Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Abstract

Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech J. Food Sci., 31: 55-65.Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many of the marketed products. We present here a capillary isotachophoretic method for the determination of glucosamine and chondroitin sulfate in dietary supplements. Cationic analysis of glucosamine was performed with a leading electrolyte consisting of 10mM NH 4 OH + 20mM acetic acid. As the leading electrolyte for anionic analysis of chondroitin sulphate, a mixture of 5mM HCl + 10mM glycylglycine + 0.05% of 2-hydroxyethylcellulose was used. The solution of 10mM citric acid served as the terminating electrolyte for both glucosamine and chondroitin sulfate analyses. The analytes were detected by conductivity and UV detectors. The characteristics of the method,, i.e., linearity, accuracy, repeatability, and quantitation limit, were evaluated. On a set of 35 samples of marketed dietary supplements did we prove that the capillary isotachophoresis is a suitable method for the routine analysis of glucosamine and chondroitin sulfate.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…Detection limits for the method ranged from 0.030 to 0.097 mg/L with RSDs values lower than 4.5%. Capillary ITP has also been applied for the analysis of glucosamine []. The validation of the method demonstrated recovery values of 98.8%, LOD of 2.4 mg/L, and a good precision (RSD = 1.82%).…”

Determination of glucosamine and monitoring of its mutarotation by hydrophilic interaction liquid chromatography with evaporative light scattering detector

“…a blue molybdosilicate anion (Katano, Takakuwa, Hayakawa, & Kimoto, 2016), or by an enzymatic method (Widyaningsih et al, 2017). Several papers have utilized capillary electrophoresis (Qi, Zhang, Zuo, & Chen, 2006;Chaisuwan et al, 2011;Václavíková & Kvasnička, 2013); however, most of the articles concern glucosamine determination by chromatography.…”

Quality control of chondroitin sulphate used in dietary supplements

“…The recovery of CS was 92.4% and the method was applicable for the qualitative and quantitative analysis of CS in commercial cosmetic products. Recently, we reported on the application of cITP methods for the determination of CS and glucosamine quantities in dietary supplements (Václavíková & Kvasnička 2013). In this study we present the modified cITP method for the evaluation of quality of CS raw materials used for dietary supplements production.…”