Validation of the method of identification and quantitative determination of bacitracin in the form of bacitracin derivative with dabsyl chloride by thin-layer chromatography and densitometry

Krzek, Jan; Piotrowska, Joanna; Żuromska-Witek, Barbara; Hubicka, Urszula; Łysoń, Ewelina

doi:10.1556/jpc.26.2013.1.10

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…Bacitracin with dabsyl chloride gives a complex and can be detected by densitometric measurements at 460 nm [58]. …”

Section: Visualizing Effects For Different Substances Including Drugsmentioning

confidence: 99%

“…Bacitracin is a mixture of related cyclic polypeptides produced by Bacillus licheniformis and Bacillus subtilis organisms and is used in human medicine as a polypeptide antibiotic. Bacitracin with dabsyl chloride gives a complex and can be detected by densitometric measurements at 460 nm [ 58 ].…”

Section: Visualizing Effects For Different Substances Including Drmentioning

confidence: 99%

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Detection Progress of Selected Drugs in TLC

Pyka

2014

BioMed Research International

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This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of detection, densitometric visualizing index, and linearity range of detected compounds were used for the evaluation of visualizing effects of applied visualizing reagents. It was shown that visualizing effect depends on the chemical structure of the visualizing reagent, the structure of the substance detected, and the chromatographic adsorbent applied. The usefulness of densitometry to direct detection of some drugs was also shown. Quoted papers indicate the detection progress of selected drugs investigated by thin-layer chromatography (TLC).

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“…Bacitracin with dabsyl chloride gives a complex and can be detected by densitometric measurements at 460 nm [58]. …”

Section: Visualizing Effects For Different Substances Including Drugsmentioning

confidence: 99%

Section: Visualizing Effects For Different Substances Including Drmentioning

confidence: 99%

Detection Progress of Selected Drugs in TLC

Pyka

2014

BioMed Research International

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“…The TLC‐densitometric analysis was performed on silica gel TLC plates with a fluorescent indicator. A mobile phase of n ‐butanol–2‐butanone–25% ammonia–water (10:5:2:2, v/v/v/v) was used as an efficient mobile phase (Krzek et al ., ). This method is very sensitive because the limit of detection (LOD) value is estimated as 8.6 mg per band.…”

Section: Thin‐layer Chromatographymentioning

confidence: 99%

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Dołowy

Pyka

2013

Biomedical Chromatography

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Amino acids are very important organic compounds in nature. The biological activity of amino acids depends mainly on their stereoisomeric configuration (d- or l-). Thus, the stereochemical analysis of amino acids and peptides is an important aspect of their characterization. Owing to the increasing role of amino acid configuration in biomedical and pharmaceutical studies, numerous analytical methods have been described in the literature. Among a wide range of analytical techniques available for the steroselective separation of different amino acids, which were obtained from plants or biological samples, chromatographic methods such as thin-layer chromatography, high-performance chromatography and also gas chromatography are very useful. This review presents such systems developed for direct stereoisomeric separation and quantitative determination of amino acid and peptide enantiomers with emphasis on selected literature published during two last decades. Almost all aspects, including sample preparation prior chromatographic analysis, stationary phase, solvent system and detection system, are discussed. New possibilities in chiral amino acid analysis have been opened up by the application of mass spectrometry and infrared detection in thin-layer chromatography and mass spectrometry-selected ion monitoring detection system in gas chromatography. A modern trend in high-performance chromatography is two-dimensional chromatography. These innovations have led to decreased development time and increased amino acid resolution and detectability.

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Applications of thin-layer chromatography in the pharmaceutical industry

Agbaba¹,

Čarapić²

2023

Instrumental Thin-Layer Chromatography

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Validation of the method of identification and quantitative determination of bacitracin in the form of bacitracin derivative with dabsyl chloride by thin-layer chromatography and densitometry

Cited by 4 publications

References 29 publications

Detection Progress of Selected Drugs in TLC

Detection Progress of Selected Drugs in TLC

Application of TLC, HPLC and GC methods to the study of amino acid and peptide enantiomers: a review

Applications of thin-layer chromatography in the pharmaceutical industry

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