Validation of a dried blood spot method for therapeutic drug monitoring of citalopram, mirtazapine and risperidone and its active metabolite 9-hydroxyrisperidone using HPLC–MS

Weber, Johanna; Oberfeld, Stefanie; Bonse, Andrea; Telger, Klaus; Lingg, Rainer; Hempel, Georg

doi:10.1016/j.jpba.2017.02.061

Cited by 29 publications

(13 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The self-sampling and almost painless sample collection is a big advantage of DBS, even if the samples can be possibly contaminated, if the person preparing them and dosing the drug is the same. The results obtained by the DBS method are comparable with plasma, so DBS sampling is an excellent alternative (80)(81)(82). DBS can be used also in forensic toxicology, for example in screening the abuse substances, like cannabinoids (83), cocaine (84), opiates (85) and so on (86).…”

Section: Applications Of the Dbs Samplesmentioning

confidence: 95%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

View full text Add to dashboard Cite

The minimization is currently expanding in all fi elds of human life. The smaller size of analytical instruments and their higher sensitivity enables an analysis of small amounts of a biological material with a very low consumption of chemicals, what is economically and also environmentally benefi cial. A patient friendly and minimal invasive sample collection is therefore more than required. The dried blood spot (DBS) sampling standardly used in the newborn screening (NS) may be an option. The sample collection is simple, non-invasive, does not require trained medical personnel assistance and also storing and transportation of these samples is much easier in comparison with the whole blood samples. The DBS sampling method is used in the therapeutic drug monitoring or in the diagnosis of infectious diseases and its usage is still spreading. In some cases, it is still only a research object, but it shows a big potential for a future use. It could completely replace the whole venous blood collection in some cases, for example in the metabolic screening of diabetic patients or monitoring of treatment response, and it can overall simplify the sample collection and the transportation process (Tab. 1, Ref. 152).

show abstract

Section: Applications Of the Dbs Samplesmentioning

confidence: 95%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

View full text Add to dashboard Cite

show abstract

“…Dried blood spots (DBS) represent a bio-sampling method that has been developed recently for therapeutic drug monitoring [ 9 ]. The main benefits of DBS include a potential for long storage periods at room temperature, convenient transport in envelopes, and minimally-invasive blood sampling without direct sample processing.…”

Section: Introductionmentioning

confidence: 99%

Quantification of 6-Mercaptopurine and Its Metabolites in Patients with Acute Lympoblastic Leukemia Using Dried Blood Spots and UPLC-MS/MS

Supandi

Harahap²,

Harmita³

et al. 2018

Sci. Pharm.

View full text Add to dashboard Cite

This research aimed to quantitatively bioanalyze 6-mercaptopurine (6-MP), 6-methylmercaptopurine (6-MMP), and 6-thioguanosine-5′-monophosphate (6-TGMP) in dried blood spots (DBS) prepared from a small volume of acute lymphoblastic leukemia (ALL) patients. Analytes on the DBS card were extracted using 90% methanol with 5-fluorouracil (5-FU) as an internal standard. Analytical separation was performed on a Waters Acquity® UPLC BEH AMIDA column of 1.7 μm (2.1 × 100 mm) with a mobile phase mixture of 0.2% formic acid in water and 0.1% formic acid in acetonitrile-methanol, with gradient elution and a flow rate of 0.2 mL/min. Mass detection of 6-MP, 6-MMP, 6-TGMP, and 5-FU showed m/z values of 153.09 > 119.09, 167.17 > 126.03, 380.16 > 168.00, and 129.09 > 42.05, respectively. This DBS method had a run time of 5 min and yielded a linear calibration curve over a range of 25.5–1020 ng/mL for 6-MP, 6-MMP, and 6-TGMP. Analyte analysis in 22 of 24 ALL patients showed that the measured value of 6-TGMP as an active metabolite was in the range of 29–429 pmol/8 × 108 erythrocytes. Five of 22 patients had concentrations in a therapeutic range, which indicates that the treatment is effective, while 17 of 24 patients had concentrations below the therapeutic range, which indicates that a treatment dose adjustment is needed. The measured value of 6-MMP, an inactive metabolite, was in the range of 28–499 pmol/8 × 108 erythrocytes, which includes concentrations below the hepatotoxic range. The method employed here can thus be effectively utilized to support therapeutic drug monitoring.

show abstract

“…In most cases, it is recommended to monitor both parent and metabolite compounds, as both can contribute to the therapeutic and toxic effects. Thus, TDM enables achieving a rapid response to treatment and therefore increases the safety of antipsychotic treatment [ 111 ]. Often, metabolites of psychotropic drugs are active compounds and are simultaneously determined with parent drugs in biological samples.…”

Section: Psychotropic Drugsmentioning

confidence: 99%

“…The minimally invasive dried blood spots sampling technique is recognized as a suitable alternative for conventional sampling methods, as TDM interventions should be applied in the most cost-effective, rational, and clinically useful manner. A method for the simultaneous quantification of four antipsychotics and antidepressants: citalopram, mirtazapine, risperidone, and its active metabolite 9-hydroxyrisperidone in dried blood spot using HPLC-MS for therapeutic drug monitoring was described [ 111 ]. For sample preparation, the analytes from dried blood spots were extracted by methanol using an ultrasonic bath.…”

Section: Psychotropic Drugsmentioning

confidence: 99%

Review of Chromatographic Methods Coupled with Modern Detection Techniques Applied in the Therapeutic Drugs Monitoring (TDM)

Tuzimski

Petruczynik

2020

Molecules

View full text Add to dashboard Cite

Therapeutic drug monitoring (TDM) is a tool used to integrate pharmacokinetic and pharmacodynamics knowledge to optimize and personalize various drug therapies. The optimization of drug dosing may improve treatment outcomes, reduce toxicity, and reduce the risk of developing drug resistance. To adequately implement TDM, accurate and precise analytical procedures are required. In clinical practice, blood is the most commonly used matrix for TDM; however, less invasive samples, such as dried blood spots or non-invasive saliva samples, are increasingly being used. The choice of sample preparation method, type of column packing, mobile phase composition, and detection method is important to ensure accurate drug measurement and to avoid interference from matrix effects and drug metabolites. Most of the reported procedures used liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques due to its high selectivity and sensitivity. High-performance chromatography with ultraviolet detection (HPLC-UV) methods are also used when a simpler and more cost-effective methodology is desired for clinical monitoring. The application of high-performance chromatography with fluorescence detection (HPLC-FLD) with and without derivatization processes and high-performance chromatography with electrochemical detection (HPLC-ED) techniques for the analysis of various drugs in biological samples for TDM have been described less often. Before chromatographic analysis, samples were pretreated by various procedures—most often by protein precipitation, liquid–liquid extraction, and solid-phase extraction, rarely by microextraction by packed sorbent, dispersive liquid–liquid microextraction. The aim of this article is to review the recent literature (2010–2020) regarding the use of liquid chromatography with various detection techniques for TDM.

show abstract

Validation of a dried blood spot method for therapeutic drug monitoring of citalopram, mirtazapine and risperidone and its active metabolite 9-hydroxyrisperidone using HPLC–MS

Cited by 29 publications

References 15 publications

The dried blood spot sampling method in the laboratory medicine

The dried blood spot sampling method in the laboratory medicine

Quantification of 6-Mercaptopurine and Its Metabolites in Patients with Acute Lympoblastic Leukemia Using Dried Blood Spots and UPLC-MS/MS

Review of Chromatographic Methods Coupled with Modern Detection Techniques Applied in the Therapeutic Drugs Monitoring (TDM)

Contact Info

Product

Resources

About