Determination on Vildagliptin in Rat Plasma by Capillary Electrophoresis Tandem Mass Spectrometry: It’s Application to Pharmacokinetic Study

Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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“…Attimarad et al. presented a method for the determination of vildagliptin in rat plasma including its application to a pharmacokinetic study .…”

Section: Analytical Applicationsmentioning

confidence: 99%

“…CZE-MS offered higher selectivity than MEKC-MS with LODs comparable to those obtained with LC methods. Attimarad et al presented a method for the determination of vildagliptin in rat plasma including its application to a pharmacokinetic study [172].…”

Section: Pharmaceutical and Clinical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…Different analytical methods have been described for the quantification of VILD from biological samples, including ultraperformance liquid chromatography-mass spectrometry (UPLC-MS/MS) [3], high-performance liquid chromatography (HPLC) [4], atomic absorption and emission spectrometry [5], capillary electrophoresis (CE-MS/MS) [6], LC-MS/MS [7,8], and hydrophilic interaction chromatography (HILIC/MS/MS) [9] methods, as well as in commercial formulations, including gas chromatography (GC-MS) [10], ultraviolet (UV) spectrophotometry [11][12][13][14], HPLC [15][16][17], reversed-phase (RP-HPLC) [18][19][20], and UV-HPLC [21] methods. Even though these chromatographic methods show high sensitivity and accuracy, they have several disadvantages such as the need for more laborious pretreatment of the sample in some cases, a long analysis time, and the use of highly toxic reagents or organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric quantitative analysis of vildagliptin in bulk form and spiked human serum at a modified electrode

et al. 2023

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The electrochemical behavior of Vildagliptin (VILD) was studied using the cyclic voltammetric technique in an aqueous Britton–Robinson (BR) universal buffer solution of various pH levels between 4.0 and 10 at a 5% calcium-montmorillonite clay modified with carbon paste electrode surface (5% Ca-MMT/CPE). The results exhibited an irreversible anodic peak at about 1.238 V versus Ag/AgCl, KCl (3 mol L−1). The anodic peak was found to be diffusion–adsorption controlled. The possible reaction mechanism is estimated taking into consideration of the calculated electrons and protons number transferred on the electrode/electrolyte interface using the cyclic voltammetric technique. VILD was found to adsorb onto the surface of 5% Ca-MMT/CPE in a monolayer surface coverage of 3.0 × 10−12 mol cm−2. A validated square wave voltammetry (SWV) technique for VILD determination was performed. The calibration curve of VILD onto the 5% Ca-MMT/CPE surface was linear in the concentration range of 1.0–110 nmol L−1 with the mean limits of detection and quantification was 0.285 and 0.950 nmol L−1, respectively, in the bulk form. The proposed procedure for the assay of VILD in bulk form, dosage form, and spiked human serum has the advantage of being simple, rapid, sensitive, and inexpensive compared to other analytical methods. The described method showed an excellent performance for the trace determination of VILD in its formulation without interference from excipients. Graphical abstract

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“…Vildagliptin has shown good glycemic control with a reduced threat of hypoglycemic effect and increase in body weight, along with a protective effect on the heart [ 14 , 15 , 16 , 17 , 18 ]. Many assay procedures have been announced for the analysis of VD, such as spectrophotometric [ 19 , 20 , 21 ], spectrofluorometric [ 21 ], HPTLC [ 22 ], capillary electrophoresis [ 23 ], LC–MS/MS [ 24 ], and electrochemical [ 25 ] methods.…”

Section: Introductionmentioning

confidence: 99%

An Experimental Design Approach to Quantitative Expression for Quality Control of a Multicomponent Antidiabetic Formulation by the HILIC Method

et al. 2022

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A rapid and reproducible hydrophilic liquid chromatography (HILIC) process was established for concomitant determination of remogliflozin etabonate (RE), vildagliptin (VD), and metformin (MF) in a formulation. A face-centered central composite experimental design was employed to optimize and predict the chromatographic condition by statistically studying the surface response model and design space with desirability close to one. A HILIC column with a simple mobile phase of acetonitrile (65% v/v) and 20 mM phosphate buffer (35% v/v, pH 6, controlled with orthophosphoric acid) was used to separate RE, VD, and MF. RE, VD, and MF were separated in 3.6 min using an isocratic mode mobile phase flow at a flow rate of 1.4 mL at room temperature, and the analytes were examined by recording the absorption at 210 nm. The developed HILIC method was thoroughly validated for all parameters recommended by ICH, and linearity was observed in the ranges 20–150 µg/mL, 10–75 µg/mL, and 50–750 µg/mL for RE, VD, and MF, respectively, along with excellent regression coefficients (r2 > 0.999). The calculated percentage relative deviation and relative error ascertained the precision and accuracy of the method. The selectivity and accuracy were further confirmed by the high percentage recovery of added standard drugs to the formulation using the standard addition technique. The robustness of the HILIC processes was confirmed by developing a half-normal probability plot and Pareto chart, as the slight variation of a single factor had no significant influence on the assay outcomes. Utilization of the optimized HILIC procedure for concurrent quantification of RE, VD, and MF in solid dosage forms showed accurate and reproducible results. Hence, the fast HILIC method can be regularly employed for the quality assurance of pharmaceutical preparations comprising RE, VD, and MF.

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Determination on Vildagliptin in Rat Plasma by Capillary Electrophoresis Tandem Mass Spectrometry: It’s Application to Pharmacokinetic Study

Cited by 11 publications

References 19 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Voltammetric quantitative analysis of vildagliptin in bulk form and spiked human serum at a modified electrode

An Experimental Design Approach to Quantitative Expression for Quality Control of a Multicomponent Antidiabetic Formulation by the HILIC Method

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