Validation of quantitative backscattered electron imaging for the measurement of mineral density distribution in human bone biopsies

Roschger, Paul; Fratzl, Peter; Eschberger, J; Klaushofer, Klaus

doi:10.1016/s8756-3282(98)00112-4

Cited by 403 publications

(397 citation statements)

References 15 publications

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“…In this study, the bone mineral density was measured by detecting backscattered electrons using an SEM. As shown by some others [5,35], this methods allows to measure the bone mineralization. However, it was not possible to determine if mineralization itself was impaired by the presence of zoledronate.…”

Section: Discussionmentioning

confidence: 80%

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Peter

Pioletti

Laı̈b

et al. 2005

Bone

252

170

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Despite total hip replacement (THR) gives generally satisfactory results, the quality of outcome in young patients is markedly decreased compared to the average THR outcome. For this population, pharmacological treatment with bisphosphonate would be beneficial to decrease the peri-implant osteolysis. However, as this population does not necessarily suffer from osteoporosis, a nonsystemic treatment would be preferable. Zoledronate was then grafted to hydroxyapatite (HA) coating of titanium implants. The implants were inserted in rat condyles with various zoledronate concentrations. A positive concentration-dependent effect was observed on the peri-implant bone density and on different histomorphometric parameters. Importantly for the outcome of the implants, the mechanical fixation was increased by the local presence of zoledronate.The obtained results open the way of an easy transformation of currently existing HA-coated implants by grafting bisphosphonate onto the coating in order to increase their service life in the patients. D 2004 Elsevier Inc. All rights reserved.

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Section: Discussionmentioning

confidence: 80%

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Peter

Pioletti

Laı̈b

et al. 2005

Bone

252

170

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“…The greater trochanters of the rats were sectioned with a smaller diamondimpregnated blade using a water-cooled bone saw (Isomet; Beuler, Lake Bluff, IL). One coronal section from each specimen was used for light histology of the tendon insertion, and the other coronal section was used for quantitative BSE imaging of the calcified fibrocartilage and cortical bone (Skedros et al, 1993a;Bloebaum et al, 1997;Roschger et al, 1998).…”

Section: Materials and Methods Specimen Preparationmentioning

confidence: 99%

“…This provides a mean value for the backscattered signal independent of porosity (black pixels 0 -5 in the BSE image). BSE-image WMGLs were calibrated at 20-min intervals using pure aluminum and carbon as calibration standards Roschger et al, 1998). The use of pure elements provides a simple method of confirming beam stability during the course of an experiment (Grynpas et al, 1994;Roschger et al, 1995;Vajda et al, 1995).…”

Section: Bse Analysismentioning

confidence: 99%

“…Recent advances in backscattered electron (BSE) technology have enabled researchers to examine the mineral content of bone independent of porosity on a microscopic scale (Vajda et al, 1995;Bloebaum et al, 1997;Roschger et al, 1998). The main objective of the present study was to begin the process of validating an animal model for examining the healing and mechanical properties of calcified fibrocartilage.…”

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confidence: 99%

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Experimental confirmation of the sheep model for studying the role of calcified fibrocartilage in hip fractures and tendon attachments

Shea¹,

Hallows²,

Bloebaum³

2002

Anat. Rec.

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Research has shown that there is a dramatic increase in the fractional area of calcified fibrocartilage from tendon and capsular insertions on the human femoral neck Shea et al., 2001b). Additional information regarding the properties of the proximal femur's cortical shell, gained from the use of an animal model, may result in a better understanding of elderly hip fracture since the cortical shell is a significant contributor to the strength of the proximal femur. The objective of the present study was to determine if the greater trochanter's tendon insertions of the human, rat, and sheep differ in terms of morphology and mineralization. The tendons of the greater trochanter of the human, rat, and sheep were observed to insert via a fibrocartilage insertion. The mineral content of the human and sheep calcified fibrocartilage was significantly higher than that of the rat calcified fibrocartilage (P Ͻ 0.01). Additionally, the mineral content of the rat cortical bone was significantly higher than that of the human cortical bone (P Ͻ 0.01). The mineral content of the calcified fibrocartilage and bone of the human and sheep were not statistically different from each other. There were also more similarities between the bone structure and lacunae density of the human and sheep than between the human and the rat. This suggests that the tendon insertions of the sheep are a better model than the tendon insertions of the rat for the investigation of calcified fibrocartilage in elderly hip fractures.

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“…Quantitative backscattered electron imaging was employed to determine the bone mineral density distribution (BMDD) according to the methodology described by Roschger et al [25]. Poly (methylmethacrylate) blocks used for bone histomorphometry were polished to a 1-μm finish with diamond particles, carbon-coated and observed with a scanning electron microscope (EVO LS10, Carl Zeiss Ltd, Nanterre, France) equipped with a five quadrant semi-conductor backscattered electron detector.…”

Section: Quantitative Backscattered Electron Imaging (Qbei)mentioning

confidence: 99%

Glucose-dependent insulinotropic polypeptide receptor deficiency leads to modifications of trabecular bone volume and quality in mice

Gaudin-Audrain¹,

Irwin²,

Mansur³

et al. 2013

Bone

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A role for the gastro-intestinal tract in controlling bone remodeling is suspected since serum levels of bone remodeling markers are affected rapidly after a meal. Glucose-dependent insulinotropic polypeptide (GIP) represents a suitable candidate in mediating this effect. The aim of the present study was to investigate the effect of total inhibition of GIP signaling on trabecular bone volume, microarchitecture and quality. We used GIP receptor (GIPR) knockout mice and investigated trabecular bone volume and microarchitecture by microCT and histomorphometry. GIPR-deficient animals at 16 weeks of age presented with a significant (20%) increase in trabecular bone mass accompanied by an increase (17%) in trabecular number. In addition, the number of osteoclasts and bone formation rate was significantly reduced and augmented, respectively in these animals when compared with wild-type littermates. These modifications of trabecular bone microarchitecture are linked to a remodeling in the expression pattern of adipokines in the GIPR-deficient mice. On the other hand, despite significant enhancement in bone volume, intrinsic mechanical properties of the bone matrix was reduced as well as the distribution of bone mineral density and the ratio of mature/immature collagen cross-links. Taken together, these results indicate an increase in trabecular bone volume in GIPR KO animals associated with a reduction in bone quality.

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Validation of quantitative backscattered electron imaging for the measurement of mineral density distribution in human bone biopsies

Cited by 403 publications

References 15 publications

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Experimental confirmation of the sheep model for studying the role of calcified fibrocartilage in hip fractures and tendon attachments

Glucose-dependent insulinotropic polypeptide receptor deficiency leads to modifications of trabecular bone volume and quality in mice

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