . In addition, Ji and coworkers screened 200 blood donors from southern China and identified 18 individuals with the GYP*Mur allele . Using the restriction fragment length polymorphism analysis, Shih and coworkers screened 264 Taiwanese blood donors and reported 13 individuals with the GYP*Mur allele .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

et al. 2019

View full text Add to dashboard Cite

BACKGROUND The GP.Mur glycophorin with Mia phenotype is relatively common and clinically significant in the Southeast Asian populations. The aim of this study is to genotype Mia‐positive Asian American type O blood donors. Red blood cell (RBC) minor antigens were also determined in the same cohort. STUDY DESIGN AND METHODS Asian American blood donors of the Gulf Coast Regional Blood Center (Houston, TX) were screened using a typing reagent (NOVACLONE Anti‐Mia Monoclonal IgG Typing Reagent, Dominion Biologicals Ltd) from March 2016 to July 2018. Aliquots of Mia‐positive blood from type O donors were subjected to serologic confirmation using Mia‐ and/or Mur‐specific GAMA210 and 64D6 monoclonal antibodies, and two human antisera. Extracted genomic DNA was amplified by polymerase chain reaction (PCR) using GYP hybrid gene/allele‐specific primers followed by bidirectional Sanger sequencing. Zygosity for GYP*Mur and GYP*Bun was determined using TaqMan real‐time PCR assay. Phenotypes of 35 RBC antigens and three phenotypic variants were determined with use of an in vitro diagnostic test, PreciseType HEA Molecular BeadChip Test (Immucor). RESULTS By screening 4600 blood donations in the Houston metropolitan area, 209 samples from 103 unique donors were identified to be Mia‐positive. By PCR and sequencing analysis, 97 of the 103 Mia‐positive donors carried hybrid genes GYP*Mur (89.7% including two homozygotes), GYP*Bun (6.2%), GYP*Vw (3.1%) and GYP*Hut (1.0%). Concordance between serology and DNA analysis was 98%, 99%, and 100% for the GAMA210, 64D6, and human antisera, respectively. Genotyping of RBC antigens showed that the Mia‐positive donors were predominantly associated M+ N‐ S‐ s+ (48.5%) and M+ N+ S‐ s+ (38.1%) phenotypes. CONCLUSIONS The GP.Mur glycophorin is most prevalent in the Mia‐positive Asian American type O blood donors.

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For the GYP(B‐A) hybrid allele identification, GYPB ‐specific upstream primer located in GYPB Intron 2 and GYPA ‐specific downstream primer located in GYPA Exon 4 were used to amplify the GYP(B‐A) hybrid gene, with an expected 1277‐bp PCR product. The amplification and direct sequencing were conducted using the primers and protocols described previously . Sequencing data were compared with RefSeqGene GYPB (GenBank NG_007483.2), GYP*HF (M81079), GYP*Mur (AF090739), St a type B (M71244), and GYP*Bun (M60710) by using the SeqMan II program of the Lasergene package (DNASTAR Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…The amplification and direct sequencing were conducted using the primers and protocols described previously. 26 Sequencing data were compared with RefSeqGene GYPB (GenBank NG_ 007483.2), GYP*HF (M81079), GYP*Mur (AF090739), St a type B (M71244), and GYP*Bun (M60710) by using the SeqMan II program of the Lasergene package (DNASTAR Inc.). DNA sequence for another GYP*Bun allele (GenBank KR363627), recently reported with a different intronic breakpoint to the reference GYP*Bun (M60710), was also used for comparison.…”

Section: Dna Sequencing Of the Samples Showing A Variant Melting Curvementioning

confidence: 99%

See 1 more Smart Citation

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Identification of a novel DI02(2558T)* allele associated with weakened expression of DI2 antigen

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: The distribution of DI1/DI2 antigens of the Diego blood group system is polymorphic in Mongoloid populations and the corresponding alloantibodies are clinically significant. Here a novel DI variant was found by donor screening, and the effect of the novel and previously reported mutations on expression of DI1/DI2 antigens and Band 3 protein was explored. Study Design and Methods: DNA samples of 1150 Chinese donors were collected. DI*01/DI*02 genotyping was determined by Sanger sequencing. For the carrier of novel allele, the expression of Band 3 and DI1/DI2 antigens on red blood cells (RBCs) was detected by Western blot and flow cytometry, respectively. in vitro expression studies were conducted by transfecting the mutant (including the novel and three reported DI*02(2534T), DI*02 (2358_2359insCAC), and DI*02(2572T) alleles) or wild-type DI*02 constructs into HEK 293T cells, the expression of Band 3 and DI1/DI2 antigens was analyzed. Results: A novel heterozygous mutation (c.2558C>T, p.Thr853Met), which is located near the DI1/DI2 polymorphism site (c.2561T>C), was identified in a donor with DI:-1,2 phenotype. Reduced expression of DI2 antigen was observed on the RBCs, while weakened expression of Band 3 and absence of DI2 antigen were detected in cells transfected with the mutant DI*02(2558T) construct. In addition, absent or decreased expression of Band 3 and DI2 antigen was also detected in cells transfected with three reported mutant constructs. Conclusion: The novel DI*02(2558T) allele and three previously described DI mutations can affect the expression of Band 3 protein and/or DI2 antigen and/or interfere with DI*01/DI*02 genotyping result.

show abstract

Validation of the multiplex ligation‐dependent probe amplification assay and its application on the distribution study of the major alleles of 17 blood group systems in Chinese donors from Guangzhou

Abstract: The blood-MLPA assay could easily identify the common blood-group alleles and correctly predicted phenotype in the Chinese population. The Mur and St antigens were distributed with high frequency in a Southern Chinese Han population.

Cited by 8 publications

References 40 publications

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

Identification of a novel DI02(2558T)* allele associated with weakened expression of DI2 antigen

Contact Info

Product

Resources

About

Validation of the multiplex ligation‐dependent probe amplification assay and its application on the distribution study of the major alleles of 17 blood group systems in Chinese donors from Guangzhou

Abstract: The blood-MLPA assay could easily identify the common blood-group alleles and correctly predicted phenotype in the Chinese population. The Mur and St antigens were distributed with high frequency in a Southern Chinese Han population.

Cited by 8 publications

References 40 publications

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mia phenotype

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mia phenotype

Genotyping analysis of MNS blood group GP(B‐A‐B) hybrid glycophorins in the Chinese Southern Han population using a high‐resolution melting assay

Identification of a novel DI*02(2558T) allele associated with weakened expression of DI2 antigen

Contact Info

Product

Resources

About

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

Hybrid glycophorin and red blood cell antigen genotyping in Asian American type O blood donors with Mi^a phenotype

Identification of a novel DI02(2558T)* allele associated with weakened expression of DI2 antigen