“…The flow rate of the mobile phase was maintained at 1.0 mL/min. Injection volume was 50 µL and the detector wavelengths were set at 290 nm and 440 nm as excitation and emission, respectively (Giorgi et al, 2011). Briefly, the quantification of tepoxalin and RWJ-20142 in plasma samples was accomplished by chromatographic analysis of unknown samples in parallel with standard curve and quality control samples created using tepoxalin, RWJ-20142 and RWJ-20294 (internal standard) pure standard powders (TopChem Laboratories).…”