VALIDATION OF MATRIX MATCHED CALIBRATION FOR ANALYSIS OF INSECTICIDE AND FUNGICID RESIDUES IN CUCUMBER AND TOMATO USING QuEChERS SAMPLE PREPARATION FOLLOWED BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Dashtbozorgi, Zahra; Ramezani, Mohammad Kazem; Husain, Syed Waqif; Abrumand-Azar, P.; Morowati, Mohsen

doi:10.4067/s0717-97072013000200012

Cited by 9 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method coupled with d‐SPE allows both polar and non‐polar compounds to be simultaneously extracted, in addition to being low cost, since low volumes of solvent are required. In addition, it is time saving since multiple classes of pesticides can be analyzed through a single run and higher recoveries are attained as compared to methods such as Luke's method which is time consuming and has high solvent demand (Anastassiades & Lehotay et al., 2003) Acetonitrile was preferred for extraction because it is the most common solvent used in multi‐residue extraction procedures, is miscible with water, promotes extraction in single phase, permits extraction of a wide range of pesticides with dissimilar polarities, and does not require addition of solvents (Dashtbozorgi et al., 2013). Acidification of extraction solvent (1% of acetic acid in acetonitrile) increased extraction efficiency, promoted adequate recoveries, minimal degradation, and hence assurance of method stability.…”

Section: Resultsmentioning

confidence: 99%

“…The working range was considered linear when recoveries of individual standards were between 70% and 120%, a regression coefficient ( r 2 ≥ 0.95) was observed, and adequate precision was achieved (<20%) (Granados‐Povedano et al., 2023). Detection and quantitation limits were based on standard deviations of the response of the standard blank and the slope of the calibration curve at signal‐to‐noise ratio of 3 and 10, respectively (Dashtbozorgi et al., 2013). Precision, expressed as percent standard deviation (%RSD), was obtained by extracting three replicates at three concentrations (10, 50, and 100 µg/L) of the studied pesticides in potato blank extracts, calculating standard deviation and dividing by the mean (Sapahin et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Currently, no multi‐residue approach is reported for the optimization of the modified QuEChERS extraction method for the detection of selected pesticides in potato matrices. This study, therefore, sought to optimize and validate the QuEChERS‐d‐SPE extraction procedure, combined with GC MS/MS, for real‐time quantification of four compounds of pesticides in different varieties of potatoes cultivated in Kenya (Dashtbozorgi et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of pesticide residues in potato varieties using modified quechers multi‐residue method combined with gas chromatography tandem mass spectrometric detection

Oyoo‐Ilukol,

Abong,

Okoth

et al. 2023

Crop Science

View full text Add to dashboard Cite

Regular monitoring of pesticide residues in potatoes (Solanum tuberosum L.) is important to ensure low risk of pesticide toxicity to consumers. An efficient, modified QuEChERS‐ d‐SPE was optimized and validated for quantification of pesticides residues (metalaxyl, chlorpyrifos, λ‐cyhalothrin, and cypermethrin) in popular Kenyan potato varieties ‘Shangi’, ‘Tigoni’ and ‘Dutch Robyjn’ grown under controlled field conditions using GC‐MS/MS. An evaluation of the effect of five spraying frequencies (Unsprayed, 5, 7, 10 and 14 days’ intervals) of metalaxyl‐based fungicides on residue levels in harvested potatoes was simultaneously done. Metalaxyl residue levels in the varieties were determined for each spraying regime. Method validation showed good linearity (r2) of 0.988‐0.999, detection limit (0.37 to 0.61 μg/L), quantification limit (1.20 to 1.84 μg/L) while recovery (71‐ 94%) and precision (% RSD of 2.3‐24.1%) were used to determine the accuracy of the method. Pyrethroids (cypermethrin and λ‐cyhalothrin) and organophosphates (metalaxyl and chlorpyrifos) were detected in the potato samples. Residue levels of the compounds were not significantly different (p = 0.05) between varieties except for chlorpyrifos. Cypermethrin was the residue compound detected at the highest mean level with 17.4 μg/L, followed by metalaxyl, chlorpyrifos and λ‐cyhalothrin, at 2.88, 1.05 and 0.15 μg/L respectively. Residue levels at different spraying frequencies varied statistically between varieties (p = 0.05). Dutch Robyjn sprayed at 7 days’ interval had the highest residue level of 9.49±0.26 μg/L. QuEChERS‐dSPE with GC‐MS/MS has shown suitability for routine pesticide residue monitoring in potatoes. The potatoes grown under the controlled experimental conditions did not present any concern for human health, as the pesticide residues detected were below Codex MRLs of 500 μg/L.This article is protected by copyright. All rights reserved

