Validation of a noninvasive diagnostic tool to verify neuter status in dogs: The urinary FSH to creatinine ratio

Albers-Wolthers, C.H.J.; Gier, J. De; Oei, Christine; Schaefers-Okkens, A.C.; Kooistra, Hans S.

doi:10.1016/j.theriogenology.2016.04.081

Cited by 1 publication

(2 citation statements)

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“…Extraction and potency of hormones with biological sources have encountered some clinical challenges including individual variability, pulsatile secretion and immune reactions [89]. Individual variation in gonadotropin secretary pattern have been reported in PMSG in mares [5], human menopausal gonadotropin [14] and FSH in dogs [1]. It is important to point out that amplitude, frequency and total concentration of hormones varies among individuals in each species and is affected by their age and body conditions.…”

Section: Discussionmentioning

confidence: 99%

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Induction of superovulation in mature mice and rats using serum of spayed female dogs

et al. 2018

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The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (±SEM) number of CLs between the PMSG group and the mice that received serum of spayed dog (10.4±1.3 vs 9.2±1.0). Mean (±SEM) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH (15.1±1.9 vs 23.6±3.1 and 23.1±2.9, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

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Section: Discussionmentioning

confidence: 99%

“…After spaying, there would be no estrogen and progesterone feedback control on pituitary releasing hormones. Mean serum FSH concentration in ovario-hysterectomized bitches is higher than those sexually intact bitches [112]. Further, the concentration of FSH is higher in ovariectomized dogs (81.7 µg/L) than those of the anestrus dogs (5.6 µg/L) [2].…”

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confidence: 99%