Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

Špoljarić, Branimira; Popović, Maja; Crnjac, Josip; Savatović, Zrinka Žderić; Ratko, Martina; Lozančić, Mateja; Jurak, Matea; Špoljarić, Igor; Špoljarić, Daniel; Mršić, Gordan

doi:10.1515/acve-2016-0016

Cited by 1 publication

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References 9 publications

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“…The eluted DNA is double stranded. Silica methods have been shown to produce a higher quality DNA with efficient removal of contaminants and inhibitors, while also working well on DNA recovery from degraded samples [131][132][133][134][135]. The samples irradiated at 9000 kGy gamma resulted in no STR/AMEL profiles for any sample and the quantification showed no DNA present.…”

Section: Nuclear Dna Analysismentioning

confidence: 99%

Potential effects of ionizing radiation on the evidentiary value of DNA, latent fingerprints, hair, and fibers: A comprehensive review and new results

Monson

Ali

Brandhagen

et al. 2018

Forensic Science International

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An extensive literature review and new post-irradiation experimental results are presented of genotyping blood stains and hair, and physical examinations of latent fingerprints, hairs, and fibers. Results indicate that successful development of nuclear short tandem repeat (STR) and mitochondrial DNA sequence profiles from human blood and hair evidence is possible-up to a point-following exposure to gamma, neutron, beta, and alpha radiation at several levels that would most likely be present at this type of crime scene (i.e., a "dirty bomb," etc.). Commencing at gamma radiation levels between 90 and 900kGy, DNA analysis using conventional DNA techniques was unsuccessful. In general, irradiation negatively affected the quality of latent fingerprints. All four radiation types degraded most fingerprint samples at all doses; nevertheless, many fingerprints remained of value for potential use in comparison. Although variable from one hair to another, microscopic changes observed for all types and levels of irradiation could potentially result in false exclusions. Negligible microscopic changes were observed in papers and fibers (used as substrates for fingerprints and bloodstains) up to 90kGy gamma, but fluorescence of fibers began to change above that dose. Paper and fibers, as well as plastic evidence enclosures, became extremely brittle leading to breakage after a gamma dose of 900kGy.

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