Validation of a high-performance thin-layer chromatography/densitometry method for the quantitative determination of glucosamine in a herbal dietary supplement

Esters, Virginie; Angenot, Luc; Brandt, Viviane; Frédérich, Michel; Tits, Monique; Nerum, Christophe Van; Wauters, Jean-Noël; Hubert, Philippe

doi:10.1016/j.chroma.2006.01.035

Cited by 35 publications

(17 citation statements)

References 17 publications

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“…Besides HPLC technique, capillary electrophoresis (CE) with UV detection (214 nm) after the derivatisation of GA with anthranilic acid (Qi et al 2006) or with conductometric detection (Jáč et al 2008) without derivatisation have been used. Other described techniques suitable for the analysis of GA involve spectrophotometry after derivatisation with ninhydrin (Wu et al 2005), and high performance thin-layer chromatography (Esters et al 2006).…”

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confidence: 99%

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Václavíková¹,

Kvasnička²

2013

Czech J. Food Sci.

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Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech J. Food Sci., 31: 55-65.Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many of the marketed products. We present here a capillary isotachophoretic method for the determination of glucosamine and chondroitin sulfate in dietary supplements. Cationic analysis of glucosamine was performed with a leading electrolyte consisting of 10mM NH 4 OH + 20mM acetic acid. As the leading electrolyte for anionic analysis of chondroitin sulphate, a mixture of 5mM HCl + 10mM glycylglycine + 0.05% of 2-hydroxyethylcellulose was used. The solution of 10mM citric acid served as the terminating electrolyte for both glucosamine and chondroitin sulfate analyses. The analytes were detected by conductivity and UV detectors. The characteristics of the method,, i.e., linearity, accuracy, repeatability, and quantitation limit, were evaluated. On a set of 35 samples of marketed dietary supplements did we prove that the capillary isotachophoresis is a suitable method for the routine analysis of glucosamine and chondroitin sulfate.

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confidence: 99%

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Václavíková¹,

Kvasnička²

2013

Czech J. Food Sci.

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“…The authors presented an example where the best regression model during the prevalidation was not optimal compared to the best model obtained with all validation data. A separate study reported a pre-validation/validation plan for a high performance TLC assay of glucosamine in a dietary supplement [5]. In this case, the investigators used pre-validation calibration data to evaluate six regression models and selected the optimal model based on accuracy profiles.…”

Section: Introductionmentioning

confidence: 98%

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Wood

Peoples

2012

Chromatographia

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HPLC methods for pharmaceutical analysis evolve from method development to commercial product testing. The majority of development and validation takes place in an R&D setting, followed by transfer to commercial sites that execute test methods on a routine basis. There is a growing need to increase confidence and probability that developed methods will be successful both during validation and long-term use with products, particularly when introducing new or unfamiliar technologies. An HPLC method pre-validation and performance prediction plan is presented using model pharmaceutical ingredients in prototype liquid formulations. The pre-validation includes limited linearity, repeatability in sample matrix, precision, carry-over, and selectivity. The performance prediction involves injecting large numbers of worst-case samples and monitoring five critical parameters: retention time, tailing factor, resolution, efficiency, and system pressure. Parameter results are assessed with respect to precision and/or system performance. In a proof of principle model, the performance prediction data demonstrate that system suitability parameters remain constant during injections of prepared samples; however, the system pressure increases over time. These underlying data indicate a potential trend prior to method validation. As a result, sample preparation and HPLC condition modifications are evaluated using pressure plots as a key performance metric.

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“…The total error approach has proven itself to be efficacious in allowing evaluation of a number of methods such as UV [19], NIR [19], HPTLC [20], HPLC [21], GC [22], and LC-MS/MS [23] methods of quantification. We propose to illustrate its usefulness with a LC-MS/MS method intended to quantify residual concentrations of monensin not only in plasma, but also in complex matrices such as muscle, fat and liver.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

Chéneau

Henri

Pirotais

et al. 2007

Journal of Chromatography B

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Validation of a high-performance thin-layer chromatography/densitometry method for the quantitative determination of glucosamine in a herbal dietary supplement

Cited by 35 publications

References 17 publications

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

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