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of pesticide residues in potato varieties using modified quechers multi‐residue method combined with gas chromatography tandem mass spectrometric detection

Oyoo‐Ilukol,

Abong,

Okoth

et al. 2023

Crop Science

View full text Add to dashboard Cite

show abstract

“…The supervisory authorities have analyzed pesticides residue in various vegetable such as cucumber in around the world [7][8][9][10][11]. The present study is the first attempt for simultaneous determination 7 non-authorized pesticides in cucumber grown Darreh Shahr, according to use of these pesticides continuously in this city.…”

mentioning

confidence: 98%

Simultaneous Analysis of Seven Non-authorized Pesticides Residue in Cucumber Using Spiked Calibration Curve by GC/ECD

Yarinia

Amirahmadi

Ostadgholami

et al. 2017

IJT

View full text Add to dashboard Cite

show abstract

Simultaneous determination of multiclass pesticide residues in human plasma using a mini QuEChERS method

et al. 2017

View full text Add to dashboard Cite

Blood is one of the most assessable matrices for the determination of pesticide residue exposure in humans. Effective sample preparation/cleanup of biological samples is very important in the development of a sensitive, reproducible, and robust method. In the present study, a simple, cost-effective, and rapid gas chromatography-tandem mass spectrometry method has been developed and validated for simultaneous analysis of 31 multiclass (organophosphates, organochlorines, and synthetic pyrethroids) pesticide residues in human plasma by means of a mini QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. We have adopted a modified version of the QuEChERS method, which is primarily used for pesticide residue analysis in food commodities. The QuEChERS method was optimized by use of different extraction solvents and different amounts and combinations of salts and sorbents (primary-secondary amines and C) for the dispersive solid-phase extraction step. The results show that a combination of ethyl acetate with 2% acetic acid, magnesium sulfate (0.4 g), and solid-phase extraction for sample cleanup with primary-secondary amines (50 mg) per 1-mL volume of plasma is the most suitable for generating acceptable results with high recoveries for all multiclass pesticides from human plasma. The mean recovery ranged from 74% to 109% for all the analytes. The limit of quantification and limit of detection of the method ranged from 0.12 to 13.53 ng mL and from 0.04 to 4.10 ng mL respectively. The intraday precision and the interday precision of the method were 6% or less and 11% or less respectively. This method would be useful for the analysis of a wide range of pesticides of interest in a small volume of clinical and/or forensic samples to support biomonitoring and toxicological applications. Graphical Abstract Pesticide residues analysis in human plasma using mini QuEChERS method.

show abstract

VALIDATION OF MATRIX MATCHED CALIBRATION FOR ANALYSIS OF INSECTICIDE AND FUNGICID RESIDUES IN CUCUMBER AND TOMATO USING QuEChERS SAMPLE PREPARATION FOLLOWED BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Abstract: A rapid, specific and sensitive multi-residue method based on the quick, east, cheap, effective, rugged and safe (QuEChERS) has been developed and validated for the determination of 19 multi-class insecticide and fungicide residues (Aldrin

Cited by 9 publications

References 29 publications

Analysis of pesticide residues in potato varieties using modified quechers multi‐residue method combined with gas chromatography tandem mass spectrometric detection

Analysis of pesticide residues in potato varieties using modified quechers multi‐residue method combined with gas chromatography tandem mass spectrometric detection

Simultaneous Analysis of Seven Non-authorized Pesticides Residue in Cucumber Using Spiked Calibration Curve by GC/ECD

Simultaneous determination of multiclass pesticide residues in human plasma using a mini QuEChERS method

Contact Info

Product

Resources

About