Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids

Fleming, Chérina; Brouwer, Reinoud W; Lindemans, Jan; Jonge, Robert de

doi:10.1515/cclm-2011-0927

Cited by 64 publications

(117 citation statements)

References 15 publications

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“…We evaluated this mode and found good agreement with manual microscopy for counting cells in CSF, serous and CAPD samples [126]. The XN-BF mode overestimated the CSF PMN count compared to microscopy.…”

Section: Xn-bf Modementioning

confidence: 75%

“…Other fluids (CAPD, ascites and pleura fluid), investigated in the same study did not show an overestimation of PMNs. Furthermore, the sensitivity (100%) and specificity (97%) on the XN-BF mode were good for predicting abnormality in CSF (WBC > 6 × 10 6 /L) [126]. In summary, Sysmex analyzers have proven to be accurate and sensitive for processing BF samples while offering advantages, such as cost effectiveness and faster turnaround times.…”

Section: Xn-bf Modementioning

confidence: 91%

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Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease

Fleming

Russcher

Lindemans

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Abstract:In many inflammatory diseases, the cellular components in body fluids [cerebrospinal fluid (CSF), serous fluids] are increased, rendering essential diagnostic information. The diagnostic value of the total white blood cell count (WBC) and differential count has been evaluated extensively over the years, and a remarkable amount of knowledge has been gained; yet, there is a great deal of clinical uncertainty whether the diagnosis should be based solely on these variables. In some diseases, such as peritonitis, the total WBC and differential count has high sensitivity; whereas, in differentiating pleural effusions, it lacks the sensitivity required to be clinically useful. Nevertheless, many guidelines consider these tests as cornerstone parameters, and in combination with clinical variables, they can successfully guide clinical decision making in initiating or postponing a treatment course for infection and/or inflammatory diseases while awaiting culture results. Although other methods are available for detecting and differentiating WBCs in body fluids, manual microscopy is still considered the gold standard despite its many limitations. During the last decade, automated analyzers have become a popular method for first line screening. Continued progress in their design has led to major improvements including their speed, improved accuracy and lower variability compared with microscopy. Disadvantages of this method include high imprecision in low ranges (depending on the method) and interfering factors.In a time where automation is at the front line in clinical laboratories, it is essential the results obtained are precise, accurate and reproducible. This review provides an overview of the relevance for cell counting in a variety of diagnostic body fluids, and highlights the current technologies used.

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Section: Xn-bf Modementioning

confidence: 75%

Section: Xn-bf Modementioning

confidence: 91%

Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease

Fleming

Russcher

Lindemans

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

Self Cite

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show abstract

“…[5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] It is noteworthy that the automated analysis of body fluid cellularity is mostly challenged by the presence of macrophages (MACROs), mesothelial cells (MESOs), and other abnormal or pathological cells (especially solid cancer cells) that are typically absent in blood or urine, so that the accuracy and differentiation of cellular elements may be impaired. 6,7,15,22,24 More specifically, some automated cell count analyzers provide a TNC count but not morphological differentiation, [16][17][18][19][20] whereas specific information about the accuracy of cell recognition is unavailable for others. 8,23,24 Even more importantly, validation rules for automated cell counting and a definition of the reflex testing required for accurate result reporting are unavailable in the current scientific literature to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Reflex Testing Rules for Cell Count and Differentiation of Nucleated Elements in Pleural and Ascitic Fluids on Sysmex XE-5000

et al. 2016

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Flow cytometry is widely used in many laboratories for automated nucleated cell counts and their differentiation in body fluids. The implementation of new reflex testing rules on these automated instruments could open new frontiers in laboratory workflow, improving characterization of body fluids and clinical diagnosis and decreasing costs. Ascitic (150) and pleural (33) fluids were collected and assessed by XE-5000 and optical microscopy. Cell counts performed with the methods showed a Pearson's correlation of 0.98 (p < 0.0001), Passing-Bablok regression y = 0.99x + 2.44, and bias of 32.3. In ascitic fluids, the best diagnostic performance was found for polymorphonuclear and neutrophil counts on XE-5000, which exhibited areas under the curve (AUCs) 0.98 (p < 0.0001) and 0.99 (p < 0.0001), respectively. In pleural fluids the best diagnostic performance was found for polymorphonuclear percent parameter, which displayed 0.97 (p < 0.0001). Specific reflex test rules based on these parameters were characterized by 92% diagnostic concordance, 1.00 sensitivity, and 0.84 specificity with optical microscopy. The application of a set of reflex testing rules may improve the diagnostic performance of XE-5000, increasing its reliability for routine automated cell count in body fluids. We acknowledge that further studies should be planned to validate our findings according to clinical data.

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“…Cells can be easily detected by modern hematology analyzers used for common routine analysis of peripheral blood samples. The BC-6800 is equipped with a dedicated platform for body fluid (BF) examination, like other hematology analyzer [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. More recently CSF analysis has been performed even on automated urine flow cytometers [23][24][25][26] and urine microscopy analyzers [27,28].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting

Buoro

Seghezzi

Mecca

et al. 2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids

Abstract: The BF module on the XN-1000 is a suitable tool for fast and accurate quantification of WBC (differential) and RBC counts in CSF and other BFs in a diagnostic setting.

Cited by 64 publications

References 15 publications

Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease

Clinical relevance and contemporary methods for counting blood cells in body fluids suspected of inflammatory disease

Reflex Testing Rules for Cell Count and Differentiation of Nucleated Elements in Pleural and Ascitic Fluids on Sysmex XE-5000

Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting

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