Validation of a methodology by LC-MS/MS for the determination of triazine, triazole and organophosphate pesticide residues in biopurification systems

Masís-Mora, Mario; Beita-Sandí, Wilson; Rodríguez-Yáñez, Javier E.; Rodríguez-Rodríguez, Carlos E.

doi:10.1016/j.jchromb.2020.122296

Cited by 19 publications

(5 citation statements)

References 67 publications

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“…By adding 6 μL of K 2 CO 3 (0.1 M), the pH of the solution was adjusted. Subsequently, different volumes (6,8,10,12,14,16, and 18 μL) of the prometryn antibody (1 mg/mL) were added to each tube. The tubes were then incubated at room temperature for 10 min, followed by the addition of 20 μL of a 10% BSA solution for blocking and further incubation for 10 min.…”

Section: Preparation Of Colloidal Goldmentioning

confidence: 99%

“…Additionally, the maximum allowable residue limit for prometryn in rice, corn, garlic, and lotus roots is set at 0.05 mg/kg. Currently, instrumental methods such as high-performance liquid chromatography (HPLC), − gas chromatography–tandem mass spectrometry (GC–MS/MS), , and liquid chromatography–tandem mass spectrometry (LC–MS/MS) − are commonly employed to detect prometryn in various matrices. Although these methods offer sensitivity and accuracy, they require expensive equipment and specialized personnel, and their relatively long detection times make them unsuitable for rapid screening.…”

Section: Introductionmentioning

confidence: 99%

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Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Gao,

Abd El-Aty,

et al. 2024

ACS Agric. Sci. Technol.

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This study aimed to establish two methods, namely, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic test strips, for detecting prometryn residues in various agricultural samples, such as corn, pumpkin, lotus root, rice, and brown rice. Monoclonal antibodies specific for prometryn were utilized in both methods. The ic-ELISA method demonstrated a maximum half-inhibition concentration (IC 50 ) of 2.4 ng/mL and a lower limit of detection of 0.5 ng/mL. When used in conjunction with a card reader, the colloidal gold immunochromatographic test strip exhibited an IC 50 of 18.6 ng/mL for prometryn detection. The recovery rates of both methods were found to be consistent with those of liquid chromatography tandem mass spectrometry. This consistency indicated the suitability of both methods for detecting prometryn in five different agricultural samples, suggesting that this is a convenient and practical approach for screening prometryn in agricultural products.

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Section: Preparation Of Colloidal Goldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Gao,

Abd El-Aty,

et al. 2024

ACS Agric. Sci. Technol.

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“…Instrumental analytical methods, such as gas chromatographytandem mass spectrometry [5] and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) [6][7][8], are the standard methods used for the detection of triazine residues. Although these methods are highly accurate, sensitive and reliable, some disadvantages such as the need for large amounts of organic solvents, complicated sample pre-processing steps, bulky instrumentation, long detection time, high cost and the need for professional operators, make them not suitable for onsite detection.…”

Section: Introductionmentioning

confidence: 99%

Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

Guo

Zhao

et al. 2022

Nano Res.

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Triazine herbicides have been widely used in agriculture, but their residues can harm the environment and human health. To help monitor these, we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples (including ametryn, cyprazine, atraton, prometon, prometryn, atrazine, propazine, terbuthylazine, simetryn, trietazine, secbumeton, simazine, desmetryn, terbumeton and simetone). Based on our optimization procedure, the visual limit of detection (vLOD) for these triazines was found to be 2–10 ng/mL in assay buffer, and 0.02−0.1 mg/kg in grain samples. Four different grain matrices including corn, brown rice, wheat, and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this method. This test is simple, convenient, rapid, and low-cost, and could be an effective tool for primary screening of triazine residues in grain samples. Electronic Supplementary Material Supplementary material (UV–vis spectra of 15 nm-GNP; K2CO3 usage; cross reactivity; strip images for spiked rice, wheat and sorghum samples, UPLC-MS/MS parameters; gray values for strip optimization) is available in the online version of this article at 10.1007/s12274-022-4164-2.

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“…At present, chlorpyrifos residues are mainly detected by instrumental methods such as gas chromatography (GC) [ 4 , 5 ], gas chromatography-mass spectrometry (GC-MS) [ 6 , 7 , 8 , 9 ], high performance liquid chromatography (HPLC) [ 10 ], and liquid chromatography-mass spectrometry (LC-MS) [ 7 , 11 ]. Although these detection methods have the advantages of high accuracy and sensitivity, they require expensive instruments, professional technicians, tedious sample pretreatment processes, and large amounts of organic solvents, which limit their use for the rapid detection of chlorpyrifos residues in the field.…”

Section: Introductionmentioning

confidence: 99%

A Simple Aptamer SERS Sensor Based on Mesoporous Silica for the Detection of Chlorpyrifos

Dong

Shi

et al. 2022

Foods

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Chlorpyrifos is an organophosphorus insecticide, which can be used to control a variety of chewing and piercing mouthparts pests in agricultural production. It can destroy the normal nerve impulse conduction by inhibiting the activity of acetylcholinesterase or cholinesterase in the nerves, causing a series of poisoning symptoms. In order to achieve the quantitative analysis of chlorpyrifos residues in agricultural products, an aptamer-controlled signal molecule release method was developed in this study. The signal molecule 4-ATP of surface-enhanced Raman spectroscopy (SERS) was loaded into aminated mesoporous silica nanoparticles (MSNs-NH2) prepared by the one pot method, and then coated with an aptamer of chlorpyrifos through electrostatic interaction. The specific binding of the aptamer and chlorpyrifos led to the release of 4-ATP, and the amount of 4-ATP released was positively correlated with the amount of chlorpyrifos. Finally, the standard curve of chlorpyrifos quantitative detection based on SERS was established. Meanwhile, Ag-carrying mesoporous silica (Ag@MSNs) was prepared as the reinforcement substrate for SERS detection. The results showed that there was a good linear correlation between the Raman intensity and the concentration of chlorpyrifos at 25–250 ng/mL, and the limit of detection (LOD) was 19.87 ng/mL. The recoveries of chlorpyrifos in the apple and tomato samples were 90.08–102.2%, with RSD < 3.32%. This method has high sensitivity, specificity, reproducibility and stability, and can be used for the quantitative detection of chlorpyrifos in the environment and agricultural products.

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Validation of a methodology by LC-MS/MS for the determination of triazine, triazole and organophosphate pesticide residues in biopurification systems

Cited by 19 publications

References 67 publications

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

A Simple Aptamer SERS Sensor Based on Mesoporous Silica for the Detection of Chlorpyrifos

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