Validation and Optimization of Polyvinyl Alcohol-Functionalized Gold Nanoparticles for Producing Lactose-Free Dairy Products

Alshanberi, Asim Muhammed; Ansari, Shakeel Ahmed

doi:10.13005/ojc/370317

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Functionalized AuNPs can immobilize enzymes via covalent bonding, electrostatic interactions, or physical adsorption. One advantage of using AuNPs is the ease of detecting and quantifying immobilized enzymes using techniques such as UV-visible spectroscopy [86].…”

Section: Gold Nanoparticles Modificationmentioning

confidence: 99%

Green Synthesis of Biocatalysts Based on Nanocarriers Promises an Effective Role in Pharmaceutical and Biomedical Fields

Khafaga,

Radwan,

Muteeb

et al. 2023

Catalysts

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Nanobiocatalysts (NBCs) are a promising new class of biocatalysts that combine the advantages of enzymes and nanomaterials. Enzymes are biological catalysts that are highly selective and efficient, but they can be unstable in harsh environments. Nanomaterials, on the other hand, are small particles with unique properties that can improve the stability, activity, and selectivity of enzymes. The development of NBCs has been driven by the need for more sustainable and environmentally friendly bioprocessing methods. Enzymes are inherently green catalysts, but they can be expensive and difficult to recover and reuse. NBCs can address these challenges by providing a stable and reusable platform for enzymes. One of the key challenges in the development of NBCs is the immobilization of enzymes on nanomaterials. Enzyme immobilization is a process that attaches enzymes to a solid support, which can protect the enzymes from harsh environments and make them easier to recover and reuse. There are many different methods for immobilizing enzymes, and the choice of method depends on the specific enzyme and nanomaterial being used. This review explores the effective role of NBCs in pharmaceutical and biomedical fields.

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Section: Gold Nanoparticles Modificationmentioning

confidence: 99%

Green Synthesis of Biocatalysts Based on Nanocarriers Promises an Effective Role in Pharmaceutical and Biomedical Fields

Khafaga,

Radwan,

Muteeb

et al. 2023

Catalysts

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Recent Advances and Perspectives on Food-Grade Immobilisation Systems for Enzymes

Chalella Mazzocato,

Jacquier

2024

Foods

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The use of enzyme immobilisation is becoming increasingly popular in beverage processing, as this method offers significant advantages, such as enhanced enzyme performance and expanded applications, while allowing for easy process termination via simple filtration. This literature review analysed approximately 120 articles, published on the Web of Science between 2000 and 2023, focused on enzyme immobilisation systems for beverage processing applications. The impact of immobilisation on enzymatic activity, including the effects on the chemical and kinetic properties, recyclability, and feasibility in continuous processes, was evaluated. Applications of these systems to beverage production, such as wine, beer, fruit juices, milk, and plant-based beverages, were examined. The immobilisation process effectively enhanced the pH and thermal stability but caused negative impacts on the kinetic properties by reducing the maximum velocity and Michaelis–Menten constant. However, it allowed for multiple reuses and facilitated continuous flow processes. The encapsulation also allowed for easy process control by simplifying the removal of the enzymes from the beverages via simple filtration, negating the need for expensive heat treatments, which could result in product quality losses.

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Validation and Optimization of Polyvinyl Alcohol-Functionalized Gold Nanoparticles for Producing Lactose-Free Dairy Products

Cited by 2 publications

References 28 publications

Green Synthesis of Biocatalysts Based on Nanocarriers Promises an Effective Role in Pharmaceutical and Biomedical Fields

Green Synthesis of Biocatalysts Based on Nanocarriers Promises an Effective Role in Pharmaceutical and Biomedical Fields

Recent Advances and Perspectives on Food-Grade Immobilisation Systems for Enzymes

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