Validation of liquid chromatography-tandem mass spectrometry for mevalonate in human plasma: Incompetent effects between treated atorvastatin & its combination with olmesartan in cardiovascular patients

Abstract

Aim: To develop a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method validation for the quantifi cation of mevalonate (MVA), an endogenous compound responsible for synthesis of cholesterol in human plasma. Settings and Design: The method was validated and impended for application on human to study the concentration of MVA in plasma level after administration of atorvastatin (ATVS) individually and with combination to olmesartan (OLM). Materials and Methods:The assay procedure involved the isolation of MVA from plasma samples using solid-phase extraction, preconditioned cartridge, washed with methanol followed by 0.1 N HCI. The analytes were eluted with 3 × 0.5 ml of methanol and evaporated to dryness Nitrogen stream. The residue was reconstitute for LCMS/MS analysis, were chromatographic separation was carried on a HyPurity advance, 50 × 4.6 mm column with a mobile phase 10 mM ammonium formate (pH = 8) and Acetonitrile. The fl ow rate was 0.8 ml/min throughout the process. The LC, Agilent 1290 coupled to electrospray ion mass spectrometer. Results: The developed method was validated for specifi city, accuracy, precision, stability, linearity, sensitivity, and recovery. The method was linear and found to be acceptable over the range of 50-1000 ng/ml. The method was successfully applied for the drug interaction study of ATVS + OLM by quantifying changes in levels of MVA in hypertensive patients. Conclusion: The study revealed concentration levels of MVA in ATVS + OLM compared to ATVS as single treated condition are nearly equal. This conclude that, MVA synthesize equal level of blood cholesterol on both the stage, but failed to reduce BP synergistically, that associate with vascular plague formation.

LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application

“…Data in the Multiple reaction monitoring (MRM) mode was considered, which showed better selectivity. 13 The positive ion spray mass spectrum revealed a protonated molecular by monitoring the transition pairs of m/z 223.1 precursor ion to the m/z 181.0 product ion for acetazolamide ( Figure 1a) and m/z 226.1 precursor ion to the m/z 182.2 product ion for the IS (Figure 1b).…”

Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form

“…2 Simultaneous administration of both ATC and ETB provide better control of plasma cholesterol due to synergistic and dual mechanism of action. 3 Different analytical methods are reported [4][5][6][7][8][9][10][11][12][13][14][15] for the analysis of both ATC and ETB including spectroscopic methods, HPLC, LCMS, CZE and micellar electro-kinetic capillary electrophoresis (MEKC) methods from formulations and biological samples. However, only one capillary electro phoresis method 9 has been reported for simultaneous determination of ATC and ETB, which suffer from a disadvantage like long analysis time (more than 15 min).…”

A novel and Rapid LC–MS/MS assay for the Determination of Mycophenolate and Mycophenolic Acid in Human Plasma

“…LC-MS methods are widely adopted in bioanalytical applications due to its specificity and high sensitivity. 18 For a bioavailability and bioequivalence studies, it is necessary to quantify the MP and MPA concentrations in in-vivo samples. 19 Till date, no LC-MS/MS method has been reported for the determination of MP individually or simultaneously with MPA in any of the biological matrices.…”