Impact of microfluidic processing on bacterial ribonucleic acid expression

Abstract

Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.

Calcium transcriptionally regulates movement, recombination and other functions of Xylella fastidiosa under constant flow inside microfluidic chambers

“…These results suggest our MC system is suitable for whole transcriptome analysis of X. fastidiosa. Recently, microfluidic systems have been demonstrated to be suitable diagnostic devices measuring RNA expression of bacteria (by microarray) in human blood (Gandi et al, 2015). Highthroughput microfluidics were used to assess the genome of a single bacterial cell (Pamp et al, 2012) and transcriptome of a single mammalian cell (Tang et al, 2009;Streets et al, 2014).…”