Validation of a reversed-phase LC method for quantitative analysis of intravenous admixtures of ciprofloxacin and metronidazole

Vega, Elena María; Dabbene, Viviana G.; Nassetta, M.; Solá, Nancy Haydée

doi:10.1016/s0731-7085(99)00218-6

Cited by 26 publications

(14 citation statements)

References 5 publications

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“…Metronidazole 2-methyl-5-nitroimidazole-1-ethanol [1] was determined individually by short-wave length NIR spectroscopy [2], voltammetry [3][4][5], NMR spectrometry [6], gas chromatography [7] and HPLC methods either alone [8][9][10] or in the presence of its metabolites [11,12] or in the presence of its degradation product [13], in addition to its mixture with other drugs [14,15]. First derivative spectrophotometry was used for the determination of metronidazole in mixture with ciprofloxacin [16].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation

Ghobashy

Abo-Talib

2010

Journal of Advanced Research

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Ratio subtraction and isosbestic point methods are two innovative spectrophotometric methods for determining the concentrations of metronidazole (I) and diloxanide furoate (II) in a mixture. Metronidazole was determined by direct spectrophotometric method at k max 314.0 nm in the presence of diloxanide furoate in the range of 4-24 lg ml À1 with a mean recovery percentage of 99.83 ± 1.41. Two spectrophotometric methods were developed for the spectral resolution of diloxanide furoate when present in mixture with metronidazole without preliminary separation. The first method depends on measuring the absorbance at the isosbestic point at 277.2 nm in the range of 5-30 lg ml À1 with a mean recovery percentage of 99.96 ± 1.47 for diloxanide furoate. The second method is the ratio subtraction spectroscopic method for spectral isolation of diloxanide furoate present in the mixture which can be measured at 251.2 nm in the range of 5-30 lg ml À1 with a mean recovery percentage of 99.73 ± 1.33 for diloxanide furoate determination. The suggested procedures were validated using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical preparations. The methods retained their accuracy and precision when the standard addition technique was applied. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the reported method.

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Section: Introductionmentioning

confidence: 99%

Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation

Ghobashy

Abo-Talib

2010

Journal of Advanced Research

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“…A previous work by Vega et al [30] described a reversed-phase isocratic LC method for the determination of intravenous admixtures of commercial products containing CIP and MET. The mobile phase was composed of a mixture of 0.1 M monobasic sodium phosphate solution (pH 3.3) and acetonitrile (80:20, v/v) on a LiChrosorb (Merck) column, 25-cm length, 4 mm i.d., and particle size 10 mm and UV detection at 278 nm was applied.…”

Section: Resultsmentioning

confidence: 99%

“…MET has been analyzed by spectrophotometry [19][20][21][22] and HPLC [23][24][25][26][27][28]. MET in intravenous admixture with CIP has been determined by first-derivative spectrophotometry [29] and LC [30]. However, no previous method has been adopted for the simultaneous determination of CIP and MET in tablets.…”

Section: Introductionmentioning

confidence: 99%

Reversed-Phase Ion-Pair HPLC and TLC-Densitometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Elkady

Mahrouse

2011

Chromatographia

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Two methods are described for the simultaneous determination of ciprofloxacin HCl (CIP) and metronidazole (MET) in binary mixture. The first method was based on the ion-pair liquid chromatographic separation of the two drugs on reversed-phase, lBondapak C 18 column (250 mm 9 4.6 mm, 10 lm) Waters. The mobile phase consisted of monobasic potassium phosphate (50 mM, pH 3, adjusted with phosphoric acid) and acetonitrile (65:35, v/v) containing sodium octane sulphonate (50 mM). Flow rate of 1.2 mL min -1 was applied. Quantitation was achieved with UV detection at 280 nm. Linearity, accuracy and precision were found to be acceptable over the concentration range of 3-180 and 15-180 lg mL -1 for CIP and MET, respectively. The second method was based on the TLC separation of the two drugs followed by densitometric measurements of their bands at 280 nm. The separation was carried out on silica gel 60 F 254 plates, using acetonitrile, ammonia, methanol, methylene chloride and hexane (1.3:1.1:2.0:3.0:1.0, v/v) as developing system. The linear regression analysis data were used for the regression line in the range of 1.5-10 lg band -1 for CIP and MET. The two proposed methods were successfully applied to the determination of both drugs in laboratory prepared mixtures and in commercial tablets. The optimized methods proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparation.

