Validation of a DNA IQ™-based extraction method for TECAN robotic liquid handling workstations for processing casework

Frégeau, Chantal J.; Lett, C. Marc; Fourney, Ron M.

doi:10.1016/j.fsigen.2009.11.001

Cited by 46 publications

(15 citation statements)

References 21 publications

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“…Poorer results obtained through the use of the Chelex ® 100 method could be due to the absence of a purifi cation step incorporated into the EZ1 ® protocol, especially considering that the Chelex resin itself is a potential PCR inhibitor. Other infl uencing factors might be the alkaline environment in which the Chelex procedure takes place, as well as the elevated temperature required for denaturation of DNA and the inactivation of proteinase K. It might be of future interest for trace DNA processing to contrast both methods against other automated DNA extraction techniques, since recent studies have described losses during sample collection and DNA extraction of up to 75% when manual extraction methods were used [12].…”

Section: Discussionmentioning

confidence: 99%

Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

et al. 2016

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Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex ® 100 method, Qiagen EZ1 ® DNA Investigator Kit), as well as to examine the morphological differences of hair fi bres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary fi ndings suggest that the use of EZ1 ® DNA Investigator Kit yields donor DNA profi les of higher quality. The results of different sample collection methods have shown intraspecifi c variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite signifi cant morphological differences between their coat hairs.

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Section: Discussionmentioning

confidence: 99%

Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

et al. 2016

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“…Actually, in cases of degraded samples or when only minute amounts of DNA are available, the use of silicacoated magnetic beads to capture the molecules from the rest of the lysed cells is recommended. These extraction procedures are also performed in some laboratories by robotic systems (Greenspoon et al, 2004;Frégeau et al, 2010). The loss of DNA during the extraction step could be linked to the substrate sustaining the sample.…”

Section: Dna Extractionmentioning

confidence: 99%

Avoiding Errors and Pitfalls in Evidence Sampling for Forensic Genetics

Ludes¹,

Keyser²

2011

Forensic Medicine - From Old Problems to New Challenges

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“…The Royal Canadian Mounted Police (RCMP) National Forensic Laboratory Services implemented automated DNA extraction with DNA IQ™ paramagnetic beads (Promega Corporation) for biological samples in 2004-2005 [1][2][3]. This resulted in an enhanced throughput and capacity to produce high quality STR profiles.…”

Section: Introductionmentioning

confidence: 99%

“…Lysates were transferred robotically (with 700 µL of Promega Lysis Buffer; Promega Corporation, Madison, WI) with a TE-Shake Unit, a TE-VacS Unit and Teflon-coated stainless steel tips; TECAN US, Research Triangle Park, NC)[1][2][3]. The DNA was eluted from the beads at 65°C in a final volume of 52 or 60 μL of low Tris-EDTA (10 mM Tris pH 8.0, 0.1 mM EDTA) and stored at 4°C.For Experiments #3, 6, 9 and 10, DNA extraction was performed using a modified protocol based on the DNA IQ™ Casework Pro Kit and the Maxwell® 16 System (Promega Corporation).…”

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confidence: 99%