Validation of a blood plasma separation system by biomarker detection

Kersaudy-Kerhoas, Maïwenn; Kavanagh, Deirdre M.; Dhariwal, R. S.; Campbell, Colin J.; Desmulliez, Marc Phillipe Yves

doi:10.1039/b926834k

Cited by 66 publications

(86 citation statements)

References 43 publications

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“…24 The chip comprises several constrictions enhancing the lateral drift force pushing blood cells (2-30 lm) to the channel centre while plasma is drawn off from cell-free zones through narrower side channels. As this lateral force is smaller for bacterial cells ($0.25-1 lm), these are relatively marginated, similar to the strategy adopted in Ref.…”

Section: Methodsmentioning

confidence: 99%

Impact of microfluidic processing on bacterial ribonucleic acid expression

et al. 2015

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Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.

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Section: Methodsmentioning

confidence: 99%

Impact of microfluidic processing on bacterial ribonucleic acid expression

et al. 2015

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“…A variety of microfluidic devices with the aim of integrating blood plasma extraction have been investigated and reviewed [41,43,44], thereby harnessing the advantages of miniaturized systems. Although, in comparison the yield of the presented system is lower, there are several possibilities to improve the performance, e.g., with a symmetric geometry [45], parallelization [46] or installing units in series [23,47].…”

Section: Human Blood Plasma Separationmentioning

confidence: 99%

Microfluidic Vortex Enhancement for on-Chip Sample Preparation

et al. 2015

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In the past decade a large amount of analysis techniques have been scaled down to the microfluidic level. However, in many cases the necessary sample preparation, such as separation, mixing and concentration, remains to be performed off-chip. This represents a major hurdle for the introduction of miniaturized sample-in/answer-out systems, preventing the exploitation of microfluidic's potential for small, rapid and accurate diagnostic products. New flow engineering methods are required to address this hitherto insufficiently studied aspect. One microfluidic tool that can be used to miniaturize and integrate sample preparation procedures are microvortices. They have been successfully applied as microcentrifuges, mixers, particle separators, to name but a few. In this work, we utilize a novel corner structure at a sudden channel expansion of a microfluidic chip to enhance the formation of a microvortex. For a maximum area of the microvortex, both chip geometry and corner structure were optimized with a computational fluid dynamic (CFD) model. Fluorescent particle trace measurements with the optimized design prove Micromachines 2015, 6 240 that the corner structure increases the size of the vortex. Furthermore, vortices are induced by the corner structure at low flow rates while no recirculation is observed without a corner structure. Finally, successful separation of plasma from human blood was accomplished, demonstrating a potential application for clinical sample preparation. The extracted plasma was characterized by a flow cytometer and compared to plasma obtained from a standard benchtop centrifuge and from chips without a corner structure.

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“…However, by traditional methods, not only does it require a large volume of the blood sample, but it also takes a long time and the separation process may result in the breaking of the blood cells and in return introducing unnecessary noise in the system. [1][2][3][4] Meanwhile, the advent of microfluidic devices provides a promising way to overcome these limitations. 5 One approach is to employ active separation forces such as centrifugation, [6][7][8] magnetophoresis, 9 dielectrophoresis (DEP), 10 and acoustic standing waves 11 to selectively separate a) Email: kwangoh@buffalo.edu 1932-1058/2015/9(1)/014106/12/$30.00 V C 2015 AIP Publishing LLC 9, 014106-1 blood cells from plasma.…”

Section: Introductionmentioning

confidence: 99%

Phaseguide-assisted blood separation microfluidic device for point-of-care applications

Lee

Pinheiro

et al. 2015

Biomicrofluidics

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We propose a blood separation microfluidic device suitable for point-of-care (POC) applications. By utilizing the high gas permeability of polydimethylsiloxane (PDMS) and phaseguide structures, a simple blood separation device is presented. The device consists of two main parts. A separation chamber with the phaseguide structures, where a sample inlet, a tape-sealed outlet, and a dead-end ring channel are connected, and pneumatic chambers, in which manually operating syringes are plugged. The separation chamber and pneumatic chambers are isolated by a thin PDMS wall. By manually pulling out the plunger of the syringe, a negative pressure is instantaneously generated inside the pneumatic chamber. Due to the gas diffusion from the separation chamber to the neighboring pneumatic chamber through the thin permeable PDMS wall, low pressure can be generated, and then the whole blood at the sample inlets starts to be drawn into the separation chamber and separated through the phaseguide structures. Reversely, after removing the tape at the outlet and manually pushing in the plunger of the syringe, a positive pressure will be created which will cause the air to diffuse back into the ring channel, and therefore allow the separated plasma to be recovered at the outlet on demand. In this paper, we focused on the study of the plasma separation and associated design parameters, such as the PDMS wall thickness, the air permeable overlap area between the separation and pneumatic chambers, and the geometry of the phaseguides. The device required only 2 ll of whole blood but yielding approximately 0.38 ll of separated plasma within 12 min. Without any of the requirements of sophisticated equipment or dilution techniques, we can not only separate the plasma from the whole blood for on-chip analysis but also can push out only the separated plasma to the outlet for off-chip analysis. V C 2015 AIP Publishing LLC.

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Validation of a blood plasma separation system by biomarker detection

Cited by 66 publications

References 43 publications

Impact of microfluidic processing on bacterial ribonucleic acid expression

Impact of microfluidic processing on bacterial ribonucleic acid expression

Microfluidic Vortex Enhancement for on-Chip Sample Preparation

Phaseguide-assisted blood separation microfluidic device for point-of-care applications

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