miR-7 mediates the signaling pathway of NE affecting FSH and LH synthesis in pig pituitary

Abstract

MicroRNA-7 (miR-7) is an important modulator of a plenty of gene expressions and the interrelated biological processes, highly expressed in porcine pituitary. Norepinephrine (NE), acting as an important neurotransmitter or/and a hormone secreted excessively under stress, affects the synthesis and secretion of various hormones, including pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which are the key hormones which regulate sexual maturation and reproductive functions. However, the relationship among NE, miR-7 and gonadotropin needs to be elucidated. The aim of this study was to identify whether miR-7 involved in the NE-adrenoceptor signaling pathway affects the synthesis and secretion of FSH and LH in porcine pituitary. Our results showed that the NE intracerebroventricular injection increased pituitary miR-7 level and the synthesis and secretion of FSH and LH in porcine, whereas the inhibition of either endogenous miR-7 or β-adrenergic receptors hindered the rise of FSH and LH synthesis induced by NE in cultured primary porcine anterior pituitary cells. Further, we identified the molecular type of β-adrenergic receptors and the signaling pathway in porcine pituitary, and we found that NE played its roles relying on adrenoceptor beta 2 (β2AR) and the RAF/MEK/ERK1/2 signaling pathway. The phosphorylation of ERK1/2 upregulated miR-7 level which subsequently enhanced FSH and LH synthesis by targeting to Golgi glycoprotein 1 (GLG1). These suggest that miR-7 mediates NE’s effect on promoting FSH and LH synthesis in porcine pituitary.

The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway

“…It has been reported that miRNAs are abundant in the central nervous system [44]. miRNAs have been shown to regulate FSHβ expression and further affect FSH secretion, such as miR-361-3p [45] and miRNA-7 [46]. In this study, we found that a known miRNA may be involved in the regulation of pituitary function.…”

MicroRNA-7a2 Contributes to Estrogen Synthesis and Is Modulated by FSH via the JNK Signaling Pathway in Ovarian Granulosa Cells

“…The function of miR-7a2 in various endocrine organs has been reported in several studies [ 18 , 20 , 22 , 23 ]. Previous studies demonstrated that miR-7a2 affects the gonad development and reproductive function of mice by modulating the synthesis and secretion of the FSH and LH.…”

“…The vector containing Not I and Xho I sites and the miR-7a binding sites of G lg1 and 5′-GCUUGU-3′ was used for the reporter assay. The 293FT cells were cultured in 10% FBS-DMEM/F12 in 24-well plates and transfected using a Lipofectamine 2000 agent (Invitrogen Life Technologies, Gaithersburg, MD, USA) with a mixture containing the dual-luciferase reporter plasmid, and negative control of miR-7a mimics (nc-mi) or miR-7a mimics (miR-7a-mi) for 24 h, as described previously [ 22 , 23 ]. The luciferase activity was analyzed using the dual-luciferase reporter assay kit (E1910; Promega).…”

Механізми Впливу Міді На Відтворну Здатність Самців

“…Норадреналін, діючи як важливий нейротрансмітер, впливає на синтез та секрецію різних гормонів цієї системи, зокрема фолікулостимулюючого гормону (ФСГ) та лютеїнізуючого гормону (ЛГ), котрі які є ключовими у становленні та регулюванні статевої функції. Згідно з даними X. Li, встановлено, що посилений рівень норадреналіну в організмі кнурців (в період становлення статевої зрілості) сприяє посиленню синтезу ФСГ та ЛГ у гіпофізі через β2AR [24]. У сім'яниках, рецептори ФСГ знаходяться на мембрані клітин Сертолі, а рецептори ЛГ -на клітинах Лейдіга, тому вони регулюють синтез тестостерону та забезпечують нормальний сперматогенез [5].…”