Validation of Gender Specific CAPS Marker in Turkish Fig (Ficus carica L.) Collection and F1 Progenies

Ikten, Hatice; Yılmaz, Y.

doi:10.15835/nbha47311499

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…FcRAN1 was preliminarily speculated to be the sex-determining A gene for figs, and a cleaved amplified polymorphism sequence (CAPS)-based method was established . Ikten and Yilmaz (2019) verified the CAPS method and found that restriction endonuclease PciI provides 100% sex discrimination for PCR products, while restriction endonuclease HpyCH4IV gave some false-positive results. Nevertheless, restriction endonuclease digestion is timeconsuming, the required enzymes are expensive, and wellcontrolled conditions are needed for successful performance.…”

Section: Data Availability Statementmentioning

confidence: 90%

Breeding Innovations in Underutilized Temperate Fruit Trees

2022

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation.

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Section: Data Availability Statementmentioning

confidence: 90%

Breeding Innovations in Underutilized Temperate Fruit Trees

2022

Frontiers Research Topics

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“…FcRAN1 was preliminarily speculated to be the sex-determining A gene for figs, and a cleaved amplified polymorphism sequence (CAPS)-based method was established ( Mori et al, 2017 ). Ikten and Yilmaz (2019) verified the CAPS method and found that restriction endonuclease Pci I provides 100% sex discrimination for PCR products, while restriction endonuclease Hpy CH4IV gave some false-positive results. Nevertheless, restriction endonuclease digestion is time-consuming, the required enzymes are expensive, and well-controlled conditions are needed for successful performance.…”

Section: Introductionmentioning

confidence: 90%

AGAMOUS Gene as a New Sex-Identification Marker in Fig (Ficus carica L.) Is More Efficient Than RAN1

Song

Flaishman

et al. 2021

Front. Plant Sci.

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Fig is an ancient gynodioecious fruit tree with females for commercial fruit production and hermaphrodites (males) sometimes used as pollen providers. An early sex-identification method would improve breeding efficiency. Three AGAMOUS (AG) genes were recruited from the Ficus carica genome using AG sequences from Ficus microcarpa and Ficus hispida. FcAG was 5230 bp in length, with 7 exons and 6 introns, and a 744-bp coding sequence. The gene was present in both female and male fig genomes, with a 15-bp deletion in the 7th exon. The other two AG genes (FcAG2-Gall_Stamen and FcAG3-Gall_Stamen) were male-specific, without the 15-bp deletion (759-bp coding sequence), and were only expressed in the gall and stamen of the male fig fruit. Using the deletion as the forward primer (AG-Marker), male plants were very efficiently identified by the presence of a 146-bp PCR product. The previously reported fig male and female polymorphism gene RESPONSIVE-TO-ANTAGONIST1 (RAN1) was also cloned and compared between male and female plants. Fifteen SNPs were found in the 3015-bp protein-coding sequence. Among them, 12 SNPs were identified as having sex-differentiating capacity by checking the sequences of 27 known male and 24 known female cultivars. A RAN1-Marker of 608 bp, including 6 SNPs, was designed, and a PCR and sequencing-based method was verified with 352 fig seedlings from two hybrid populations. Our results confirmed that the newly established AG-Marker is as accurate as the RAN1-Marker, and provide new clues to understanding Ficus sex determination.

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“…Mori et al (2017) reported that mutational variations of the two SNP regions in RAN1, which is associated with sex determination in figs, caused heterozygous accessions in the 18 male fig accessions. In the current study, five accessions previously reported to be female (Ikten and Yilmaz, 2019) were identified as male based on RAN1associated cleaved amplified polymorphic sequences (CAPS) marker screenings. These heterozygous genome variations may be another reason for the fact that 5 of the 45 caprifigs accessions were found in different RPPs.…”

Section: Unique Synonymous-clonal Structure and Sex Distribution Of Anatolian Figsmentioning

confidence: 99%

Genetic characterisation and population structure analysis of Anatolian figs (Ficus carica L.) by SSR markers

Ergül

Büyük

Hazrati

et al. 2021

Folia Horticulturae

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The common fig (Ficus carica L.) is a tree species and is one of the oldest fruit trees cultivated in Turkey. The Western Anatolian region of Turkey produces nearly a quarter of the total dried fig production of the world. This region also harbours a rich fig germplasm. However, so far this germplasm has remained largely uncharacterised. In this study, using 14 simple sequence repeat (SSR) primer pairs, we analysed a total of 310 fig accessions from six different regions of Anatolia. In structure analyses, Western Anatolian accessions formed a group, which was correlated with their geographical distribution. In addition, 7 identical, 36 synonymous, and 22 homonymous fig accessions were identified. In multilocus lineages (MLLs) analysis a total of 54 accessions were matched to different accessions as clone assignment. The results will facilitate future germplasm management and breeding efforts in this economically important tree species by identifying genetic diversity, genetic relations and characterising the structure of studied populations and accessions.

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Validation of Gender Specific CAPS Marker in Turkish Fig (Ficus carica L.) Collection and F1 Progenies

Cited by 5 publications

References 17 publications

Breeding Innovations in Underutilized Temperate Fruit Trees

Breeding Innovations in Underutilized Temperate Fruit Trees

AGAMOUS Gene as a New Sex-Identification Marker in Fig (Ficus carica L.) Is More Efficient Than RAN1

Genetic characterisation and population structure analysis of Anatolian figs (Ficus carica L.) by SSR markers

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