“…2,3 For example, microfluidic large scale integration (LSI) uses microchannel networks with integrated pressure-actuated elastomeric pumps and valves to control, route, and mix fluid in channels for on-chip genetic analysis of single cells. 4 Others have used centrifugal forces, 5 surface acoustic waves (SAW), 6 and electrical forces to perform fluidic metering mixing and aliquoting for applications including DNA extraction, plasma separation, 7 cell lysis, 8 protein crystallization, 9 immunoassays, 10 and single cell analysis. 11 The majority of these microfluidic platforms, however, require large and often expensive external actuation equipment for operation, which can confine their usefulness to the laboratory a) Author to whom correspondence should be addressed.…”