Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts

Development and validation of an HPLC-DAD method for quantification of bornesitol in extracts from Hancornia speciosa leaves after derivatization with p-toluenesulfonyl chloride

“…We have previously demonstrated the potential antihypertensive activity of an ethanol extract from H. speciosa leaves by in vitro inhibition of angiotensin I-converting enzyme [5] and vasodilator effects on rat aortic and mesenteric preparations [6,7]. Rutin and the cyclitol l-(+)-bornesitol ( Fig.…”

Characterization, antioxidant potential and cytotoxic study of mangaba fruits

“…Hancornia speciosa is traditionally used in the treatment of several diseases, including hypertension and infl ammatory diseases, mainly dermatites, rheumatism and hepatitis (SERRA et al, 2005;AGRA et al, 2007;BRANDÃO et al, 2011). The result of the phytochemical screening (Table 3) revealed that phenols, fl avonoids and tannins were present in the ethanolic extract of H. speciosa.…”

“…Among these fruits, the mangaba (Hancornia speciosa), a tropical fruit belonging to the Apocinaceas family, stands out and has a great potential for economic exploitation, as it is still consumed fresh and there are few manufactured products developed based on this fruit. SERRA et al (2005) showed that the ethanol extract of mangaba leaves exerts a hypotensive effect by inhibiting the angiotensinconverting enzyme (ACE), and FERREIRA et al (2007 a, b) demonstrated that this extract induces vasodilation through the production of nitric oxide, which is involved in the modulation of gastric mucosal integrity by adjusting the alkaline and acid secretions and the blood fl ow in the gastric mucosa. MORAES et al (2008) suggested in their studies that the effectiveness of mangaba in fi ghting and healing gastric ulcers is based on its ability to stimulate the synthesis of mucus, causing an anti-secretory effect and exerting an anti-Helicobacter pylori action without toxic effects.…”

Assay of Angiotensin I-converting Enzyme-inhibiting Activity Based on the Detection of 3-Hydroxybutyrate with Water-soluble Tetrazolium Salt

“…[10][11][12][13][14][15][16][17][18] The published data were over a wide range because the IC50 values were influenced by various factors, including the method conditions, the source of the enzyme, the calculation method, and the relationship between the inhibitor and the enzyme source. 1,11 Thus, it was difficult to compare the data directly, but the IC50 values recognized in this study did not differ vastly from the published data. Besides, there were some differences in the values of Tokuho A and C between the two methods.…”