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“…[27][28][29][30][31][32] MET in intravenous admixture with CIP was determined by first-derivative spectrophotometry 33) and LC. 34) Literature reports only one article for the simultaneous determination of CIP and MET in tablet form by the same authors of the present work. In this article, a reversed phase ion-pair HPLC and TLC densitometry have been adopted for the simultaneous determination of both drugs in tablets.…”

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confidence: 88%

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Mahrouse

Elkady

2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Regular ArticleCiprofloxacin (CIP, Fig. 1a) [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] belongs to the second generation of fluoroquinolone antibacterial agents. These quinolones are effective against Grampositive and Gram-negative bacteria through inhibiting bacterial DNA gyrase (topoisomerase), the enzyme responsible for maintaining the superhelical twists in the bacterial DNA, leading to inhibition of bacterial DNA synthesis.1) Similar to other fluoroquinolones, CIP has shown clinical efficacy in the treatment of a wide range of systemic infections such as urinary tract, respiratory, gastrointestinal and cutaneous infections. Because of its low toxicity, very wide spectrum of antibacterial effect and low ability to cause bacterial resistance, CIP has been widely used in clinical practice.2) Metronidazole (MET, Fig. 1b) is used as an antiprotozoal, antiamebic and antibacterial drug.3) It is chemically designated as 2-methyl-5-nitroimidazole-1-ethanol. Since CIP has a reduced activity against anaerobic pathogens, CIP and MET are coformulated together in pharmaceutical dosage forms for the treatment of mixed aerobic/anaerobic infections. 4)A survey of literature has revealed several analytical methods for the determination of CIP in pharmaceutical dosage form and biological fluids, including spectrophotometry, [5][6][7][8] spectrofluorimetry, 9-11) HPLC, 12-16) capillary electrophoresis 17,18) and HPTLC. 19) CIP in admixtures with MET and ampicillin has been determined by NMR. 20) Recently, HPLC methods either with fluorescence detection or coupled with mass spectrometry (LC/MS) for determination of ciprofloxacin in human plasma 21,22) have also been published. MET has been determined by several methods involving spectrophotometry [23][24][25][26] and HPLC. [27][28][29][30][31][32] MET in intravenous admixture with CIP was determined by first-derivative spectrophotometry 33) and LC. 34) Literature reports only one article for the simultaneous determination of CIP and MET in tablet form by the same authors of the present work. In this article, a reversed phase ion-pair HPLC and TLC densitometry have been adopted for the simultaneous determination of both drugs in tablets.35) However, due to lack of such equipments in many resources-limited countries and the high costs of HPLC grade solvents and columns, alternative methods are needed to facilitate and increase the speed of analysis, with relatively few costs. To the best of the authors' knowledge, no previous article concerning simultaneous spectrophotometric determination of the two drugs was published. Spectrophotometry continues to be very popular, because of its simplicity, versatility and low cost. Therefore, the aim of the present work was to develop and validate different UV spectrophotometric and chemometric techniques for the simultaneous determination of CIP and MET in tablets without previous separation. Both derivative and derivative ratio spectrophotometry are useful technique for resolving two overlapping...

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Validation of a reversed-phase LC method for quantitative analysis of intravenous admixtures of ciprofloxacin and metronidazole

Cited by 26 publications

References 5 publications

Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation

Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation

Reversed-Phase Ion-Pair HPLC and TLC-Densitometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

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