Validation of immunity induced by inactivated CCPP vaccine with different adjuvants

Ayelet, Gelagay; Yigezu, Laekemariam; Aschalew, Zeleke; Gelaye, Esayas; Asmare, Kassahun

doi:10.1016/j.smallrumres.2007.02.004

Cited by 10 publications

(17 citation statements)

References 8 publications

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“…Vaccination remains to be the most costeffective measure for preventing and reducing the severity of infectious diseases like CCPP in the goat population. Past trials have demonstrated that immunization of goats with inactivated CCPP vaccine confers protective immunity; however, all of those trials used approximately equal aged goats for their studies (Rurangirwa et al, 1991;Ayelet et al, 2007;Tarekegn et al, 2012). Hence, further investigation is needed regarding the effectiveness of currently used CCPP vaccine in all (various) age groups of goat population.…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of contagious caprine pleuropneumonia and field performance of inactivated vaccine in Borana pastoral area, southern Ethiopia

Lakew¹,

Sisay²,

Ayelet³

et al. 2014

Afr. J. Microbiol. Res.

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This study was conducted between September 2012 and May 2013 in three Districts of Borana pastoral area to determine seroprevalence of contagious caprine pleuropneumonia (CCPP) and assess field performance of inactivated commercial vaccine, produced by the National Veterinary Institute (NVI) in Ethiopia, against CCPP. Both pre and post vaccination sera samples were tested using competitive enzyme linked immunosorbent assay (cELISA). Out of 510 examined sera, 161 samples were positive for CCPP, giving an overall seroprevalence of 31.6% (95% CI = 27.57-35.64%) in the study area. Seroprevalence of 35.2, 35.1 and 25% were recorded in Arero, Dhas and Yabello districts, respectively. However, there was no significant difference (χ2=5.56, P=0.062) in seropositivity among the three districts and between male and female goats (χ2=0.068, P=0.794) examined in this study. On the other hand, the differences in seroprevalence among the age categories were statistically significant (χ2=24.48, p<0.0001). A rise in antibody (seroconversion) was observed after field vaccination of goats with inactivated CCPP vaccine and a total of 253 of the 414 examined sera samples were positive for Mycoplasma capricolum subsp. capripneumoniae (Mccp) specific antibodies, thus 61.1% of goats seroconverted following vaccination. Comparison of Mccp specific antibodies in the goat population before and after vaccination indicated that the number of positive reactors increased significantly (P<0.0001) following CCPP vaccination. Seropositivity following vaccination was analyzed among the three age groups and statistically significant differences (χ2=45.48, P<0.0001) were recorded. The change in the serum antibody after vaccination was found to be higher in younger and adult aged goats than old aged goats. In conclusion, the present study indicates that CCPP is one of the major goat health problems in Borana pastoral area which warrants appropriate measures to be in place towards the prevention and control of the disease. Moreover, field vaccination of goats by inactivated CCPP vaccine induced seropositivity in majority of the inoculated goats. Future controlled experimental studies with challenge infection after vaccination need to be conducted for further evaluation of the vaccine efficacy.

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Section: Discussionmentioning

confidence: 99%

“…Mccp (F-38 Kenyan strain) in adjuvant confers protection against a contact challenge (Ayelet et al, 2007). However, the efficacy of inactivated Mccp vaccine under field conditions has not been clearly documented.…”

Section: Experimental Trials Conducted In the Country Have Demonstratmentioning

confidence: 99%

Seroprevalence of contagious caprine pleuropneumonia and field performance of inactivated vaccine in Borana pastoral area, southern Ethiopia

Lakew¹,

Sisay²,

Ayelet³

et al. 2014

Afr. J. Microbiol. Res.

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“…CCPP is a rampant and highly contagious animal disease with potential of rapid spread irrespective of national borders (Gelagay et al 2007). Mycoplasma capricolum subsp.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of contagious caprine pleuropneumonia in Kefta Humera, Alamata (Tigray) and Aba-‘ala (Afar), Northern Ethiopia

Hadush

Eshetu

Mengistu

et al. 2008

Trop Anim Health Prod

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A cross sectional study was conducted to determine the sero-prevalence of contagious caprine pleuroneumonia in three districts of Tigray and Afar regions of Ethiopia namely; Kefta Humera, Alamata and Aba-'alla. Proportions and chi-square test statistics were used to analyze the data. From a total of 863 goats and 137 sheep tested, 282 (32.68%) and 25 (18.25%) were positive for antibodies of Mycoplasma capricolum subsp. capripneumoniae respectively using complement fixation test (CFT). The seroprevalence of CCPP in goats among the three districts was statistically significant (x 2 =76.00, p<0.001). In this study there was no statistical significant variation in the seroprevalence of CCPP in both sexes (x 2 =3.619, p=0.0571) and age (x 2 =0.990, p=0.095) groups. The finding of high seroprevalence of CCPP in sheep (18.25%) could indicate that sheep are potential carriers of Mccp.

