Induction of superovulation in mature mice and rats using serum of spayed female dogs

Abstract

The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (±SEM) number of CLs between the PMSG group and the mice that received serum of spayed dog (10.4±1.3 vs 9.2±1.0). Mean (±SEM) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH (15.1±1.9 vs 23.6±3.1 and 23.1±2.9, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

“…GO analysis was performed using the Panther database (http://www.pantherdb.org). GO terms "biological process" were the most represented (>4 genes) among R-eCG-treated ovarian tissue, including "signal transduction (16 genes)," "developmental processes (14)," "protein metabolism and modi cation (9)," "cell culture and motility (5)," and "nucleoside, nucleotide and nucleic acid metabolism (4)." In category "molecular function," these genes were classi ed into 18 subcategories through GO analysis, with the largest number of genes represented in "protease (6 genes)," "signaling molecule (5)," "oxidoreductase (5)," "nucleic acid binding (5)," and "hydrolase (5)."…”

Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

“…GO analysis was performed using the Panther database (http://www.pantherdb.org). GO terms "biological process" were the most represented (>4 genes) among R-eCG-treated ovarian tissue, including "signal transduction (16 genes)," "developmental processes (14)," "protein metabolism and modi cation (9)," "cell culture and motility (5)," and "nucleoside, nucleotide and nucleic acid metabolism (4)." In category "molecular function," these genes were classi ed into 18 subcategories through GO analysis, with the largest number of genes represented in "protease (6 genes)," "signaling molecule (5),"…”

Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

“…Owing to its long half-life in blood, a single dose of eCG, as opposed to multiple doses, is adequate to stimulate ovarian gene expression [13]. Furthermore, eCG and human CG (hCG) together stimulate ovulation in rats and mice [14,15]. Moreover, eCG administration in cows is reportedly associated with an increase in their ovulation rate [16], particularly in early postpartum calves [17,18].…”