Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation

Abstract

Ratio subtraction and isosbestic point methods are two innovative spectrophotometric methods for determining the concentrations of metronidazole (I) and diloxanide furoate (II) in a mixture. Metronidazole was determined by direct spectrophotometric method at k max 314.0 nm in the presence of diloxanide furoate in the range of 4-24 lg ml À1 with a mean recovery percentage of 99.83 ± 1.41. Two spectrophotometric methods were developed for the spectral resolution of diloxanide furoate when present in mixture with metronidazole without preliminary separation. The first method depends on measuring the absorbance at the isosbestic point at 277.2 nm in the range of 5-30 lg ml À1 with a mean recovery percentage of 99.96 ± 1.47 for diloxanide furoate. The second method is the ratio subtraction spectroscopic method for spectral isolation of diloxanide furoate present in the mixture which can be measured at 251.2 nm in the range of 5-30 lg ml À1 with a mean recovery percentage of 99.73 ± 1.33 for diloxanide furoate determination. The suggested procedures were validated using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical preparations. The methods retained their accuracy and precision when the standard addition technique was applied. The results obtained by applying the proposed methods were statistically analyzed and compared with those obtained by the reported method.

Development of gradient HPLC-DAD method for assay of ternary mixture containing amebicide and analgesic drugs

“…The two methods gave more or less concordant results with respect to their precision (F-test) and accuracy (t-test) where the calculated values did not exceed the theoretical values for either one of the three drugs. On the other hand, the proposed HPLC-DAD method can be considered superior to the previously reported method [7] because it is more sensitive and selective. The proposed method provides lower values of LOD and LOQ, for the three drugs than in reported method.…”

“…The results of analysis of the commercial tablets and the recovery study (standard addition method) of drugs (Table VI) suggested that there is no interference from any excipients, which are normally present in tablets. In addition, the results obtained using the proposed HPLC method were statistically compared with those obtained with the reported TLC method [7]. The two methods gave more or less concordant results with respect to their precision (F-test) and accuracy (t-test) where the calculated values did not exceed the theoretical values for either one of the three drugs.…”

“…Moreover, to check the validity of the proposed methods, the standard addition method was applied by adding of DIL, MBV, and MET to the previously analyzed tablets. The recovery b TLC method [7]. c Mean ± standard deviation of five determinations.…”

“…Dimetrol®, a described commercial product, launched by EVA PHARMA for Pharmaceuticals and Medical Appliances, contains a combination of amoebicide and antispasmodic drugs. The literature survey reveals only one published paper for simultaneous determination of DIL, MEV, and MET [7]. This paper described derivative ratio spectrophotometric and TLC-densitometric methods for assay of ternary mixture of these drugs in pharmaceutical preparation.…”

Rapid Determination of Quinoline and 2-Hydroxyquinoline in Quinoline Biodegradation Process by Tri-Wavelength UV/Vis Spectroscopy

“…Although the overlapped spectra of Q and HQ make it difficult to quantify these species based on the spectral measurement at a single wavelength, we believe that the problem can be resolved by using novel data acquisition approaches, such as dual-wavelength or tri-wavelength techniques. [13][14][15] In this work, we developed a novel, tri-wavelength spectrophotometric method for the determination of Q and HQ in sample solutions obtained from biochemical conversion processes. The data obtained by the present method were compared with those obtained by HPLC.…”