Validation of a Commercially Available Immunoassay for the Measurement of Bovine Cardiac Troponin I

Varga, Anita; Schober, Karsten E.; Walker, William L.; Lakritz, Jeffrey; Rings, D. Michael

doi:10.1111/j.1939-1676.2009.0256.x

Cited by 28 publications

(35 citation statements)

References 53 publications

(116 reference statements)

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“…cTnI is generally considered to be a stable molecule under various storage conditions. 4,17 The reason for the increase over time in [cTnI] i in the plasma with a low [cTnI] i stored at 20-248C in this study is unknown. The [cTnI] i in the three aliquots stored at each temperature was only measured once each day.…”

Section: Discussionmentioning

confidence: 88%

“…This is consistent with results in other species, including other ruminants. 3,11,17 In one study, [cTnI] measured by the ADVIA Centaur Tn-I Ultra was 0.03 ng/ml in each of 30 healthy dairy cows; [cTnI] was 0.01 ng/ml in 23 of the cows. 17 This is unsurprising, given that other markers for loss of cellular integrity (e.g., alanine aminotransferase) are present only in low to undetectable quantities in the blood of healthy animals.…”

Section: Discussionmentioning

confidence: 99%

“…16 Antibodies to human cTnI have been developed and are used in proprietary enzyme-linked immunosorbent assays (ELISAs) for measuring [cTnI] in blood. 10,17 One such assay system, the VetScan i-STAT 1 Handheld Analyzer and cTnI cartridges (i-STAT cTnI assay) (Abbott Point of Care Inc., Princeton, New Jersey 08540, USA) was used by Boesch et al to demonstrate that free-ranging white-tailed deer (Odocoileus virginianus) chemically immobilized with intramuscular butorphanol-medetomidine after physical restraint in Clover traps, had significantly greater [cTnI] than those that were ground-darted with similar doses of the same drugs. 5 The authors suggested that oxygen demand on cardiac muscle might have been increased by sympathetic discharge and hyperthermia as the deer struggled in the trap.…”

Section: Introductionmentioning

confidence: 98%

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LABORATORY VALIDATION OF A POINT-OF-CARE CARDIAC TROPONIN I ASSAY FOR USE IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS)

Boesch¹,

Gleed²,

Erb³

et al. 2015

Journal of Zoo and Wildlife Medicine

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The VetScan® i-STAT® 1 Handheld Analyzer and cardiac troponin I (cTnI) cartridges (i-STAT cTnI assay) measured greater median cTnI concentration [cTnI] in free-ranging white-tailed deer (Odocoileus virginianus) hand-injected with anesthetic drugs after physical restraint in Clover traps than in those ground-darted with the same drugs. This suggested that Clover trapping induces myocardial damage, bringing the use of this capture method under scrutiny. The purpose of this study was to confirm the validity of the i-STAT cTnI assay in deer before recommending changes in capture methods. Median [cTnI] measured by the i-STAT cTnI assay ([cTnI]i) in heparinized whole blood collected from 52 healthy, reproductively mature, female deer physically restrained in a chute was 0.01 ng/ml (10-90% percentiles: 0.00-0.03 ng/ml; minimum, maximum: 0.00, 0.07 ng/ml); [cTnI]i was 0.00 ng/ml in 42% of the deer. There was no association between [cTnI]i and either clotting or hemolytic index. [cTnI]i was 0.00 ng/ml when deer skeletal muscle homogenate was added to deer blood with [cTnI]i of 0.00 ng/ml, confirming the i-STAT cTnI assay does not detect skeletal muscle troponins. When deer cardiac muscle homogenate was serially diluted with 1) deer blood, 2) deer plasma, and 3) cow blood, [cTnI]i was directly proportional (Y intercept=-0.09, 0.7, and -0.08 ng/ml, respectively; r2≥0.97) to the fraction of homogenate in each sample. Deer cardiac muscle homogenate was diluted with deer blood to produce three samples with low, intermediate, and high [cTnI]i; serial measurements (n=10) performed on each sample yielded coefficients of variation (CVs) of 8, 20, and 11%, respectively. Corresponding CVs when plasma was used as diluent were 13, 9, and 7%, respectively. [cTnI]i increased when plasma with a low [cTnI]i was stored at 20-24°C for 9 days. Three freeze-thaw cycles caused no systematic change in plasma [cTnI]i.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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LABORATORY VALIDATION OF A POINT-OF-CARE CARDIAC TROPONIN I ASSAY FOR USE IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS)

Boesch¹,

Gleed²,

Erb³

et al. 2015

Journal of Zoo and Wildlife Medicine

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“…Cardiac troponin I (cTnI) is a more sensitive and persistent indicator of cardiac injury, with high tissue specificity, than other markers in the presence of marked skeletal muscle injury, liver disease, and chronic renal failure (O'Brien et al, 1997;Adin et al, 2005). The molecular structure of troponin proteins is highly conserved across species, and some current assays developed for their detection in humans have been used and validated in several other species (Oyama and Solter, 2004;Varga et al, 2009). The central hypothesis of this study is that acid-base and electrolyte disturbances associated with ARLA and secondary complications, lead to some degree of cardiovascular injury.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

Fartashvand

Hajisadeghi

2018

J Hellenic Vet Med Soc

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In this study we investigated the changes of cardiac injury biomarkers in serum samples from 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy controls belonging to the Ghezel breed. After clinical examination and recording of vital signs (heart rate, respiratory rate, rectal temperature and hydration status), rumen fluid and venous blood samples were collected. The pH of rumen fluid was determined using a paper tape pH-meter and lactic acid concentration of serum was measured using a commercially available method. Similarly, activities of AST, LDH, CK-MB and serum concentration of cTnI were measured using special commercial kits. According to the findings serum activities of AST (p = 0.007) and CK-MB (p = 0.002) in sheep with ARLA were significantly higher than in healthy animals. Serum LDH activity in the disease group was higher than in the control group, however this difference was statistically non-significant. cTnI concentration were 0.684 ± 0.03 ng/ml in sheep with ARLA, which was significantly higher than in healthy sheep (p = 0.000). There were significant negative correlations between ruminal pH and serum lactate levels, heart rate, respiratory rate and dehydration degree. The sheep with the lowest ruminal pH (3 cases with < 4.5) had the highest levels of cTnI (2.28 to 3.06 ng/mL), and all died. It can be concluded that lactic acidosis may cause some degree of heart damage, although further studies are needed to support this speculation.

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“…This presents an opportunity for field testing of large groups of cattle with rapid results that can aid in disease classification and outcome discrimination. Recently, serum cTn I has been reported for cattle having a variety of cardiogenic and non-cardiogenic disease [21][22][23][24][25][26][27].…”

mentioning

confidence: 99%

Assessment of Blood Concentration of Cardiac Troponin I (cTnI) in Healthy Calves and Stocker Calves Effected with Bovine Respiratory Disease Complex

Anderson¹,

White²,

Nickell³

et al. 2017

Journal of Veterinary Science and Animal Husbandry

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Validation of a Commercially Available Immunoassay for the Measurement of Bovine Cardiac Troponin I

Cited by 28 publications

References 53 publications

LABORATORY VALIDATION OF A POINT-OF-CARE CARDIAC TROPONIN I ASSAY FOR USE IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS)

LABORATORY VALIDATION OF A POINT-OF-CARE CARDIAC TROPONIN I ASSAY FOR USE IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS)

Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

Assessment of Blood Concentration of Cardiac Troponin I (cTnI) in Healthy Calves and Stocker Calves Effected with Bovine Respiratory Disease Complex

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