Validation of Pedigree Relationships Using a Multiplex Microsatellite Marker Assay in Iranian Holstein Cattle / Walidacja rodowodów irańskiego bydła holsztyńskiego przy użyciu analizy multipleks markerów mikrosatelitarnych
Abstract:in the present study, the pedigree and genotype data of 94 individuals were examined for accuracy of parentage allocations and identity test using 12 fluorescent-labelled microsatellite markers in a highly sensitive and accurate ABI system. The panel of 12 markers showed mean PIC value of 0.71 and Shannon index of 1.65 and 7.58 alleles per locus, which suggests that these markers are highly polymorphic and could be useful for parentage control. Based on scoring allele sizes, a total of 91 alleles were observed… Show more
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