Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form

Abstract

Purpose: A simple, rapid, validated capillary zone electrophoresis (CZE) was established for the simultaneous estimation of atorvastatin and ezetimibe from solid dosage form. Method: The separation of both the drugs was accomplished on fused silica capillary (48cm × 50µmi.d.) using background electrolyte consisting of 2mM borate buffer (pH 9.3) and methanol (80:20 v/v) as BGE. All the capillary conditions were optimized and newly developed method was validated as per the ICH guidelines. Results: Good linear calibration was obtained for both the analytes ranging from 5 to 200µg/ml and 2 to 20 µg/ml for atorvastatin and ezetimibe respectively. The limit of detection and quantification were less than 2 µg/ml for both the analytes. Repeatability and intra-day precision were well within the acceptable range. The accuracy was expressed as percent recovery, which is found to be between 98.20% to 100.41%. Conclusion: The newly developed simple and validated CZE method was used for simultaneous determination of atorvastatin and ezetimibe from solid dosage form.Key words: CZE, atorvastatin, ezetimibe, validation, simultaneous determination Key Message: Newly developed analytical method is simple, rapid and validated, can be used for routine analysis of atorvastatin and ezetimibe, from formulations in pharmaceutical industries, clinical laboratories and analytical laboratories.

An Electrospun Nanofibrous Sensor Based on a Porous (Cr/Zn) Slats Oxide for Voltammetric Detection of Ezetimibe Drug in Real Samples

“…AlShehri obtained an LR of 6.04-120.9 μmol L À 1 for EZT in commercial medication via capillary electrophoresis (CE). As well, Attimarad M. obtained LR of to 20 μg/ml with LD of 0.60 μg/ml in solid dosage form via capillary electrophoresis (CE) [26]. However, this process is also expensive and presents certain limitations because it requires a specific type of laser to excite the specimen at a certain wavelength.…”

“…Given the therapeutic significance of EZT, numerous analytical techniques have been developed to evaluate its presence in body fluids [18][19][20][21][22][23][24][25][26][27]. However, these techniques present a number of critical drawbacks, including the requirement of expensive solvents and instruments for high-performance liquid chromatography.…”

Sensitive Spectrophotometric Determination of Atorvastatin in Pharmaceutical Formulation by Ion Pair Complexation with Pararosaniline Hydrochloride

“…Different techniques have been reported for the determination of ASN in pharmaceutical formulations and body fluids. These techniques involve HPLC with UV detection [2][3][4][5] , HPLC with fluorescence detection 6 , LC-MS/MS [7][8][9][10] , HPTLC [11][12][13] , voltametry [14][15][16] , capillary electrophoresis 17,18 , spectrophotometry [19][20][21] and spectrofluorometry 22 . Ion pair complexes or ion association complexes are complexes in which the species of analytical interest associates with oppositely charged ions to form a neutral extractable species 23 .…”

Determination on Vildagliptin in Rat Plasma by Capillary Electrophoresis Tandem Mass Spectrometry: It’s Application to Pharmacokinetic Study

“…Capillary electrophoresis is another highly efficient analytical technique used for quantitative analysis due to short analysis time, less consumption of solvent and less sample size. 20,21 Further, combination of CE with mass spectrometry provides added advantage such as high sensitivity and selectivity. Hence, in the present proposal simple, and sensitive CZE-ESI-QTOF-MS procedure was established for the estimation of VLG and applied for pharmacokinetic parameters determination from rats.…”