Validation of Comet assay in Oregon-R and Wild type strains of Drosophila melanogaster exposed to a natural radioactive environment in Brazilian semiarid region

Verçosa, Cícero Jorge; Filho, Aroldo Vieira de Moraes; Castro, Ícaro Fillipe de Araújo; Santos, Robson Gomes dos; Cunha, Kênya Silva; Silva, Daniela de Melo e; Garcia, Ana Cristina Lauer; Navoni, Júlio Alejandro; Amaral, Viviane Souza do; Rohde, Cláudia

doi:10.1016/j.ecoenv.2017.03.024

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…D . melanogaster had been already efficiently used for biological studies as an animal model for non-human natural background dosimetry [ 20 , 51 ]. Additionally, it was shown that the fruit fly is a suitable organism for studies in deep underground low-background laboratories for which the effect of low-radiation background was observed after two weeks of exposition [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

et al. 2021

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Natural background radiation is a permanent multicomponent factor. It has an influence on biological organisms, but effects of its deprivation still remain unclear. The aim of our work was to study for the first time responses of D. melanogaster to conditions of the Deep Underground Low-Background Laboratory DULB-4900 (BNO, INR, RAS, Russia) at the transcriptome level by RNA-seq profiling. Overall 77 transcripts demonstrated differential abundance between flies exposed to low and natural background radiation. Enriched biological process functional categories were established for all genes with differential expression. The results showed down-regulation of primary metabolic processes and up-regulation of both the immune system process and the response to stimuli. The comparative analysis of our data and publicly available transcriptome data on D. melanogaster exposed to low and high doses of ionizing radiation did not reveal common DEGs in them. We hypothesize that the observed changes in gene expression can be explained by the influence of the underground conditions in DULB-4900, in particular, by the lack of stimuli. Thus, our study challenges the validity of the LNT model for the region of background radiation doses below a certain level (~16.4 nGy h-1) and the presence of a dose threshold for D. melanogaster.

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Section: Discussionmentioning

confidence: 99%

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

et al. 2021

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“…The genotoxic effect of the different concentrations of the dry decoction of M. urundeuva leaves was assessed using the comet assay in somatic D. melanogaster cells according to Verçosa et al () with slight modifications. Adult Oregon‐R D. melanogaster females were transferred to glass vials for oviposition.…”

Section: Methodsmentioning

confidence: 99%

“…Next, larvae were transferred to a Kline concavity slide containing ethylenediamine tetraacetic acid (EDTA) to prevent hemolymph coagulation. A section was cut on the side of the cuticle of each larva using a scalpel to remove hemolymph, according to Verçosa et al (). A 60‐μL cell‐suspension containing the pooled hemocytes of the 60 larvae was mounted on glass slides previously sanded and soaked in standard agarose 1.5%.…”

Section: Methodsmentioning

confidence: 99%

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

Amorim

Santana

Silva

et al. 2019

Environ and Mol Mutagen

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Medicinal plants are worldwide used as an efficient treatment of many diseases. Myracrodruon urundeuva Allemão (Anacardiaceae) is widely used Brazilian folk medicine to treat inflammations and infections of the female genital tract, conditions of the stomach and throat, and to heal wounds on the skin and mucous membranes. Several pharmacological properties of extracts and compounds isolated from M. urundeuva are found in the literature, corroborating its uses as antiulcer and gastroprotective, anti‐inflammatory and analgesic, as well as antimicrobial. Despite these many uses in traditional herbal medicine, there are few reports of its toxic‐genetic effect. This work aimed to investigate the genotoxic and mutagenic potential in vivo of the dry decoction of M. urundeuva leaves on somatic cells of Drosophila melanogaster, through the Comet assay and somatic mutation and recombination test (SMART). Six concentrations (0.5, 1.0, 2.0, 4.0, 8.0, and 16.0 mg/mL) were studied after feeding individuals for 24 hr in culture medium hydrated with extracts of M. urundeuva. In the Comet assay, all concentrations showed a genotoxic effect significantly higher than the negative control group, treated with distilled water. The two highest concentrations were also superior to the positive control group, treated with cyclophosphamide (1 mg/mL). In the SMART, there was a mutagenic effect at all concentrations tested, with a clear dose‐dependent relationship. Both recombination and mutation account for these mutagenic effects. The set of results indicate that the dry decoction of M. urundeuva leaves is genotoxic and mutagenic for D. melanogaster under the experimental conditions of this study. Environ. Mol. Mutagen. 61:329–337, 2020. © 2019 Wiley Periodicals, Inc.

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“…The comet assay has been applied to several insect species belonging to various systematic groups and inhabiting different ecological niches. The most often used insect in DNA damage research is undoubtedly D. melanogaster [21,[309][310][311][312][313], although there are studies conducted also on Drosophila simulans [314]. The presence of numerous repair deficient/efficient mutants of D. melanogaster, allows for the design of complex experimental models that can be used to understand DNA repair mechanisms [25] and D. melanogaster was successfully developed as a model organism in toxicological studies [315]; a new term "Drosophotoxicology" was proposed [316].…”

Section: Insectsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Validation of Comet assay in Oregon-R and Wild type strains of Drosophila melanogaster exposed to a natural radioactive environment in Brazilian semiarid region

Cited by 12 publications

References 25 publications

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

Genotoxic Assessment of the Dry Decoction of Myracrodruon urundeuva Allemão (Anacardiaceae) Leaves in Somatic Cells of Drosophila melanogaster by the Comet and SMART Assays

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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