Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma

Degreef, Maarten; Nuijs, Alexander L.N. van; Maudens, Kristof E.

doi:10.1016/j.cca.2018.06.047

Cited by 33 publications

(18 citation statements)

References 63 publications

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“…The functions of miR-7 in different endocrine organs have been reported (Moberg 1991, Kredo-Russo et al 2012, Glover et al 2015, Degreef et al 2018. However, at first, our research indicates that miR-7 acts as a regulatory factor which involves in the process of NE-mediated FSH and LH synthesis.…”

Section: Discussionmentioning

confidence: 64%

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

Zhang

et al. 2020

Journal of Endocrinology

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MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the RAF/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE’s effect on promoting FSH and LH synthesis in porcine pituitary.

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Section: Discussionmentioning

confidence: 64%

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

Zhang

et al. 2020

Journal of Endocrinology

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“…One of the steps to take into account in an analytical method is the isolation and concentration of analytes of interest from the biological samples under study; the most used procedures for extracting antidepressants are liquid-liquid extraction (LLE) [6,17], solid-phase extraction (SPE) [6,[18][19][20], and some miniaturized techniques-such as solidphase microextraction (SPME) [6,21,22], microextraction by packed sorbent (MEPS) [6,[23][24][25], and dispersive liquid-liquid microextraction (DLLME) [6,[26][27][28][29][30][31]. There are also several methods involving gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS) [26,[30][31][32][33][34][35][36][37][38] or tandem mass spectrometry (MS/MS) [27,[39][40][41][42][43], ultraviolet (UV) [22,28,[44][45][46][47][48], fluorescence [25], diode array (DAD) or photodiode array (PDA)…”

Section: Introductionmentioning

confidence: 99%

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

et al. 2021

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The increase in the consumption of antidepressants is a public health problem worldwide, as these are a class of compounds widely used in the treatment of several illnesses, such as depression and anxiety. This work aimed to develop and optimize a method for the quantification of a number of antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram, sertraline, and paroxetine) in 100 µL of oral fluid using the dried saliva spots (DSS) sampling approach and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was validated, presenting linearity within the studied range, with detection and quantification limits ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy presented acceptable coefficients of variation and relative errors, considering the criteria usually accepted in the validation of bioanalytical procedures. The method herein described is the first to be reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast alternative to conventional techniques, and capable of being routinely applied in clinical and forensic toxicology scenarios.

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“…A fast, simple LC-MS/MS method for the quantitative detection of antidepressant drugs and their metabolites in plasma for use in therapeutic drug monitoring and routine forensic analysis without the need for any time-consuming sample preparation was developed for the simultaneous quantification of 40 compounds in human plasma [ 117 ]. Samples were prepared by the addition of 1M carbonate buffer (pH 9.5) and methyl-tertiarybutyl-ether followed by vortex-mixing.…”

Section: Psychotropic Drugsmentioning

confidence: 99%

Review of Chromatographic Methods Coupled with Modern Detection Techniques Applied in the Therapeutic Drugs Monitoring (TDM)

Tuzimski

Petruczynik

2020

Molecules

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Therapeutic drug monitoring (TDM) is a tool used to integrate pharmacokinetic and pharmacodynamics knowledge to optimize and personalize various drug therapies. The optimization of drug dosing may improve treatment outcomes, reduce toxicity, and reduce the risk of developing drug resistance. To adequately implement TDM, accurate and precise analytical procedures are required. In clinical practice, blood is the most commonly used matrix for TDM; however, less invasive samples, such as dried blood spots or non-invasive saliva samples, are increasingly being used. The choice of sample preparation method, type of column packing, mobile phase composition, and detection method is important to ensure accurate drug measurement and to avoid interference from matrix effects and drug metabolites. Most of the reported procedures used liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques due to its high selectivity and sensitivity. High-performance chromatography with ultraviolet detection (HPLC-UV) methods are also used when a simpler and more cost-effective methodology is desired for clinical monitoring. The application of high-performance chromatography with fluorescence detection (HPLC-FLD) with and without derivatization processes and high-performance chromatography with electrochemical detection (HPLC-ED) techniques for the analysis of various drugs in biological samples for TDM have been described less often. Before chromatographic analysis, samples were pretreated by various procedures—most often by protein precipitation, liquid–liquid extraction, and solid-phase extraction, rarely by microextraction by packed sorbent, dispersive liquid–liquid microextraction. The aim of this article is to review the recent literature (2010–2020) regarding the use of liquid chromatography with various detection techniques for TDM.

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Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma

Cited by 33 publications

References 63 publications

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

Review of Chromatographic Methods Coupled with Modern Detection Techniques Applied in the Therapeutic Drugs Monitoring (TDM)

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