Validation of a chromatographic method to routine analysis of trans-resveratrol and quercetin in red wines

Abstract

The objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit: specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines.

A SPE Method with Two MIPs in Two Steps for Improving the Selectivity of MIPs

“…In the static adsorption experiment, 10 mg of MIPs or NIPs was added into 10 mL tubes, respectively, and mixed with 3 mL of methanol solution containing known concentrations of quercitin. Then the mixtures were shaken at 90 rpm for 6 h. The mixtures were centrifuged at 8000 rpm for 15 min, and the supernatant solutions of quercetin were determined by using absorbance of 371 nm . The equilibrium adsorption capacity ( Q , μg/g) was calculated as follows: where C 0 (μg/mL) and C e (μg/mL) are the initial and equilibrium concentration of quercetin, respectively, V (mL) is the volume of quercetin solution, and m (g) is the mass of the adsorbent used (MIPs materials).…”

“…Then the mixtures were shaken at 90 rpm for 6 h. The mixtures were centrifuged at 8000 rpm for 15 min, and the supernatant solutions of quercetin were determined by using absorbance of 371 nm. 26 The equilibrium adsorption capacity (Q, μg/g) was calculated as follows:…”

Rapid, Highly-Sensitive and Ecologically Greener Reversed-Phase/Normal-Phase HPTLC Technique with Univariate Calibration for the Determination of Trans-Resveratrol

“…Some highperformance liquid chromatography (HPLC) assays are available for the quantification of TRV in polymeric nanoparticles, lipidic nanoemulsions and plant extracts [11][12][13][14]. Various HPLC assays are also available for the detection of TRV in different varieties of wine samples [15][16][17][18][19]. Various HPLC assays are also utilized for TRV analysis in the plasma samples of rats and humans [12,[20][21][22][23].…”

Voltammetric Determination of Total Phenolic Compounds in Cotton Leaves of Gossypium barbadense and Gossypium hirsutum Challenged with Spodoptera cosmioides (Walker)

“…13 Spectrophotometric and chromatographic methods coupled or not to mass spectrometers are the methods often used for the determination of the phenolic compounds in plant samples. [15][16][17][18][19] Voltammetric methods appear as a good alternative to phenolic compounds detection and quantification in a wide range of samples, including plants, showing advantages, such as high sensitivity; portability; low cost and present easy operation; they generate small amounts of chemical waste; the samples can be colored and they can be used for the antioxidant capacity evaluation in vitro. 17,[20][21][22][23][24][25][26][27] Voltammetry comprises a group of electroanalytical techniques, in which the information about the chemical species of interest is obtained through oxidation-reduction current as a function of the applied potential in an electrochemical cell.…”