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“…Statistically was performed by using experimental M. haemolytica serotype A2, A7, and A2-A7 combined vaccine, grown under iron restriction, in four trial groups of lambs followed by challenge with homologous serotype of M. haemolytica. The Mannheimia serotypes contained in this experimental vaccine appear to be appropriate for Ethiopian conditions, because they have been shown to occur at a high frequency in different parts of the country (Zeleke, 1998;Teferi, 2000;Sisay and Zerihun, 2003;Ayelet et al, 2004;Abera, 2005;Mulate, 2007) and did not have a vaccine produced in Ethiopia against these serotype. The most important result of this study was that vaccination with A2 and A7 M. haemolytica combined vaccine confer relatively good protective efficacy than M. haemolytica A7 or A2 vaccinated groups.…”

Section: Discussionmentioning

confidence: 99%

“…M. haemolytica serotypes A2 and A7 were selected and used as candidate vaccine strains based on their higher rate of isolation from different parts of Ethiopia (Zeleke, 1998;Teferi, 2000;Sisay and Zerihun, 2003;Ayelet et al, 2004;Mulate, 2007). These strains were isolated and identified from different outbreaks of pneumonic pasteurellosis in the country based on sugar fermentation (arabinose and trehalose) and the organisms were serotyped based on capsular antigens using indirect haemagglutination test with M. haemolytica known serotype antisera and stored at NVI germ bank in a lyophilized formed at -20°C.…”

Section: Experimental Vaccines and Adjuvant Preparationmentioning

confidence: 99%

Efficacy of Mannheimia haemolytica A2, A7, and A2 and A7 combined expressing iron regulated outer membrane protein as a vaccine against intratracheal challenge exposure in sheep

Tesfaw¹,

Jenberie²,

Sori³

et al. 2014

Afr. J. Microbiol. Res.

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This experimental study was done on a total of 40 male lambs with the objectives of developing experimental vaccines from Mannheimia haemolytica serotypes A2 and A7 that express iron regulated outer membrane protein and in vivo evaluation of their efficacy. Lambs were categorized in to four experimental groups and vaccinated with 1 ml of vaccine containing 5 × 10 8 CFU/ml. Group 1 was vaccinated with M. haemolytica A2, group 2 with A7, group 3 with serotype A2 and A7 combination, and group 4 received saline as control. They were challenged intratracheally by the respective homologous serotype after 35 days of vaccination. Post challenge clinical investigation showed that significant higher rate of morbidity was seen in control group which was demonstrated by raised rectal temperature (by 0.5-1°C) and respiratory signs. From the total of 26 lambs challenged with live M. haemolytica A2 and A7, 6 (23.1%) and 4 (15.3%) lambs were found dead and sick, respectively. Higher mortality and morbidity were observed in unvaccinated control group; however, lesser was recorded in combined vaccinated group. Lung lesions of variable severity were observed in 13 (50.0%) lambs following challenge. From vaccinated groups, 5 (27.8%) lambs were found to have a +1 lung lesion score. All of the lambs in unvaccinated control group had scores between +2 and +3. There was a statistically significant difference (p < 0.05) between control and vaccinated groups, while no statistically significant difference (p > 0.05) was seen among vaccinated groups concerning lung lesion scores. Furthermore, the respective serotypes of M. haemolytica were successfully re-isolated from pneumonic lungs at a mean titre range of 10 2.2 -10 8.1 CFU/g. In conclusion, lambs which received combined vaccine confer relatively good protective efficacy than M. haemolytica A2 or A7 vaccinated groups. Therefore, further study should be done on evaluation of antibody titer at different time points.

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Validation of immunity induced by inactivated CCPP vaccine with different adjuvants

Cited by 10 publications

References 8 publications

Seroprevalence of contagious caprine pleuropneumonia and field performance of inactivated vaccine in Borana pastoral area, southern Ethiopia

Seroprevalence of contagious caprine pleuropneumonia and field performance of inactivated vaccine in Borana pastoral area, southern Ethiopia

Seroprevalence of contagious caprine pleuropneumonia in Kefta Humera, Alamata (Tigray) and Aba-‘ala (Afar), Northern Ethiopia

Efficacy of Mannheimia haemolytica A2, A7, and A2 and A7 combined expressing iron regulated outer membrane protein as a vaccine against intratracheal challenge exposure in sheep

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