Validation of five X-chromosomal STRs DXS6800, DXS6807, DXS6798, DXS8377 and DXS7423 in an Antioquian population sample

Moreno, Miguel Eduardo Torres; Builes, J.J.; Jaramillo, Paula; Espinal, C.; Aguirre, D.; Pancorbo, Marian M. de; Gusmão, Leonor; Bravo, M.L.J.

doi:10.1016/j.ics.2005.09.093

Cited by 5 publications

(4 citation statements)

References 12 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The allele frequency distributions in our sample were compared with data available for the same markers studied in other populations from the same continent [9,11,12,15] and also with data available for the same 10 STRs in a Spanish sample [1] because Spain was one of the main sources of emigration that contributed to the Colombian gene pool. Although available data on other South American populations are scarce, in the few possible comparisons, low, nonsignificant genetic distances were observed (see Table S1).…”

Section: Population Comparisonsmentioning

confidence: 99%

See 1 more Smart Citation

Genetic profile characterization and segregation analysis of 10 X-STRs in a sample from Santander, Colombia

et al. 2008

View full text Add to dashboard Cite

Ten X-chromosome short tandem repeats (X-STRs: DXS8378, DXS7132, DXS9898, DXS6809, DXS6789, DXS101, GATA172D05, HPRTB, DXS8377, and DXS7423) were analyzed in a sample of unrelated individuals (108 males and 110 females) from the Santander Department in Colombia. In this sample, gene diversities varied between 63.56%, for DXS8378, and 91.41%, for DXS8377. For this set of 10 X-STRs, a high discrimination power was obtained for both male (1 in 3 x 10(6)) and female (1 in 9 x 10(10)) samples and a high mean exclusion chance in father/daughter duos (99.993%) and in father/mother/daughter trios (99.9999%), demonstrating the usefulness of this set of markers in forensic and kinship analysis. Hardy-Weinberg equilibrium was tested in the female sample and no significant deviations were found. Pairwise analysis showed significant differences in the comparison with samples from Spain, Peru, and Argentina and with African American and Hispanic samples from New York. This same set of X-STRs was also typed in 51 mother/father/daughter trios, 43 mother/son duos, and in a single father/daughter pair. In total, four mutations were observed; one at DXS7132 and at DXS6809, and two at DXS8377. Two mutations were paternal and one maternal; and to a fourth mutation, it was not possible to define its origin.

show abstract

Section: Population Comparisonsmentioning

confidence: 99%

“…Nevertheless, further studies are still needed on allele frequency distributions in different populations, mutation rates, and linkage disequilibrium to establish reference population databases [2,12,15]. In the case of X-STRs, it is particularly important to screen possible associations between loci (linkage disequilibrium).…”

Section: Introductionmentioning

confidence: 99%

Genetic profile characterization and segregation analysis of 10 X-STRs in a sample from Santander, Colombia

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The X-chromosome analysis was carried out by using the Genephile X-Plex Human X Chromosome STR mapping set (GenePhile Bioscience Co., Ltd., Taipei, Taiwan), which selectively amplified 13 X-chromosome STR loci (DXS8378, DXS9898, DXS8377, HPRTB, GATA172D05, DXS7423, DXS6809, DXS7132, DXS101, DXS6789, DXS9902, DXS6807 and DXS7424). These STR loci have been previously evaluated for forensic purpose by many workers [13][14][15][16] and the result is presented in Table 2. Out of 13 loci tested, a complete match of all the 13 Xchromosome loci in the questioned child with the mother was observed.…”

Section: Resultsmentioning

confidence: 99%

False paternity with one or two mismatches using commercial STR kits

Akhteruzzaman

Majumder

Ferdous

et al. 2012

Australian Journal of Forensic Sciences

View full text Add to dashboard Cite

“…Finally, the X-chromosome analysis was carried out by using Investigator ® Argus X-12 PCR kit (Qiagen, Germany) at 12 loci. These STRs loci have been evaluated for forensic purpose by many different groups (Szibor et al, 2000;Moreno et al, 2006;Turrina et al, 2007& Gomes et al, 2009.…”

Section: Pcr Amplificationmentioning

confidence: 99%

A case study on limitations of commercial autosomal STR kits for determination of half-sibling by DNA analysis

Hasan

Sharmin

Akhteruzzaman

2016

Asian Australas. J. Biosci. Biotechnol.

View full text Add to dashboard Cite

A case study was conducted to evaluate the significance of adding lineage markers for a complex kinship analysis. Additional genetic information may increase likelihood ratio values for true relationships in a pedigree, while reducing the chance of identifying false relationships. It is very difficult to establish a relationship between siblings by the statistical evaluation with autosomal STR markers. The clear discrimination of true versus false relationships is important for complex kinship cases, such as sibling testing, deficient paternity testing, missing person’s identification as well as immigration testing with the help of forensic DNA technology. This case study represents a false parent-child relation or full sibling, one individual is male and other individual is female, where matches were found at least one allele at all 23 tested autosomal STR loci with Combined Likelihood is 75,446,930.822 for Parent-Child and 284,041.488 for Half-Sibling indicates that 266 times more likely parent-child than half-sibling. In order to reach an accurate conclusion, we further included one sister of the putative brother and mother of the putative sister and these four individuals DNA samples were analyzed with X-chromosome STR kits, showing mismatches between claimed siblings in eight loci out of twelve loci and confirm that they are not parent-child. Finally we confirm that they are half-sibling and they are come from same father but different mother. This case emphasizes the necessity of lineage markers like X chromosome or Y chromosome DNA data for interpreting critical sibling cases where one of the siblings is female and their father is dead. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 148-151

show abstract

Validation of five X-chromosomal STRs DXS6800, DXS6807, DXS6798, DXS8377 and DXS7423 in an Antioquian population sample

Cited by 5 publications

References 12 publications

Genetic profile characterization and segregation analysis of 10 X-STRs in a sample from Santander, Colombia

Genetic profile characterization and segregation analysis of 10 X-STRs in a sample from Santander, Colombia

False paternity with one or two mismatches using commercial STR kits

A case study on limitations of commercial autosomal STR kits for determination of half-sibling by DNA analysis

Contact Info

Product

Resources

About