Validation of a spectrophotometric method for the determination of paracetamol in plasma applicable for toxicological emergencies in laboratories with limited resources

Chefirat, Bilel; Zergui, Anissa; Belmessabih, Meriem Nour; Rahmani, Chaïmaa; Rezk-kallah, Haciba

doi:10.1016/j.toxac.2020.07.003

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…[8] Numerous methods have been used for the determination of paracetamol in pharmaceutical formulations and biological fluids including UV-vis spectrophotometry, fluorimetry, near infrared transmittance spectroscopy and chromatography with several detectors. [9][10][11][12][13] The quantification of PAR from in vitro dissolution assays of commercially available formulations has been carried out with spectrophotometric methods. [14][15][16] Electrochemical drug determination has attracted great attention because electrochemical methods are low cost, highly sensitive, easy to use, and portable when needed.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotube Modified Glassy Carbon Electrodes for on‐line and off‐line in vitro Therapeutic Equivalence Assessment of Paracetamol Tablets

Cumsille‐Escandar,

Yáñez,

Álvarez‐Lueje

2024

ChemistrySelect

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In vitro bioequivalence assessment of paracetamol tablets was carried out by differential pulse voltammetry (DPV) and amperometry using a multiwalled carbon nanotube modified glassy carbon electrode (GCE/MWCNT). For the DPV developed method, samples were collected from the USP paddle apparatus, while amperometric measurements were carried out in situ. The DPV method was validated in three supporting electrolytes (per in vitro bioequivalence guidelines) by measuring the recovery from standard solutions and paracetamol tablet solutions, while precision was evaluated through the RSD (n=6). In HCl pH 1.2 solution the LOD and LOQ were 48 nM and 160 nM, respectively and the linear range was between 0.18–2.2 μM. In acetate buffer pH 4.5 the LOD and LOQ were 15 nM and 51 nM, respectively and the linear range was between 88 nM–880 nM. And in phosphate buffer pH 6.8 the LOD and LOQ were 4.6 nM and 15 nM, respectively and the linear range was between 88 nM–880 nM. Recovery values were between 95 and 105 % and precision values were less than 5 %. Results showed that both methods were effectively applied for the analysis of samples.

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Section: Introductionmentioning

confidence: 99%

Carbon Nanotube Modified Glassy Carbon Electrodes for on‐line and off‐line in vitro Therapeutic Equivalence Assessment of Paracetamol Tablets

Cumsille‐Escandar,

Yáñez,

Álvarez‐Lueje

2024

ChemistrySelect

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“…In the literature, many quantitative analytical methods have been investigated in order to the separate determination of tramadol and paracetamol in the drug industry. PAR in samples was analysed by several analytical methods including UV-spectrophotometry [ 11 , 12 ], HPLC [ 13 – 16 ], gas chromatography-tandem mass spectrometry [ 17 ], electrochemistry [ 18 – 19 ], capillary electrophoresis [ 20 ] and Fourier transform infrared spectroscopy (FTIR) [ 21 , 22 ]. The presence of tramadol can be determined in a variety of pharmaceutical products and biological materials through the use of certain analytical techniques, the use of which has been thoroughly researched and documented using UV-spectrophotometry [ 23 – 25 ], fluorescence spectroscopy [ 26 ], liquid chromatography-tandem mass spectrometry [ 27 ] gas chromatography-mass spectrometry (GC-MS) [ 28 ], capillary electrophoresis with electrochemiluminescence (CE-CL) [ 29 ], FT-Raman [ 30 ], voltammetry [ 31 ], can be found in the literature.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric quantification of paracetamol and tramadol hydrochloride by chemometric calibration methods

SELİMOĞLU,

PINARCIK

2023

Turkish Journal of Chemistry

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The results of UV spectrophotometric analysis were analysed using partial least squares (PLS) and principal component regression (PCR) techniques to allow simultaneous evaluation of tramadol hydrochloride (TRA) and p-acetaminophen (PAR) in tablets. A calibration set of 16 mixtures, each containing PAR and TRA in various amounts, was created using a 2 4 -full fractional design. The absorbance data set for the calibration set were obtained between 215–280 nm (Δλ = 0.1 nm). Subsequently, the concentration and absorbance sets were used to generate PCR and PLS calibrations. The ratio spectra- first derivative method was devised as a solution to the same problem to compare the outcomes of the chemometric methods used for the same experiment. After the proposed methods were shown to be accurate in the testing of validation samples, they were used in analysing commercial tablet samples. The analytical results showed that PCR and PLS methods can be used as alternative methods to high-performance liquid chromatography (HPLC). Correlation coefficients were determined for the working concentration range of 6–36 g.mL −1 for PAR and 4–22 g.mL −1 for TRA. The limits of detection and quantification were calculated as 0.9104 μg.mL −1 and 3.0347 μg.mL −1 , respectively. The test results of the chemometric analyses and ratio spectra- first derivative method of the commercial tablet form are in agreement with the results of the one-way ANOVA with a confidence interval of 95%. This study shows that the ratio spectra- first derivative method, PCR and PLS models based on spectrophotometric measurements are very useful and straightforward techniques for the quantitative resolution of a two-component pharmaceutical preparation, requiring little sample preparation and little time for analysis.

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“…Stress degradation study by HPLC for determination of PCM in combination with other drugs 7 . Spectrophotometric methods for estimating PCM and DCF as a single drug in dosage form have also been reported [8][9][10] . No reports were found for the simultaneous estimation of the paracetamol, diclofenac and caffeine in combined dosage form by the HPTLC method.…”

Section: Introductionmentioning

confidence: 99%

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2023

IJPSR

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A new simple High-Performance Thin Layer Chromato-graphic (HPTLC) method for determining Paracetamol, Diclofenac and Caffeine in combined tablet dosage form has been developed and validated. The mobile phase selected was Ethyl acetate: Methanol: ammonia (8: 2:0.1 v/v/v) with UV detection at 263 nm. The retention factor for Paracetamol, Diclofenac, and caffeine was found to be 0.639 ± 0.018, 0.267 ± 0.026, and 0.447 ± 0.026, respectively. The method was validated with respect to linearity, accuracy, precision and robustness as per the International Conference on Harmonization (ICH) guidelines. Results were found to be linear in the concentration range of 1000-6000 ng/band for Paracetamol 200-1200 ng/band for Diclofenac and 100-600 ng/band for caffeine, respectively. The method has been successfully applied to analyze drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 98.64 ± 0.184 for paracetamol, 97.17± 1.074 for Diclofenac and 98.27± 0.86 caffeine. The method can be used for routine analysis of these drugs in combined tablet dosage forms in quality-control laboratories.

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Validation of a spectrophotometric method for the determination of paracetamol in plasma applicable for toxicological emergencies in laboratories with limited resources

Cited by 6 publications

References 11 publications

Carbon Nanotube Modified Glassy Carbon Electrodes for on‐line and off‐line in vitro Therapeutic Equivalence Assessment of Paracetamol Tablets

Carbon Nanotube Modified Glassy Carbon Electrodes for on‐line and off‐line in vitro Therapeutic Equivalence Assessment of Paracetamol Tablets

Spectrophotometric quantification of paracetamol and tramadol hydrochloride by chemometric calibration methods

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